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Rapid Adaptive Optical Recovery of Optimal Resolution over LargeVolumes
Using a de-scanned, laser-induced guide star and direct wavefront sensing, we demonstrate adaptive correction of complex optical aberrations at high numerical aperture and a 14 ms update rate. This permits us to compensate for the rapid spatial variation in aberration often encountered in biological...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4069208/ https://www.ncbi.nlm.nih.gov/pubmed/24727653 http://dx.doi.org/10.1038/nmeth.2925 |
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author | Wang, Kai Milkie, Dan Saxena, Ankur Engerer, Peter Misgeld, Thomas Bronner, Marianne E. Mumm, Jeff Betzig, Eric |
author_facet | Wang, Kai Milkie, Dan Saxena, Ankur Engerer, Peter Misgeld, Thomas Bronner, Marianne E. Mumm, Jeff Betzig, Eric |
author_sort | Wang, Kai |
collection | PubMed |
description | Using a de-scanned, laser-induced guide star and direct wavefront sensing, we demonstrate adaptive correction of complex optical aberrations at high numerical aperture and a 14 ms update rate. This permits us to compensate for the rapid spatial variation in aberration often encountered in biological specimens, and recover diffraction-limited imaging over large (> 240 μm)(3) volumes. We applied this to image fine neuronal processes and subcellular dynamics within the zebrafish brain. |
format | Online Article Text |
id | pubmed-4069208 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
record_format | MEDLINE/PubMed |
spelling | pubmed-40692082014-12-01 Rapid Adaptive Optical Recovery of Optimal Resolution over LargeVolumes Wang, Kai Milkie, Dan Saxena, Ankur Engerer, Peter Misgeld, Thomas Bronner, Marianne E. Mumm, Jeff Betzig, Eric Nat Methods Article Using a de-scanned, laser-induced guide star and direct wavefront sensing, we demonstrate adaptive correction of complex optical aberrations at high numerical aperture and a 14 ms update rate. This permits us to compensate for the rapid spatial variation in aberration often encountered in biological specimens, and recover diffraction-limited imaging over large (> 240 μm)(3) volumes. We applied this to image fine neuronal processes and subcellular dynamics within the zebrafish brain. 2014-04-13 2014-06 /pmc/articles/PMC4069208/ /pubmed/24727653 http://dx.doi.org/10.1038/nmeth.2925 Text en http://www.nature.com/authors/editorial_policies/license.html#terms Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms |
spellingShingle | Article Wang, Kai Milkie, Dan Saxena, Ankur Engerer, Peter Misgeld, Thomas Bronner, Marianne E. Mumm, Jeff Betzig, Eric Rapid Adaptive Optical Recovery of Optimal Resolution over LargeVolumes |
title | Rapid Adaptive Optical Recovery of Optimal Resolution over LargeVolumes |
title_full | Rapid Adaptive Optical Recovery of Optimal Resolution over LargeVolumes |
title_fullStr | Rapid Adaptive Optical Recovery of Optimal Resolution over LargeVolumes |
title_full_unstemmed | Rapid Adaptive Optical Recovery of Optimal Resolution over LargeVolumes |
title_short | Rapid Adaptive Optical Recovery of Optimal Resolution over LargeVolumes |
title_sort | rapid adaptive optical recovery of optimal resolution over largevolumes |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4069208/ https://www.ncbi.nlm.nih.gov/pubmed/24727653 http://dx.doi.org/10.1038/nmeth.2925 |
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