Rapid Adaptive Optical Recovery of Optimal Resolution over LargeVolumes

Using a de-scanned, laser-induced guide star and direct wavefront sensing, we demonstrate adaptive correction of complex optical aberrations at high numerical aperture and a 14 ms update rate. This permits us to compensate for the rapid spatial variation in aberration often encountered in biological...

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Detalles Bibliográficos
Autores principales: Wang, Kai, Milkie, Dan, Saxena, Ankur, Engerer, Peter, Misgeld, Thomas, Bronner, Marianne E., Mumm, Jeff, Betzig, Eric
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2014
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4069208/
https://www.ncbi.nlm.nih.gov/pubmed/24727653
http://dx.doi.org/10.1038/nmeth.2925
Descripción
Sumario:Using a de-scanned, laser-induced guide star and direct wavefront sensing, we demonstrate adaptive correction of complex optical aberrations at high numerical aperture and a 14 ms update rate. This permits us to compensate for the rapid spatial variation in aberration often encountered in biological specimens, and recover diffraction-limited imaging over large (> 240 μm)(3) volumes. We applied this to image fine neuronal processes and subcellular dynamics within the zebrafish brain.

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