A novel Streptomyces spp. integration vector derived from the S. venezuelae phage, SV1

BACKGROUND: Integrating vectors based on the int/attP loci of temperate phages are convenient and used widely, particularly for cloning genes in Streptomyces spp. RESULTS: We have constructed and tested a novel integrating vector based on g27, encoding integrase, and attP site from the phage, SV1. T...

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Detalles Bibliográficos
Autores principales: Fayed, Bahgat, Younger, Ellen, Taylor, Gabrielle, Smith, Margaret C M
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2014
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4068962/
https://www.ncbi.nlm.nih.gov/pubmed/24885867
http://dx.doi.org/10.1186/1472-6750-14-51
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author Fayed, Bahgat
Younger, Ellen
Taylor, Gabrielle
Smith, Margaret C M
author_facet Fayed, Bahgat
Younger, Ellen
Taylor, Gabrielle
Smith, Margaret C M
author_sort Fayed, Bahgat
collection PubMed
description BACKGROUND: Integrating vectors based on the int/attP loci of temperate phages are convenient and used widely, particularly for cloning genes in Streptomyces spp. RESULTS: We have constructed and tested a novel integrating vector based on g27, encoding integrase, and attP site from the phage, SV1. This plasmid, pBF3 integrates efficiently in S. coelicolor and S. lividans but surprisingly fails to generate stable integrants in S. venezuelae, the natural host for phage SV1. CONCLUSION: pBF3 promises to be a useful addition to the range of integrating vectors currently available for Streptomyces molecular genetics.
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spelling pubmed-40689622014-06-25 A novel Streptomyces spp. integration vector derived from the S. venezuelae phage, SV1 Fayed, Bahgat Younger, Ellen Taylor, Gabrielle Smith, Margaret C M BMC Biotechnol Research Article BACKGROUND: Integrating vectors based on the int/attP loci of temperate phages are convenient and used widely, particularly for cloning genes in Streptomyces spp. RESULTS: We have constructed and tested a novel integrating vector based on g27, encoding integrase, and attP site from the phage, SV1. This plasmid, pBF3 integrates efficiently in S. coelicolor and S. lividans but surprisingly fails to generate stable integrants in S. venezuelae, the natural host for phage SV1. CONCLUSION: pBF3 promises to be a useful addition to the range of integrating vectors currently available for Streptomyces molecular genetics. BioMed Central 2014-05-30 /pmc/articles/PMC4068962/ /pubmed/24885867 http://dx.doi.org/10.1186/1472-6750-14-51 Text en Copyright © 2014 Fayed et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Fayed, Bahgat
Younger, Ellen
Taylor, Gabrielle
Smith, Margaret C M
A novel Streptomyces spp. integration vector derived from the S. venezuelae phage, SV1
title A novel Streptomyces spp. integration vector derived from the S. venezuelae phage, SV1
title_full A novel Streptomyces spp. integration vector derived from the S. venezuelae phage, SV1
title_fullStr A novel Streptomyces spp. integration vector derived from the S. venezuelae phage, SV1
title_full_unstemmed A novel Streptomyces spp. integration vector derived from the S. venezuelae phage, SV1
title_short A novel Streptomyces spp. integration vector derived from the S. venezuelae phage, SV1
title_sort novel streptomyces spp. integration vector derived from the s. venezuelae phage, sv1
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4068962/
https://www.ncbi.nlm.nih.gov/pubmed/24885867
http://dx.doi.org/10.1186/1472-6750-14-51
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