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Directed evolution of P-glycoprotein cysteines reveals site-specific, non-conservative substitutions that preserve multidrug resistance

Pgp (P-glycoprotein) is a prototype ABC (ATP-binding-cassette) transporter involved in multidrug resistance of cancer. We used directed evolution to replace six cytoplasmic Cys (cysteine) residues in Pgp with all 20 standard amino acids and selected for active mutants. From a pool of 75000 transform...

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Detalles Bibliográficos
Autores principales:	Swartz, Douglas J., Mok, Leo, Botta, Sri K., Singh, Anukriti, Altenberg, Guillermo A., Urbatsch, Ina L.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Portland Press Ltd. 2014
Materias:	Original Paper
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4069687/ https://www.ncbi.nlm.nih.gov/pubmed/24825346 http://dx.doi.org/10.1042/BSR20140062

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