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Electroacupuncture Improves Trinitrobenzene Sulfonic Acid-Induced Colitis, Evaluated by Transcriptomic Study

Inflammatory bowel disease is a chronic colonic inflammation that displays symptoms like diarrhea and weight loss. Acupuncture has been widely accepted by Western countries for the treatment of pain. Here, we analyzed efficacy and mechanism of electroacupuncture (EA) on trinitrobenzene sulfonic acid...

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Autores principales: Ho, Tin-Yun, Lo, Hsin-Yi, Chao, De-Cheng, Li, Chia-Cheng, Liu, Jau-Jin, Lin, Chingju, Hsiang, Chien-Yun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4068094/
https://www.ncbi.nlm.nih.gov/pubmed/24995035
http://dx.doi.org/10.1155/2014/942196
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author Ho, Tin-Yun
Lo, Hsin-Yi
Chao, De-Cheng
Li, Chia-Cheng
Liu, Jau-Jin
Lin, Chingju
Hsiang, Chien-Yun
author_facet Ho, Tin-Yun
Lo, Hsin-Yi
Chao, De-Cheng
Li, Chia-Cheng
Liu, Jau-Jin
Lin, Chingju
Hsiang, Chien-Yun
author_sort Ho, Tin-Yun
collection PubMed
description Inflammatory bowel disease is a chronic colonic inflammation that displays symptoms like diarrhea and weight loss. Acupuncture has been widely accepted by Western countries for the treatment of pain. Here, we analyzed efficacy and mechanism of electroacupuncture (EA) on trinitrobenzene sulfonic acid- (TNBS-) induced colitis in mice. Mice were intrarectally administered with 250 mg/kg TNBS and electroacupunctured at Quze (PC3) and Neiguan (PC6) acupoints, which have been applied for gastrointestinal disorders. Gene expression profiles in colons and spleens were analyzed by microarray for the elucidation of mechanism of EA. Our data showed that EA at PC3 and PC6 improved macroscopic and microscopic features of colitis and the improvement displayed a frequency-dependent manner. Administration of TNBS upregulated the expression of most cytokine genes in colons, while EA downregulated the expression of TNBS-induced cytokine genes. Pathway analysis showed that EA significantly affected inflammatory pathways in colons and immunity-associated pathway in spleens. Immunohistochemical staining further showed that EA decreased the expression of interleukin-1β and nuclear factor-κB. In conclusion, this is the first study reporting the global gene expression profiles of EA on TNBS-induced colitis. Our findings suggested that inflammatory and immunity pathways were involved in the anti-inflammatory mechanism of EA on colitis induced by TNBS.
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spelling pubmed-40680942014-07-03 Electroacupuncture Improves Trinitrobenzene Sulfonic Acid-Induced Colitis, Evaluated by Transcriptomic Study Ho, Tin-Yun Lo, Hsin-Yi Chao, De-Cheng Li, Chia-Cheng Liu, Jau-Jin Lin, Chingju Hsiang, Chien-Yun Evid Based Complement Alternat Med Research Article Inflammatory bowel disease is a chronic colonic inflammation that displays symptoms like diarrhea and weight loss. Acupuncture has been widely accepted by Western countries for the treatment of pain. Here, we analyzed efficacy and mechanism of electroacupuncture (EA) on trinitrobenzene sulfonic acid- (TNBS-) induced colitis in mice. Mice were intrarectally administered with 250 mg/kg TNBS and electroacupunctured at Quze (PC3) and Neiguan (PC6) acupoints, which have been applied for gastrointestinal disorders. Gene expression profiles in colons and spleens were analyzed by microarray for the elucidation of mechanism of EA. Our data showed that EA at PC3 and PC6 improved macroscopic and microscopic features of colitis and the improvement displayed a frequency-dependent manner. Administration of TNBS upregulated the expression of most cytokine genes in colons, while EA downregulated the expression of TNBS-induced cytokine genes. Pathway analysis showed that EA significantly affected inflammatory pathways in colons and immunity-associated pathway in spleens. Immunohistochemical staining further showed that EA decreased the expression of interleukin-1β and nuclear factor-κB. In conclusion, this is the first study reporting the global gene expression profiles of EA on TNBS-induced colitis. Our findings suggested that inflammatory and immunity pathways were involved in the anti-inflammatory mechanism of EA on colitis induced by TNBS. Hindawi Publishing Corporation 2014 2014-06-09 /pmc/articles/PMC4068094/ /pubmed/24995035 http://dx.doi.org/10.1155/2014/942196 Text en Copyright © 2014 Tin-Yun Ho et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Ho, Tin-Yun
Lo, Hsin-Yi
Chao, De-Cheng
Li, Chia-Cheng
Liu, Jau-Jin
Lin, Chingju
Hsiang, Chien-Yun
Electroacupuncture Improves Trinitrobenzene Sulfonic Acid-Induced Colitis, Evaluated by Transcriptomic Study
title Electroacupuncture Improves Trinitrobenzene Sulfonic Acid-Induced Colitis, Evaluated by Transcriptomic Study
title_full Electroacupuncture Improves Trinitrobenzene Sulfonic Acid-Induced Colitis, Evaluated by Transcriptomic Study
title_fullStr Electroacupuncture Improves Trinitrobenzene Sulfonic Acid-Induced Colitis, Evaluated by Transcriptomic Study
title_full_unstemmed Electroacupuncture Improves Trinitrobenzene Sulfonic Acid-Induced Colitis, Evaluated by Transcriptomic Study
title_short Electroacupuncture Improves Trinitrobenzene Sulfonic Acid-Induced Colitis, Evaluated by Transcriptomic Study
title_sort electroacupuncture improves trinitrobenzene sulfonic acid-induced colitis, evaluated by transcriptomic study
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4068094/
https://www.ncbi.nlm.nih.gov/pubmed/24995035
http://dx.doi.org/10.1155/2014/942196
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