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Analysis of age and gender associated N-glycoproteome in human whole saliva
BACKGROUND: Glycoproteins comprise a large portion of the salivary proteome and have great potential for biomarker discovery and disease diagnosis. However, the rate of production and the concentration of whole saliva change with age, gender and physiological states of the human body. Therefore, a t...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4070402/ https://www.ncbi.nlm.nih.gov/pubmed/24994967 http://dx.doi.org/10.1186/1559-0275-11-25 |
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author | Sun, Shisheng Zhao, Fei Wang, Qinzhe Zhong, Yaogang Cai, Tanxi Wu, Peng Yang, Fuquan Li, Zheng |
author_facet | Sun, Shisheng Zhao, Fei Wang, Qinzhe Zhong, Yaogang Cai, Tanxi Wu, Peng Yang, Fuquan Li, Zheng |
author_sort | Sun, Shisheng |
collection | PubMed |
description | BACKGROUND: Glycoproteins comprise a large portion of the salivary proteome and have great potential for biomarker discovery and disease diagnosis. However, the rate of production and the concentration of whole saliva change with age, gender and physiological states of the human body. Therefore, a thorough understanding of the salivary glycoproteome of healthy individuals of different ages and genders is a prerequisite for saliva to have clinical utility. METHODS: Formerly N-linked glycopeptides were isolated from the pooled whole saliva of six age and gender groups by hydrazide chemistry and hydrophilic affinity methods followed by mass spectrometry identification. Selected physiochemical characteristics of salivary glycoproteins were analyzed, and the salivary glycoproteomes of different age and gender groups were compared based on their glycoprotein components and gene ontology. RESULTS AND DISCUSSION: Among 85 N-glycoproteins identified in healthy human saliva, the majority were acidic proteins with low molecular weight. The numbers of salivary N-glycoproteins increased with age. Fifteen salivary glycoproteins were identified as potential age- or gender-associated glycoproteins, and many of them have functions related to innate immunity against microorganisms and oral cavity protection. Moreover, many salivary glycoproteins have been previously reported as disease related glycoproteins. This study reveals the important role of salivary glycoproteins in the maintenance of oral health and homeostasis and the great potential of saliva for biomarker discovery and disease diagnosis. |
format | Online Article Text |
id | pubmed-4070402 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Springer |
record_format | MEDLINE/PubMed |
spelling | pubmed-40704022014-07-03 Analysis of age and gender associated N-glycoproteome in human whole saliva Sun, Shisheng Zhao, Fei Wang, Qinzhe Zhong, Yaogang Cai, Tanxi Wu, Peng Yang, Fuquan Li, Zheng Clin Proteomics Research BACKGROUND: Glycoproteins comprise a large portion of the salivary proteome and have great potential for biomarker discovery and disease diagnosis. However, the rate of production and the concentration of whole saliva change with age, gender and physiological states of the human body. Therefore, a thorough understanding of the salivary glycoproteome of healthy individuals of different ages and genders is a prerequisite for saliva to have clinical utility. METHODS: Formerly N-linked glycopeptides were isolated from the pooled whole saliva of six age and gender groups by hydrazide chemistry and hydrophilic affinity methods followed by mass spectrometry identification. Selected physiochemical characteristics of salivary glycoproteins were analyzed, and the salivary glycoproteomes of different age and gender groups were compared based on their glycoprotein components and gene ontology. RESULTS AND DISCUSSION: Among 85 N-glycoproteins identified in healthy human saliva, the majority were acidic proteins with low molecular weight. The numbers of salivary N-glycoproteins increased with age. Fifteen salivary glycoproteins were identified as potential age- or gender-associated glycoproteins, and many of them have functions related to innate immunity against microorganisms and oral cavity protection. Moreover, many salivary glycoproteins have been previously reported as disease related glycoproteins. This study reveals the important role of salivary glycoproteins in the maintenance of oral health and homeostasis and the great potential of saliva for biomarker discovery and disease diagnosis. Springer 2014-06-05 /pmc/articles/PMC4070402/ /pubmed/24994967 http://dx.doi.org/10.1186/1559-0275-11-25 Text en Copyright © 2014 Sun et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Sun, Shisheng Zhao, Fei Wang, Qinzhe Zhong, Yaogang Cai, Tanxi Wu, Peng Yang, Fuquan Li, Zheng Analysis of age and gender associated N-glycoproteome in human whole saliva |
title | Analysis of age and gender associated N-glycoproteome in human whole saliva |
title_full | Analysis of age and gender associated N-glycoproteome in human whole saliva |
title_fullStr | Analysis of age and gender associated N-glycoproteome in human whole saliva |
title_full_unstemmed | Analysis of age and gender associated N-glycoproteome in human whole saliva |
title_short | Analysis of age and gender associated N-glycoproteome in human whole saliva |
title_sort | analysis of age and gender associated n-glycoproteome in human whole saliva |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4070402/ https://www.ncbi.nlm.nih.gov/pubmed/24994967 http://dx.doi.org/10.1186/1559-0275-11-25 |
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