Reduced quenching and extraction time for mammalian cells using filtration and syringe extraction

In order to preserve the in vivo metabolite levels of cells, a quenching protocol must be quickly executed to avoid degradation of labile metabolites either chemically or biologically. In the case of mammalian cell cultures cultivated in complex media, a wash step previous to quenching is necessary...

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Detalles Bibliográficos
Autores principales: Hernández Bort, Juan A., Shanmukam, Vinoth, Pabst, Martin, Windwarder, Markus, Neumann, Laura, Alchalabi, Ali, Krebiehl, Guido, Koellensperger, Gunda, Hann, Stephan, Sonntag, Denise, Altmann, Friedrich, Heel, Christine, Borth, Nicole
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier Science Publishers 2014
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4071440/
https://www.ncbi.nlm.nih.gov/pubmed/24794799
http://dx.doi.org/10.1016/j.jbiotec.2014.04.014

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