Excystment-Dependent Alteration of Protein Expression in Terrestrial Ciliate Colpoda cucullus

Protein expression during the excystment of Colpoda cucullus was studied by SDS-PAGE. The expression levels of 60-, 50- and 49-kDa proteins were markedly changed from the early to later stage of excystment. The 60-kDa protein (p60) was temporarily expressed first, and its expression was inhibited by...

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Autores principales: Sogame, Yoichiro, Kojima, Katsuhiko, Takeshita, Toshikazu, Kinoshita, Eiji, Funadani, Ryoji, Matsuoka, Tatsuomi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Japanese Society of Microbial Ecology/The Japanese Society of Soil Microbiology 2013
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4070955/
https://www.ncbi.nlm.nih.gov/pubmed/23628864
http://dx.doi.org/10.1264/jsme2.ME12200
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author Sogame, Yoichiro
Kojima, Katsuhiko
Takeshita, Toshikazu
Kinoshita, Eiji
Funadani, Ryoji
Matsuoka, Tatsuomi
author_facet Sogame, Yoichiro
Kojima, Katsuhiko
Takeshita, Toshikazu
Kinoshita, Eiji
Funadani, Ryoji
Matsuoka, Tatsuomi
author_sort Sogame, Yoichiro
collection PubMed
description Protein expression during the excystment of Colpoda cucullus was studied by SDS-PAGE. The expression levels of 60-, 50- and 49-kDa proteins were markedly changed from the early to later stage of excystment. The 60-kDa protein (p60) was temporarily expressed first, and its expression was inhibited by actinomycin D. LC-MS/MS analysis showed that the amino acid sequences of p60 partially coincided with those of the Paramecium tetraurelia unnamed protein homologous to DEAD-box RNA helicase. These results suggest that p60 expression is enhanced by transcriptional regulation and may be involved in initiating the molecular events leading to cellular morphogenesis.
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spelling pubmed-40709552014-07-24 Excystment-Dependent Alteration of Protein Expression in Terrestrial Ciliate Colpoda cucullus Sogame, Yoichiro Kojima, Katsuhiko Takeshita, Toshikazu Kinoshita, Eiji Funadani, Ryoji Matsuoka, Tatsuomi Microbes Environ Short Communication Protein expression during the excystment of Colpoda cucullus was studied by SDS-PAGE. The expression levels of 60-, 50- and 49-kDa proteins were markedly changed from the early to later stage of excystment. The 60-kDa protein (p60) was temporarily expressed first, and its expression was inhibited by actinomycin D. LC-MS/MS analysis showed that the amino acid sequences of p60 partially coincided with those of the Paramecium tetraurelia unnamed protein homologous to DEAD-box RNA helicase. These results suggest that p60 expression is enhanced by transcriptional regulation and may be involved in initiating the molecular events leading to cellular morphogenesis. Japanese Society of Microbial Ecology/The Japanese Society of Soil Microbiology 2013-09 2013-04-26 /pmc/articles/PMC4070955/ /pubmed/23628864 http://dx.doi.org/10.1264/jsme2.ME12200 Text en Copyright © 2013 by the Japanese Society of Microbial Ecology / the Japanese Society of Soil Microbiology http://creativecommons.org/licenses/by/3.0 This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Short Communication
Sogame, Yoichiro
Kojima, Katsuhiko
Takeshita, Toshikazu
Kinoshita, Eiji
Funadani, Ryoji
Matsuoka, Tatsuomi
Excystment-Dependent Alteration of Protein Expression in Terrestrial Ciliate Colpoda cucullus
title Excystment-Dependent Alteration of Protein Expression in Terrestrial Ciliate Colpoda cucullus
title_full Excystment-Dependent Alteration of Protein Expression in Terrestrial Ciliate Colpoda cucullus
title_fullStr Excystment-Dependent Alteration of Protein Expression in Terrestrial Ciliate Colpoda cucullus
title_full_unstemmed Excystment-Dependent Alteration of Protein Expression in Terrestrial Ciliate Colpoda cucullus
title_short Excystment-Dependent Alteration of Protein Expression in Terrestrial Ciliate Colpoda cucullus
title_sort excystment-dependent alteration of protein expression in terrestrial ciliate colpoda cucullus
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4070955/
https://www.ncbi.nlm.nih.gov/pubmed/23628864
http://dx.doi.org/10.1264/jsme2.ME12200
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