Cargando…

Comparison of the Digene HPV Genotyping LQ Test and the PANArray HPV Genotyping Chip for Detection of High-Risk or Probable High-Risk Human Papillomavirus Genotypes

BACKGROUND: We evaluated the performance of two different array-based techniques, a bead-based multiplex genotyping method (LQ; digene HPV Genotyping LQ Test, QIAGEN, Germany) and a DNA chip-based method using peptide nucleic acid probes (PANArray; PANArray HPV Genotyping Chip, Panagene, Korea), for...

Descripción completa

Detalles Bibliográficos
Autores principales: Park, Kyung Sun, Kim, Ji-Youn, Ki, Chang-Seok, Lee, Nam Yong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Society for Laboratory Medicine 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4071184/
https://www.ncbi.nlm.nih.gov/pubmed/24982832
http://dx.doi.org/10.3343/alm.2014.34.4.279
_version_ 1782322783042142208
author Park, Kyung Sun
Kim, Ji-Youn
Ki, Chang-Seok
Lee, Nam Yong
author_facet Park, Kyung Sun
Kim, Ji-Youn
Ki, Chang-Seok
Lee, Nam Yong
author_sort Park, Kyung Sun
collection PubMed
description BACKGROUND: We evaluated the performance of two different array-based techniques, a bead-based multiplex genotyping method (LQ; digene HPV Genotyping LQ Test, QIAGEN, Germany) and a DNA chip-based method using peptide nucleic acid probes (PANArray; PANArray HPV Genotyping Chip, Panagene, Korea), for detection of human papillomavirus (HPV) and genotyping of high-risk (HR) or probable high-risk (PHR) HPVs in healthy patients who visited a health-promotion center. METHODS: We obtained 508 unselected, consecutive cervicovaginal swab specimens. All specimens were examined by using the PANArray and LQ tests. All HPV-positive samples were then analyzed by multiplex PCR and direct sequencing. RESULTS: The LQ test detected 47 HPV-positive cases (9.3%) with HR or PHR genotypes and the PANArray test identified 36 cases (7.1%). When the results of LQ and PANArray were compared by using comprehensive genotyping (integrated interpretation of the results of LQ, PANArray, multiplex PCR, and direct sequencing) for the detection of HR or PHR genotypes, the kappa values were 0.44 and 0.30 for LQ and PANArray, respectively. In comparison to comprehensive genotyping, the LQ test yielded 53 (60.0%) concordant and 12 (13.5%) compatible results, and the PANArray yielded 36 (40.4%) concordant and three (3.4%) compatible results. CONCLUSIONS: The results of the LQ test had higher concordance and/or greater compatibility with those of comprehensive genotyping for the detection of HR or PHR genotypes than those of the PANArray test.
format Online
Article
Text
id pubmed-4071184
institution National Center for Biotechnology Information
language English
publishDate 2014
publisher The Korean Society for Laboratory Medicine
record_format MEDLINE/PubMed
spelling pubmed-40711842014-07-01 Comparison of the Digene HPV Genotyping LQ Test and the PANArray HPV Genotyping Chip for Detection of High-Risk or Probable High-Risk Human Papillomavirus Genotypes Park, Kyung Sun Kim, Ji-Youn Ki, Chang-Seok Lee, Nam Yong Ann Lab Med Original Article BACKGROUND: We evaluated the performance of two different array-based techniques, a bead-based multiplex genotyping method (LQ; digene HPV Genotyping LQ Test, QIAGEN, Germany) and a DNA chip-based method using peptide nucleic acid probes (PANArray; PANArray HPV Genotyping Chip, Panagene, Korea), for detection of human papillomavirus (HPV) and genotyping of high-risk (HR) or probable high-risk (PHR) HPVs in healthy patients who visited a health-promotion center. METHODS: We obtained 508 unselected, consecutive cervicovaginal swab specimens. All specimens were examined by using the PANArray and LQ tests. All HPV-positive samples were then analyzed by multiplex PCR and direct sequencing. RESULTS: The LQ test detected 47 HPV-positive cases (9.3%) with HR or PHR genotypes and the PANArray test identified 36 cases (7.1%). When the results of LQ and PANArray were compared by using comprehensive genotyping (integrated interpretation of the results of LQ, PANArray, multiplex PCR, and direct sequencing) for the detection of HR or PHR genotypes, the kappa values were 0.44 and 0.30 for LQ and PANArray, respectively. In comparison to comprehensive genotyping, the LQ test yielded 53 (60.0%) concordant and 12 (13.5%) compatible results, and the PANArray yielded 36 (40.4%) concordant and three (3.4%) compatible results. CONCLUSIONS: The results of the LQ test had higher concordance and/or greater compatibility with those of comprehensive genotyping for the detection of HR or PHR genotypes than those of the PANArray test. The Korean Society for Laboratory Medicine 2014-07 2014-06-19 /pmc/articles/PMC4071184/ /pubmed/24982832 http://dx.doi.org/10.3343/alm.2014.34.4.279 Text en © The Korean Society for Laboratory Medicine. http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Park, Kyung Sun
Kim, Ji-Youn
Ki, Chang-Seok
Lee, Nam Yong
Comparison of the Digene HPV Genotyping LQ Test and the PANArray HPV Genotyping Chip for Detection of High-Risk or Probable High-Risk Human Papillomavirus Genotypes
title Comparison of the Digene HPV Genotyping LQ Test and the PANArray HPV Genotyping Chip for Detection of High-Risk or Probable High-Risk Human Papillomavirus Genotypes
title_full Comparison of the Digene HPV Genotyping LQ Test and the PANArray HPV Genotyping Chip for Detection of High-Risk or Probable High-Risk Human Papillomavirus Genotypes
title_fullStr Comparison of the Digene HPV Genotyping LQ Test and the PANArray HPV Genotyping Chip for Detection of High-Risk or Probable High-Risk Human Papillomavirus Genotypes
title_full_unstemmed Comparison of the Digene HPV Genotyping LQ Test and the PANArray HPV Genotyping Chip for Detection of High-Risk or Probable High-Risk Human Papillomavirus Genotypes
title_short Comparison of the Digene HPV Genotyping LQ Test and the PANArray HPV Genotyping Chip for Detection of High-Risk or Probable High-Risk Human Papillomavirus Genotypes
title_sort comparison of the digene hpv genotyping lq test and the panarray hpv genotyping chip for detection of high-risk or probable high-risk human papillomavirus genotypes
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4071184/
https://www.ncbi.nlm.nih.gov/pubmed/24982832
http://dx.doi.org/10.3343/alm.2014.34.4.279
work_keys_str_mv AT parkkyungsun comparisonofthedigenehpvgenotypinglqtestandthepanarrayhpvgenotypingchipfordetectionofhighriskorprobablehighriskhumanpapillomavirusgenotypes
AT kimjiyoun comparisonofthedigenehpvgenotypinglqtestandthepanarrayhpvgenotypingchipfordetectionofhighriskorprobablehighriskhumanpapillomavirusgenotypes
AT kichangseok comparisonofthedigenehpvgenotypinglqtestandthepanarrayhpvgenotypingchipfordetectionofhighriskorprobablehighriskhumanpapillomavirusgenotypes
AT leenamyong comparisonofthedigenehpvgenotypinglqtestandthepanarrayhpvgenotypingchipfordetectionofhighriskorprobablehighriskhumanpapillomavirusgenotypes