Rapid Adaptive Optical Recovery of Optimal Resolution over LargeVolumes

Using a de-scanned, laser-induced guide star and direct wavefront sensing, we demonstrate adaptive correction of complex optical aberrations at high numerical aperture and a 14 ms update rate. This permits us to compensate for the rapid spatial variation in aberration often encountered in biological...

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Detalles Bibliográficos
Autores principales: Wang, Kai, Milkie, Dan, Saxena, Ankur, Engerer, Peter, Misgeld, Thomas, Bronner, Marianne E., Mumm, Jeff, Betzig, Eric
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2014
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4069208/
https://www.ncbi.nlm.nih.gov/pubmed/24727653
http://dx.doi.org/10.1038/nmeth.2925
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author Wang, Kai
Milkie, Dan
Saxena, Ankur
Engerer, Peter
Misgeld, Thomas
Bronner, Marianne E.
Mumm, Jeff
Betzig, Eric
author_facet Wang, Kai
Milkie, Dan
Saxena, Ankur
Engerer, Peter
Misgeld, Thomas
Bronner, Marianne E.
Mumm, Jeff
Betzig, Eric
author_sort Wang, Kai
collection PubMed
description Using a de-scanned, laser-induced guide star and direct wavefront sensing, we demonstrate adaptive correction of complex optical aberrations at high numerical aperture and a 14 ms update rate. This permits us to compensate for the rapid spatial variation in aberration often encountered in biological specimens, and recover diffraction-limited imaging over large (> 240 μm)(3) volumes. We applied this to image fine neuronal processes and subcellular dynamics within the zebrafish brain.
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spelling pubmed-40692082014-12-01 Rapid Adaptive Optical Recovery of Optimal Resolution over LargeVolumes Wang, Kai Milkie, Dan Saxena, Ankur Engerer, Peter Misgeld, Thomas Bronner, Marianne E. Mumm, Jeff Betzig, Eric Nat Methods Article Using a de-scanned, laser-induced guide star and direct wavefront sensing, we demonstrate adaptive correction of complex optical aberrations at high numerical aperture and a 14 ms update rate. This permits us to compensate for the rapid spatial variation in aberration often encountered in biological specimens, and recover diffraction-limited imaging over large (> 240 μm)(3) volumes. We applied this to image fine neuronal processes and subcellular dynamics within the zebrafish brain. 2014-04-13 2014-06 /pmc/articles/PMC4069208/ /pubmed/24727653 http://dx.doi.org/10.1038/nmeth.2925 Text en http://www.nature.com/authors/editorial_policies/license.html#terms Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Wang, Kai
Milkie, Dan
Saxena, Ankur
Engerer, Peter
Misgeld, Thomas
Bronner, Marianne E.
Mumm, Jeff
Betzig, Eric
Rapid Adaptive Optical Recovery of Optimal Resolution over LargeVolumes
title Rapid Adaptive Optical Recovery of Optimal Resolution over LargeVolumes
title_full Rapid Adaptive Optical Recovery of Optimal Resolution over LargeVolumes
title_fullStr Rapid Adaptive Optical Recovery of Optimal Resolution over LargeVolumes
title_full_unstemmed Rapid Adaptive Optical Recovery of Optimal Resolution over LargeVolumes
title_short Rapid Adaptive Optical Recovery of Optimal Resolution over LargeVolumes
title_sort rapid adaptive optical recovery of optimal resolution over largevolumes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4069208/
https://www.ncbi.nlm.nih.gov/pubmed/24727653
http://dx.doi.org/10.1038/nmeth.2925
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