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Uranium (U)-Tolerant Bacterial Diversity from U Ore Deposit of Domiasiat in North-East India and Its Prospective Utilisation in Bioremediation
Uranium (U)-tolerant aerobic chemo-heterotrophic bacteria were isolated from the sub-surface soils of U-rich deposits in Domiasiat, North East India. The bacterial community explored at molecular level by amplified ribosomal DNA restriction analysis (ARDRA) resulted in 51 distinct phylotypes. Bacter...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Japanese Society of Microbial Ecology/The Japanese Society of Soil Microbiology
2013
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4070695/ https://www.ncbi.nlm.nih.gov/pubmed/23080407 http://dx.doi.org/10.1264/jsme2.ME12074 |
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author | Kumar, Rakshak Nongkhlaw, Macmillan Acharya, Celin Joshi, Santa Ram |
author_facet | Kumar, Rakshak Nongkhlaw, Macmillan Acharya, Celin Joshi, Santa Ram |
author_sort | Kumar, Rakshak |
collection | PubMed |
description | Uranium (U)-tolerant aerobic chemo-heterotrophic bacteria were isolated from the sub-surface soils of U-rich deposits in Domiasiat, North East India. The bacterial community explored at molecular level by amplified ribosomal DNA restriction analysis (ARDRA) resulted in 51 distinct phylotypes. Bacterial community assemblages at the U mining site with the concentration of U ranging from 20 to 100 ppm, were found to be most diverse. Representative bacteria analysed by 16S rRNA gene sequencing were affiliated to Firmicutes (51%), Gammaproteobacteria (26%), Actinobacteria (11%), Bacteroidetes (10%) and Betaproteobacteria (2%). Representative strains removed more than 90% and 53% of U from 100 μM and 2 mM uranyl nitrate solutions, respectively, at pH 3.5 within 10 min of exposure and the activity was retained until 24 h. Overall, 76% of characterized isolates possessed phosphatase enzyme and 53% had P(IB)-type ATPase genes. This study generated baseline information on the diverse indigenous U-tolerant bacteria which could serve as an indicator to estimate the environmental impact expected to be caused by mining in the future. Also, these natural isolates efficient in uranium binding and harbouring phosphatase enzyme and metal-transporting genes could possibly play a vital role in the bioremediation of metal-/radionuclide-contaminated environments. |
format | Online Article Text |
id | pubmed-4070695 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Japanese Society of Microbial Ecology/The Japanese Society of Soil Microbiology |
record_format | MEDLINE/PubMed |
spelling | pubmed-40706952014-07-24 Uranium (U)-Tolerant Bacterial Diversity from U Ore Deposit of Domiasiat in North-East India and Its Prospective Utilisation in Bioremediation Kumar, Rakshak Nongkhlaw, Macmillan Acharya, Celin Joshi, Santa Ram Microbes Environ Articles Uranium (U)-tolerant aerobic chemo-heterotrophic bacteria were isolated from the sub-surface soils of U-rich deposits in Domiasiat, North East India. The bacterial community explored at molecular level by amplified ribosomal DNA restriction analysis (ARDRA) resulted in 51 distinct phylotypes. Bacterial community assemblages at the U mining site with the concentration of U ranging from 20 to 100 ppm, were found to be most diverse. Representative bacteria analysed by 16S rRNA gene sequencing were affiliated to Firmicutes (51%), Gammaproteobacteria (26%), Actinobacteria (11%), Bacteroidetes (10%) and Betaproteobacteria (2%). Representative strains removed more than 90% and 53% of U from 100 μM and 2 mM uranyl nitrate solutions, respectively, at pH 3.5 within 10 min of exposure and the activity was retained until 24 h. Overall, 76% of characterized isolates possessed phosphatase enzyme and 53% had P(IB)-type ATPase genes. This study generated baseline information on the diverse indigenous U-tolerant bacteria which could serve as an indicator to estimate the environmental impact expected to be caused by mining in the future. Also, these natural isolates efficient in uranium binding and harbouring phosphatase enzyme and metal-transporting genes could possibly play a vital role in the bioremediation of metal-/radionuclide-contaminated environments. Japanese Society of Microbial Ecology/The Japanese Society of Soil Microbiology 2013-03 2012-10-19 /pmc/articles/PMC4070695/ /pubmed/23080407 http://dx.doi.org/10.1264/jsme2.ME12074 Text en Copyright © 2013 by the Japanese Society of Microbial Ecology / the Japanese Society of Soil Microbiology http://creativecommons.org/licenses/by/3.0 This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Articles Kumar, Rakshak Nongkhlaw, Macmillan Acharya, Celin Joshi, Santa Ram Uranium (U)-Tolerant Bacterial Diversity from U Ore Deposit of Domiasiat in North-East India and Its Prospective Utilisation in Bioremediation |
title | Uranium (U)-Tolerant Bacterial Diversity from U Ore Deposit of Domiasiat in North-East India and Its Prospective Utilisation in Bioremediation |
title_full | Uranium (U)-Tolerant Bacterial Diversity from U Ore Deposit of Domiasiat in North-East India and Its Prospective Utilisation in Bioremediation |
title_fullStr | Uranium (U)-Tolerant Bacterial Diversity from U Ore Deposit of Domiasiat in North-East India and Its Prospective Utilisation in Bioremediation |
title_full_unstemmed | Uranium (U)-Tolerant Bacterial Diversity from U Ore Deposit of Domiasiat in North-East India and Its Prospective Utilisation in Bioremediation |
title_short | Uranium (U)-Tolerant Bacterial Diversity from U Ore Deposit of Domiasiat in North-East India and Its Prospective Utilisation in Bioremediation |
title_sort | uranium (u)-tolerant bacterial diversity from u ore deposit of domiasiat in north-east india and its prospective utilisation in bioremediation |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4070695/ https://www.ncbi.nlm.nih.gov/pubmed/23080407 http://dx.doi.org/10.1264/jsme2.ME12074 |
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