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DNA content of a functioning chicken kinetochore
In order to understand the three-dimensional structure of the functional kinetochore in vertebrates, we require a complete list and stoichiometry for the protein components of the kinetochore, which can be provided by genetic and proteomic experiments. We also need to know how the chromatin-containi...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Netherlands
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4067537/ https://www.ncbi.nlm.nih.gov/pubmed/24633498 http://dx.doi.org/10.1007/s10577-014-9410-3 |
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author | Ribeiro, Susana Abreu Vagnarelli, Paola Earnshaw, William C. |
author_facet | Ribeiro, Susana Abreu Vagnarelli, Paola Earnshaw, William C. |
author_sort | Ribeiro, Susana Abreu |
collection | PubMed |
description | In order to understand the three-dimensional structure of the functional kinetochore in vertebrates, we require a complete list and stoichiometry for the protein components of the kinetochore, which can be provided by genetic and proteomic experiments. We also need to know how the chromatin-containing CENP-A, which makes up the structural foundation for the kinetochore, is folded, and how much of that DNA is involved in assembling the kinetochore. In this MS, we demonstrate that functioning metaphase kinetochores in chicken DT40 cells contain roughly 50 kb of DNA, an amount that corresponds extremely closely to the length of chromosomal DNA associated with CENP-A in ChIP-seq experiments. Thus, during kinetochore assembly, CENP-A chromatin is compacted into the inner kinetochore plate without including significant amounts of flanking pericentromeric heterochromatin. |
format | Online Article Text |
id | pubmed-4067537 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Springer Netherlands |
record_format | MEDLINE/PubMed |
spelling | pubmed-40675372014-07-02 DNA content of a functioning chicken kinetochore Ribeiro, Susana Abreu Vagnarelli, Paola Earnshaw, William C. Chromosome Res Original Research In order to understand the three-dimensional structure of the functional kinetochore in vertebrates, we require a complete list and stoichiometry for the protein components of the kinetochore, which can be provided by genetic and proteomic experiments. We also need to know how the chromatin-containing CENP-A, which makes up the structural foundation for the kinetochore, is folded, and how much of that DNA is involved in assembling the kinetochore. In this MS, we demonstrate that functioning metaphase kinetochores in chicken DT40 cells contain roughly 50 kb of DNA, an amount that corresponds extremely closely to the length of chromosomal DNA associated with CENP-A in ChIP-seq experiments. Thus, during kinetochore assembly, CENP-A chromatin is compacted into the inner kinetochore plate without including significant amounts of flanking pericentromeric heterochromatin. Springer Netherlands 2014-03-15 2014 /pmc/articles/PMC4067537/ /pubmed/24633498 http://dx.doi.org/10.1007/s10577-014-9410-3 Text en © The Author(s) 2014 https://creativecommons.org/licenses/by/4.0/ Open AccessThis article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited. |
spellingShingle | Original Research Ribeiro, Susana Abreu Vagnarelli, Paola Earnshaw, William C. DNA content of a functioning chicken kinetochore |
title | DNA content of a functioning chicken kinetochore |
title_full | DNA content of a functioning chicken kinetochore |
title_fullStr | DNA content of a functioning chicken kinetochore |
title_full_unstemmed | DNA content of a functioning chicken kinetochore |
title_short | DNA content of a functioning chicken kinetochore |
title_sort | dna content of a functioning chicken kinetochore |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4067537/ https://www.ncbi.nlm.nih.gov/pubmed/24633498 http://dx.doi.org/10.1007/s10577-014-9410-3 |
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