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Development of an experimental diet model in rats to study hyperlipidemia and insulin resistance, markers for coronary heart disease
OBJECTIVES: The objective of this study is to develop an experimental model of hyperlipidemia and insulin resistance (IR), markers of coronary heart disease (CHD) using high fat and high sugar (HFHS) diet and to evaluate the efficacy of the model using atorvastatin, a known antihyperlipidemic drug,...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Medknow Publications & Media Pvt Ltd
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4071702/ https://www.ncbi.nlm.nih.gov/pubmed/24987172 http://dx.doi.org/10.4103/0253-7613.132156 |
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author | Munshi, Renuka P. Joshi, Samidha G. Rane, Bhagyeshri N. |
author_facet | Munshi, Renuka P. Joshi, Samidha G. Rane, Bhagyeshri N. |
author_sort | Munshi, Renuka P. |
collection | PubMed |
description | OBJECTIVES: The objective of this study is to develop an experimental model of hyperlipidemia and insulin resistance (IR), markers of coronary heart disease (CHD) using high fat and high sugar (HFHS) diet and to evaluate the efficacy of the model using atorvastatin, a known antihyperlipidemic drug, pioglitazone, a known insulin sensitizer, and Tinospora cordifolia (Tc), an antidiabetic plant. MATERIALS AND METHODS: Following Institutional Animal Ethics Committee permission, the study was conducted in male Wistar rats (200-270 g). The model was developed using a high fat (vanaspati ghee: coconut oil, 3:1) oral diet along with 25% fructose (high sugar) added in drinking water over a period of 6 weeks. Atorvastatin (2.1 mg/kg/day), pioglitazone (2.7 mg/kg/day) and Tc (200 mg/kg/day) were administered 3 weeks after initiation of HFHS diet and continued for another 3 weeks. Parameters assessed were weight, lipid profile, fasting blood glucose, insulin, and gastric emptying. Serum malondialdehyde (MDA) and catalase were assessed as markers of oxidative stress. RESULTS: Administration of HFHS diet demonstrated a significant increase in blood glucose, insulin, total and low density lipoprotein cholesterol and triglycerides with a decrease in high density lipoprotein cholesterol. Treatment with test drugs decreased blood sugar, insulin, lipid parameters, increased gastric emptying rate, decreased MDA levels, and catalase activity when compared to HFHS diet group, confirming the efficacy of the model. Atherogenic index of all the test drugs (0.48, 0.57, and 0.53) was significantly lower as compared to HFHS diet group (1.107). CONCLUSION: This study confirms the development of a diet based cost-effective and time efficient experimental model, which can be used to study two important markers of cardiovascular disease that is, hyperlipidemia and IR and to explore the efficacy of new molecules in CHD. |
format | Online Article Text |
id | pubmed-4071702 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Medknow Publications & Media Pvt Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-40717022014-07-01 Development of an experimental diet model in rats to study hyperlipidemia and insulin resistance, markers for coronary heart disease Munshi, Renuka P. Joshi, Samidha G. Rane, Bhagyeshri N. Indian J Pharmacol Research Article OBJECTIVES: The objective of this study is to develop an experimental model of hyperlipidemia and insulin resistance (IR), markers of coronary heart disease (CHD) using high fat and high sugar (HFHS) diet and to evaluate the efficacy of the model using atorvastatin, a known antihyperlipidemic drug, pioglitazone, a known insulin sensitizer, and Tinospora cordifolia (Tc), an antidiabetic plant. MATERIALS AND METHODS: Following Institutional Animal Ethics Committee permission, the study was conducted in male Wistar rats (200-270 g). The model was developed using a high fat (vanaspati ghee: coconut oil, 3:1) oral diet along with 25% fructose (high sugar) added in drinking water over a period of 6 weeks. Atorvastatin (2.1 mg/kg/day), pioglitazone (2.7 mg/kg/day) and Tc (200 mg/kg/day) were administered 3 weeks after initiation of HFHS diet and continued for another 3 weeks. Parameters assessed were weight, lipid profile, fasting blood glucose, insulin, and gastric emptying. Serum malondialdehyde (MDA) and catalase were assessed as markers of oxidative stress. RESULTS: Administration of HFHS diet demonstrated a significant increase in blood glucose, insulin, total and low density lipoprotein cholesterol and triglycerides with a decrease in high density lipoprotein cholesterol. Treatment with test drugs decreased blood sugar, insulin, lipid parameters, increased gastric emptying rate, decreased MDA levels, and catalase activity when compared to HFHS diet group, confirming the efficacy of the model. Atherogenic index of all the test drugs (0.48, 0.57, and 0.53) was significantly lower as compared to HFHS diet group (1.107). CONCLUSION: This study confirms the development of a diet based cost-effective and time efficient experimental model, which can be used to study two important markers of cardiovascular disease that is, hyperlipidemia and IR and to explore the efficacy of new molecules in CHD. Medknow Publications & Media Pvt Ltd 2014 /pmc/articles/PMC4071702/ /pubmed/24987172 http://dx.doi.org/10.4103/0253-7613.132156 Text en Copyright: © Indian Journal of Pharmacology http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Munshi, Renuka P. Joshi, Samidha G. Rane, Bhagyeshri N. Development of an experimental diet model in rats to study hyperlipidemia and insulin resistance, markers for coronary heart disease |
title | Development of an experimental diet model in rats to study hyperlipidemia and insulin resistance, markers for coronary heart disease |
title_full | Development of an experimental diet model in rats to study hyperlipidemia and insulin resistance, markers for coronary heart disease |
title_fullStr | Development of an experimental diet model in rats to study hyperlipidemia and insulin resistance, markers for coronary heart disease |
title_full_unstemmed | Development of an experimental diet model in rats to study hyperlipidemia and insulin resistance, markers for coronary heart disease |
title_short | Development of an experimental diet model in rats to study hyperlipidemia and insulin resistance, markers for coronary heart disease |
title_sort | development of an experimental diet model in rats to study hyperlipidemia and insulin resistance, markers for coronary heart disease |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4071702/ https://www.ncbi.nlm.nih.gov/pubmed/24987172 http://dx.doi.org/10.4103/0253-7613.132156 |
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