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Bacterial Monitoring with Adhesive Sheet in the International Space Station-“Kibo”, the Japanese Experiment Module

Microbiological monitoring is important to assure microbiological safety, especially in long-duration space habitation. We have been continuously monitoring the abundance and diversity of bacteria in the International Space Station (ISS)-“Kibo” module to accumulate knowledge on microbes in the ISS....

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Autores principales: Ichijo, Tomoaki, Hieda, Hatsuki, Ishihara, Rie, Yamaguchi, Nobuyasu, Nasu, Masao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Japanese Society of Microbial Ecology/The Japanese Society of Soil Microbiology 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4070660/
https://www.ncbi.nlm.nih.gov/pubmed/23603802
http://dx.doi.org/10.1264/jsme2.ME12184
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author Ichijo, Tomoaki
Hieda, Hatsuki
Ishihara, Rie
Yamaguchi, Nobuyasu
Nasu, Masao
author_facet Ichijo, Tomoaki
Hieda, Hatsuki
Ishihara, Rie
Yamaguchi, Nobuyasu
Nasu, Masao
author_sort Ichijo, Tomoaki
collection PubMed
description Microbiological monitoring is important to assure microbiological safety, especially in long-duration space habitation. We have been continuously monitoring the abundance and diversity of bacteria in the International Space Station (ISS)-“Kibo” module to accumulate knowledge on microbes in the ISS. In this study, we used a new sampling device, a microbe-collecting adhesive sheet developed in our laboratory. This adhesive sheet has high operability, needs no water for sampling, and is easy to transport and store. We first validated the adhesive sheet as a sampling device to be used in a space habitat with regard to the stability of the bacterial number on the sheet during prolonged storage of up to 12 months. Bacterial abundance on the surfaces in Kibo was then determined and was lower than on the surfaces in our laboratory (10(5) cells [cm(2)](−1)), except for the return air grill, and the bacteria detected in Kibo were human skin microflora. From these aspects of microbial abundance and their phylogenetic affiliation, we concluded that Kibo has been microbiologically well maintained; however, microbial abundance may increase with the prolonged stay of astronauts. To ensure crew safety and understand bacterial dynamics in space habitation environments, continuous bacterial monitoring in Kibo is required.
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spelling pubmed-40706602014-07-24 Bacterial Monitoring with Adhesive Sheet in the International Space Station-“Kibo”, the Japanese Experiment Module Ichijo, Tomoaki Hieda, Hatsuki Ishihara, Rie Yamaguchi, Nobuyasu Nasu, Masao Microbes Environ Articles Microbiological monitoring is important to assure microbiological safety, especially in long-duration space habitation. We have been continuously monitoring the abundance and diversity of bacteria in the International Space Station (ISS)-“Kibo” module to accumulate knowledge on microbes in the ISS. In this study, we used a new sampling device, a microbe-collecting adhesive sheet developed in our laboratory. This adhesive sheet has high operability, needs no water for sampling, and is easy to transport and store. We first validated the adhesive sheet as a sampling device to be used in a space habitat with regard to the stability of the bacterial number on the sheet during prolonged storage of up to 12 months. Bacterial abundance on the surfaces in Kibo was then determined and was lower than on the surfaces in our laboratory (10(5) cells [cm(2)](−1)), except for the return air grill, and the bacteria detected in Kibo were human skin microflora. From these aspects of microbial abundance and their phylogenetic affiliation, we concluded that Kibo has been microbiologically well maintained; however, microbial abundance may increase with the prolonged stay of astronauts. To ensure crew safety and understand bacterial dynamics in space habitation environments, continuous bacterial monitoring in Kibo is required. Japanese Society of Microbial Ecology/The Japanese Society of Soil Microbiology 2013-06 2013-04-20 /pmc/articles/PMC4070660/ /pubmed/23603802 http://dx.doi.org/10.1264/jsme2.ME12184 Text en Copyright © 2013 by the Japanese Society of Microbial Ecology / the Japanese Society of Soil Microbiology http://creativecommons.org/licenses/by/3.0 This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Articles
Ichijo, Tomoaki
Hieda, Hatsuki
Ishihara, Rie
Yamaguchi, Nobuyasu
Nasu, Masao
Bacterial Monitoring with Adhesive Sheet in the International Space Station-“Kibo”, the Japanese Experiment Module
title Bacterial Monitoring with Adhesive Sheet in the International Space Station-“Kibo”, the Japanese Experiment Module
title_full Bacterial Monitoring with Adhesive Sheet in the International Space Station-“Kibo”, the Japanese Experiment Module
title_fullStr Bacterial Monitoring with Adhesive Sheet in the International Space Station-“Kibo”, the Japanese Experiment Module
title_full_unstemmed Bacterial Monitoring with Adhesive Sheet in the International Space Station-“Kibo”, the Japanese Experiment Module
title_short Bacterial Monitoring with Adhesive Sheet in the International Space Station-“Kibo”, the Japanese Experiment Module
title_sort bacterial monitoring with adhesive sheet in the international space station-“kibo”, the japanese experiment module
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4070660/
https://www.ncbi.nlm.nih.gov/pubmed/23603802
http://dx.doi.org/10.1264/jsme2.ME12184
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