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MiR-133b Regulates the Expression of the Actin Protein TAGLN2 during Oocyte Growth and Maturation: A Potential Target for Infertility Therapy

Infertility is an area of increasing in life science research. Although follicular maturation disorders and anovulation are the primary causations of infertility, its molecular mechanism is not well understood. Recent research has shown that microRNAs (miRNAs) might play an important role in the reg...

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Autores principales: Xiao, Guohong, Xia, Chenglai, Yang, Jie, Liu, Jianqiao, Du, Hongzi, Kang, Xiangjin, Lin, Yuyi, Guan, Ronghua, Yan, Pengke, Tang, Shengsong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4069098/
https://www.ncbi.nlm.nih.gov/pubmed/24959893
http://dx.doi.org/10.1371/journal.pone.0100751
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author Xiao, Guohong
Xia, Chenglai
Yang, Jie
Liu, Jianqiao
Du, Hongzi
Kang, Xiangjin
Lin, Yuyi
Guan, Ronghua
Yan, Pengke
Tang, Shengsong
author_facet Xiao, Guohong
Xia, Chenglai
Yang, Jie
Liu, Jianqiao
Du, Hongzi
Kang, Xiangjin
Lin, Yuyi
Guan, Ronghua
Yan, Pengke
Tang, Shengsong
author_sort Xiao, Guohong
collection PubMed
description Infertility is an area of increasing in life science research. Although follicular maturation disorders and anovulation are the primary causations of infertility, its molecular mechanism is not well understood. Recent research has shown that microRNAs (miRNAs) might play an important role in the regulation of ovarian follicle development and maturation. In this study, the expression of miRNAs in metaphase I (MI) oocytes treated with or without insulin-like growth factor 1 (IGF-1) was observed by microRNA microarray analysis. Results show that 145 miRNAs were up-regulated and 200 miRNAs were down-regulated in MI oocytes after IGF-1 treatment. MiR-133b, which was up-regulated more than 30-fold, was chosen for further research. As a potential target of miR133b, transgelin 2 (TAGLN2) gene was down-regulated, at both transcription and translation levels, in miR-133b- over-expressed 293T cells, but TAGLN2 was up-regulated when the expression of miR-133b was inhibited. Furthermore, the expression level of TAGLN2 in the ovaries of 8-week- old mice was higher than that observed in 4-week-old mice. Immunofluorescence experiments showed that TAGLN2 was located in the cytoplasm. In general, our results indicate that miR-133b may play important roles in the growth and maturation of oocytes by regulating its potential target, TAGLN2, at both transcription and translation levels. Therefore, our research provides a potential new target for infertility therapy.
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spelling pubmed-40690982014-06-27 MiR-133b Regulates the Expression of the Actin Protein TAGLN2 during Oocyte Growth and Maturation: A Potential Target for Infertility Therapy Xiao, Guohong Xia, Chenglai Yang, Jie Liu, Jianqiao Du, Hongzi Kang, Xiangjin Lin, Yuyi Guan, Ronghua Yan, Pengke Tang, Shengsong PLoS One Research Article Infertility is an area of increasing in life science research. Although follicular maturation disorders and anovulation are the primary causations of infertility, its molecular mechanism is not well understood. Recent research has shown that microRNAs (miRNAs) might play an important role in the regulation of ovarian follicle development and maturation. In this study, the expression of miRNAs in metaphase I (MI) oocytes treated with or without insulin-like growth factor 1 (IGF-1) was observed by microRNA microarray analysis. Results show that 145 miRNAs were up-regulated and 200 miRNAs were down-regulated in MI oocytes after IGF-1 treatment. MiR-133b, which was up-regulated more than 30-fold, was chosen for further research. As a potential target of miR133b, transgelin 2 (TAGLN2) gene was down-regulated, at both transcription and translation levels, in miR-133b- over-expressed 293T cells, but TAGLN2 was up-regulated when the expression of miR-133b was inhibited. Furthermore, the expression level of TAGLN2 in the ovaries of 8-week- old mice was higher than that observed in 4-week-old mice. Immunofluorescence experiments showed that TAGLN2 was located in the cytoplasm. In general, our results indicate that miR-133b may play important roles in the growth and maturation of oocytes by regulating its potential target, TAGLN2, at both transcription and translation levels. Therefore, our research provides a potential new target for infertility therapy. Public Library of Science 2014-06-24 /pmc/articles/PMC4069098/ /pubmed/24959893 http://dx.doi.org/10.1371/journal.pone.0100751 Text en © 2014 Xiao et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Xiao, Guohong
Xia, Chenglai
Yang, Jie
Liu, Jianqiao
Du, Hongzi
Kang, Xiangjin
Lin, Yuyi
Guan, Ronghua
Yan, Pengke
Tang, Shengsong
MiR-133b Regulates the Expression of the Actin Protein TAGLN2 during Oocyte Growth and Maturation: A Potential Target for Infertility Therapy
title MiR-133b Regulates the Expression of the Actin Protein TAGLN2 during Oocyte Growth and Maturation: A Potential Target for Infertility Therapy
title_full MiR-133b Regulates the Expression of the Actin Protein TAGLN2 during Oocyte Growth and Maturation: A Potential Target for Infertility Therapy
title_fullStr MiR-133b Regulates the Expression of the Actin Protein TAGLN2 during Oocyte Growth and Maturation: A Potential Target for Infertility Therapy
title_full_unstemmed MiR-133b Regulates the Expression of the Actin Protein TAGLN2 during Oocyte Growth and Maturation: A Potential Target for Infertility Therapy
title_short MiR-133b Regulates the Expression of the Actin Protein TAGLN2 during Oocyte Growth and Maturation: A Potential Target for Infertility Therapy
title_sort mir-133b regulates the expression of the actin protein tagln2 during oocyte growth and maturation: a potential target for infertility therapy
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4069098/
https://www.ncbi.nlm.nih.gov/pubmed/24959893
http://dx.doi.org/10.1371/journal.pone.0100751
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