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Genomic Analysis of the Appearance of Ovarian Mast Cells in Neonatal MRL/MpJ Mice

In MRL/MpJ mice, ovarian mast cells (OMCs) are more abundant than in other mouse strains, and tend to distribute beneath the ovarian surface epithelium at birth. This study investigated the factors regulating the appearance of neonatal OMCs in progeny of the cross between MRL/MpJ and C57BL/6N strain...

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Autores principales: Nakamura, Teppei, Sakata, Yuko, Otsuka-Kanazawa, Saori, Ichii, Osamu, Chihara, Masataka, Nagasaki, Ken-ichi, Namiki, Yuka, Kon, Yasuhiro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4067331/
https://www.ncbi.nlm.nih.gov/pubmed/24956472
http://dx.doi.org/10.1371/journal.pone.0100617
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author Nakamura, Teppei
Sakata, Yuko
Otsuka-Kanazawa, Saori
Ichii, Osamu
Chihara, Masataka
Nagasaki, Ken-ichi
Namiki, Yuka
Kon, Yasuhiro
author_facet Nakamura, Teppei
Sakata, Yuko
Otsuka-Kanazawa, Saori
Ichii, Osamu
Chihara, Masataka
Nagasaki, Ken-ichi
Namiki, Yuka
Kon, Yasuhiro
author_sort Nakamura, Teppei
collection PubMed
description In MRL/MpJ mice, ovarian mast cells (OMCs) are more abundant than in other mouse strains, and tend to distribute beneath the ovarian surface epithelium at birth. This study investigated the factors regulating the appearance of neonatal OMCs in progeny of the cross between MRL/MpJ and C57BL/6N strains. F1 neonates had less than half the number of OMCs than MRL/MpJ. Interestingly, MRLB6F1 had more neonatal OMCs than B6MRLF1, although they were distributed over comparable areas. Furthermore, in MRL/MpJ fetuses for which parturition was delayed until embryonic day 21.5, the number of OMCs was significantly higher than in age-matched controls at postnatal day 2. These results suggest that the number of OMCs was influenced by the environmental factors during pregnancy. Quantitative trait locus analysis using N2 backcross progeny revealed two significant loci on chromosome 8: D8Mit343–D8Mit312 for the number of OMCs and D8Mit86–D8Mit89 for their distribution, designated as mast cell in the ovary of MRL/MpJ 1 (mcom1) and mcom2, respectively. Among MC migration-associated genes, ovarian expression of chemokine (C-C motif) ligand 17 at mcom1 locus was significantly higher in MRL/MpJ than in C57BL/6N, and positively correlated with the expression of OMC marker genes. These results indicate that the appearance of neonatal OMCs in MRL/MpJ is controlled by environmental factors and filial genetic factors, and that the abundance and distribution of OMCs are regulated by independent filial genetic elements.
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spelling pubmed-40673312014-06-25 Genomic Analysis of the Appearance of Ovarian Mast Cells in Neonatal MRL/MpJ Mice Nakamura, Teppei Sakata, Yuko Otsuka-Kanazawa, Saori Ichii, Osamu Chihara, Masataka Nagasaki, Ken-ichi Namiki, Yuka Kon, Yasuhiro PLoS One Research Article In MRL/MpJ mice, ovarian mast cells (OMCs) are more abundant than in other mouse strains, and tend to distribute beneath the ovarian surface epithelium at birth. This study investigated the factors regulating the appearance of neonatal OMCs in progeny of the cross between MRL/MpJ and C57BL/6N strains. F1 neonates had less than half the number of OMCs than MRL/MpJ. Interestingly, MRLB6F1 had more neonatal OMCs than B6MRLF1, although they were distributed over comparable areas. Furthermore, in MRL/MpJ fetuses for which parturition was delayed until embryonic day 21.5, the number of OMCs was significantly higher than in age-matched controls at postnatal day 2. These results suggest that the number of OMCs was influenced by the environmental factors during pregnancy. Quantitative trait locus analysis using N2 backcross progeny revealed two significant loci on chromosome 8: D8Mit343–D8Mit312 for the number of OMCs and D8Mit86–D8Mit89 for their distribution, designated as mast cell in the ovary of MRL/MpJ 1 (mcom1) and mcom2, respectively. Among MC migration-associated genes, ovarian expression of chemokine (C-C motif) ligand 17 at mcom1 locus was significantly higher in MRL/MpJ than in C57BL/6N, and positively correlated with the expression of OMC marker genes. These results indicate that the appearance of neonatal OMCs in MRL/MpJ is controlled by environmental factors and filial genetic factors, and that the abundance and distribution of OMCs are regulated by independent filial genetic elements. Public Library of Science 2014-06-23 /pmc/articles/PMC4067331/ /pubmed/24956472 http://dx.doi.org/10.1371/journal.pone.0100617 Text en © 2014 Nakamura et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Nakamura, Teppei
Sakata, Yuko
Otsuka-Kanazawa, Saori
Ichii, Osamu
Chihara, Masataka
Nagasaki, Ken-ichi
Namiki, Yuka
Kon, Yasuhiro
Genomic Analysis of the Appearance of Ovarian Mast Cells in Neonatal MRL/MpJ Mice
title Genomic Analysis of the Appearance of Ovarian Mast Cells in Neonatal MRL/MpJ Mice
title_full Genomic Analysis of the Appearance of Ovarian Mast Cells in Neonatal MRL/MpJ Mice
title_fullStr Genomic Analysis of the Appearance of Ovarian Mast Cells in Neonatal MRL/MpJ Mice
title_full_unstemmed Genomic Analysis of the Appearance of Ovarian Mast Cells in Neonatal MRL/MpJ Mice
title_short Genomic Analysis of the Appearance of Ovarian Mast Cells in Neonatal MRL/MpJ Mice
title_sort genomic analysis of the appearance of ovarian mast cells in neonatal mrl/mpj mice
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4067331/
https://www.ncbi.nlm.nih.gov/pubmed/24956472
http://dx.doi.org/10.1371/journal.pone.0100617
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