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Design and Evaluation of PCR Primers for Denaturing Gradient Gel Electrophoresis Analysis of Plant Parasitic and Fungivorous Nematode Communities

A PCR-DGGE primer pair, Tyl2F-Tyl4R, specific to plant parasitic and fungivorous nematodes was designed based on the 18S rRNA gene. The results of community analysis using the primers showed that they are specific to the order Tylenchida. This primer pair detected species belonging to Tylenchida wit...

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Detalles Bibliográficos
Autor principal:	Kushida, Atsuhiko
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Japanese Society of Microbial Ecology/The Japanese Society of Soil Microbiology 2013
Materias:	Short Communication
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4070663/ https://www.ncbi.nlm.nih.gov/pubmed/23595022 http://dx.doi.org/10.1264/jsme2.ME12158

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