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One-step assembly and targeted integration of multigene constructs assisted by the I-SceI meganuclease in Saccharomyces cerevisiae

In vivo assembly of overlapping fragments by homologous recombination in Saccharomyces cerevisiae is a powerful method to engineer large DNA constructs. Whereas most in vivo assembly methods reported to date result in circular vectors, stable integrated constructs are often preferred for metabolic e...

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Detalles Bibliográficos
Autores principales:	Kuijpers, Niels GA, Chroumpi, Soultana, Vos, Tim, Solis-Escalante, Daniel, Bosman, Lizanne, Pronk, Jack T, Daran, Jean-Marc, Daran-Lapujade, Pascale
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BlackWell Publishing Ltd 2013
Materias:	Research Articles
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4068284/ https://www.ncbi.nlm.nih.gov/pubmed/24028550 http://dx.doi.org/10.1111/1567-1364.12087

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