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CRISPR/Cas9 systems have off-target activity with insertions or deletions between target DNA and guide RNA sequences
CRISPR/Cas9 systems are a versatile tool for genome editing due to the highly efficient targeting of DNA sequences complementary to their RNA guide strands. However, it has been shown that RNA-guided Cas9 nuclease cleaves genomic DNA sequences containing mismatches to the guide strand. A better unde...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4066799/ https://www.ncbi.nlm.nih.gov/pubmed/24838573 http://dx.doi.org/10.1093/nar/gku402 |