Excystment-Dependent Alteration of Protein Expression in Terrestrial Ciliate Colpoda cucullus

Protein expression during the excystment of Colpoda cucullus was studied by SDS-PAGE. The expression levels of 60-, 50- and 49-kDa proteins were markedly changed from the early to later stage of excystment. The 60-kDa protein (p60) was temporarily expressed first, and its expression was inhibited by...

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Detalles Bibliográficos
Autores principales: Sogame, Yoichiro, Kojima, Katsuhiko, Takeshita, Toshikazu, Kinoshita, Eiji, Funadani, Ryoji, Matsuoka, Tatsuomi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Japanese Society of Microbial Ecology/The Japanese Society of Soil Microbiology 2013
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4070955/
https://www.ncbi.nlm.nih.gov/pubmed/23628864
http://dx.doi.org/10.1264/jsme2.ME12200
Descripción
Sumario:Protein expression during the excystment of Colpoda cucullus was studied by SDS-PAGE. The expression levels of 60-, 50- and 49-kDa proteins were markedly changed from the early to later stage of excystment. The 60-kDa protein (p60) was temporarily expressed first, and its expression was inhibited by actinomycin D. LC-MS/MS analysis showed that the amino acid sequences of p60 partially coincided with those of the Paramecium tetraurelia unnamed protein homologous to DEAD-box RNA helicase. These results suggest that p60 expression is enhanced by transcriptional regulation and may be involved in initiating the molecular events leading to cellular morphogenesis.

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