CARD-FISH for Environmental Microorganisms: Technical Advancement and Future Applications

Fluorescence in situ hybridization (FISH) has become a standard technique in environmental microbiology. More than 20 years have passed since this technique was first described, and it is currently used for the detection of ribosomal RNA, messenger RNA, and functional genes encoded on chromosomes. T...

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Detalles Bibliográficos
Autor principal: Kubota, Kengo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Japanese Society of Microbial Ecology/The Japanese Society of Soil Microbiology 2013
Materias:
Minireview
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4070690/
https://www.ncbi.nlm.nih.gov/pubmed/23124765
http://dx.doi.org/10.1264/jsme2.ME12107
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author Kubota, Kengo
author_facet Kubota, Kengo
author_sort Kubota, Kengo
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description Fluorescence in situ hybridization (FISH) has become a standard technique in environmental microbiology. More than 20 years have passed since this technique was first described, and it is currently used for the detection of ribosomal RNA, messenger RNA, and functional genes encoded on chromosomes. This review focuses on the advancement and applications of FISH combined with catalyzed reporter deposition (CARD, also known as tyramide signal amplification or TSA), in the detection of environmental microorganisms. Significant methodological improvements have been made in CARD-FISH technology, including its combination with other techniques and instruments.
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spelling pubmed-40706902014-07-24 CARD-FISH for Environmental Microorganisms: Technical Advancement and Future Applications Kubota, Kengo Microbes Environ Minireview Fluorescence in situ hybridization (FISH) has become a standard technique in environmental microbiology. More than 20 years have passed since this technique was first described, and it is currently used for the detection of ribosomal RNA, messenger RNA, and functional genes encoded on chromosomes. This review focuses on the advancement and applications of FISH combined with catalyzed reporter deposition (CARD, also known as tyramide signal amplification or TSA), in the detection of environmental microorganisms. Significant methodological improvements have been made in CARD-FISH technology, including its combination with other techniques and instruments. Japanese Society of Microbial Ecology/The Japanese Society of Soil Microbiology 2013-03 2012-10-31 /pmc/articles/PMC4070690/ /pubmed/23124765 http://dx.doi.org/10.1264/jsme2.ME12107 Text en Copyright © 2013 by the Japanese Society of Microbial Ecology / the Japanese Society of Soil Microbiology http://creativecommons.org/licenses/by/3.0 This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Minireview
Kubota, Kengo
CARD-FISH for Environmental Microorganisms: Technical Advancement and Future Applications
title CARD-FISH for Environmental Microorganisms: Technical Advancement and Future Applications
title_full CARD-FISH for Environmental Microorganisms: Technical Advancement and Future Applications
title_fullStr CARD-FISH for Environmental Microorganisms: Technical Advancement and Future Applications
title_full_unstemmed CARD-FISH for Environmental Microorganisms: Technical Advancement and Future Applications
title_short CARD-FISH for Environmental Microorganisms: Technical Advancement and Future Applications
title_sort card-fish for environmental microorganisms: technical advancement and future applications
topic Minireview
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4070690/
https://www.ncbi.nlm.nih.gov/pubmed/23124765
http://dx.doi.org/10.1264/jsme2.ME12107
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