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SPX1 is an important component in the phosphorus signalling network of common bean regulating root growth and phosphorus homeostasis
Proteins containing the SPX domain are believed to play vital roles in the phosphorus (P) signalling network in plants. However, the functions of SPX proteins in legumes remain largely unknown. In this study, three SPX members, PvSPX1–PvSPX3 were cloned from common bean (Phaseolus vulgaris L.). It w...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4071846/ https://www.ncbi.nlm.nih.gov/pubmed/24790114 http://dx.doi.org/10.1093/jxb/eru183 |
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author | Yao, Zhu-Fang Liang, Cui-Yue Zhang, Qing Chen, Zhi-Jian Xiao, Bi-Xian Tian, Jiang Liao, Hong |
author_facet | Yao, Zhu-Fang Liang, Cui-Yue Zhang, Qing Chen, Zhi-Jian Xiao, Bi-Xian Tian, Jiang Liao, Hong |
author_sort | Yao, Zhu-Fang |
collection | PubMed |
description | Proteins containing the SPX domain are believed to play vital roles in the phosphorus (P) signalling network in plants. However, the functions of SPX proteins in legumes remain largely unknown. In this study, three SPX members, PvSPX1–PvSPX3 were cloned from common bean (Phaseolus vulgaris L.). It was found that the transcripts of all three PvSPX members were significantly enhanced in both bean leaves and roots by phosphate (Pi) starvation. Among them, the expression of nuclear localized PvSPX1 showed more sensitive and rapid responses to Pi starvation. Consistently, only overexpression of PvSPX1 resulted in increased root P concentration and modified morphology of transgenic bean hairy roots, such as inhibited root growth and an enlarged root hair zone. It was further demonstrated that PvSPX1 transcripts were up-regulated by overexpressing PvPHR1, and overexpressing PvSPX1 led to increased transcripts of 10 Pi starvation-responsive genes in transgenic bean hairy roots. Taken together, it is suggested that PvSPX1 is a positive regulator in the P signalling network of common bean, and is downstream of PvPHR1. |
format | Online Article Text |
id | pubmed-4071846 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-40718462014-06-26 SPX1 is an important component in the phosphorus signalling network of common bean regulating root growth and phosphorus homeostasis Yao, Zhu-Fang Liang, Cui-Yue Zhang, Qing Chen, Zhi-Jian Xiao, Bi-Xian Tian, Jiang Liao, Hong J Exp Bot Research Paper Proteins containing the SPX domain are believed to play vital roles in the phosphorus (P) signalling network in plants. However, the functions of SPX proteins in legumes remain largely unknown. In this study, three SPX members, PvSPX1–PvSPX3 were cloned from common bean (Phaseolus vulgaris L.). It was found that the transcripts of all three PvSPX members were significantly enhanced in both bean leaves and roots by phosphate (Pi) starvation. Among them, the expression of nuclear localized PvSPX1 showed more sensitive and rapid responses to Pi starvation. Consistently, only overexpression of PvSPX1 resulted in increased root P concentration and modified morphology of transgenic bean hairy roots, such as inhibited root growth and an enlarged root hair zone. It was further demonstrated that PvSPX1 transcripts were up-regulated by overexpressing PvPHR1, and overexpressing PvSPX1 led to increased transcripts of 10 Pi starvation-responsive genes in transgenic bean hairy roots. Taken together, it is suggested that PvSPX1 is a positive regulator in the P signalling network of common bean, and is downstream of PvPHR1. Oxford University Press 2014-07 2014-04-30 /pmc/articles/PMC4071846/ /pubmed/24790114 http://dx.doi.org/10.1093/jxb/eru183 Text en © The Author 2014. Published by Oxford University Press on behalf of the Society for Experimental Biology. http://creativecommons.org/licenses/by/3.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Paper Yao, Zhu-Fang Liang, Cui-Yue Zhang, Qing Chen, Zhi-Jian Xiao, Bi-Xian Tian, Jiang Liao, Hong SPX1 is an important component in the phosphorus signalling network of common bean regulating root growth and phosphorus homeostasis |
title |
SPX1 is an important component in the phosphorus signalling network of common bean regulating root growth and phosphorus homeostasis |
title_full |
SPX1 is an important component in the phosphorus signalling network of common bean regulating root growth and phosphorus homeostasis |
title_fullStr |
SPX1 is an important component in the phosphorus signalling network of common bean regulating root growth and phosphorus homeostasis |
title_full_unstemmed |
SPX1 is an important component in the phosphorus signalling network of common bean regulating root growth and phosphorus homeostasis |
title_short |
SPX1 is an important component in the phosphorus signalling network of common bean regulating root growth and phosphorus homeostasis |
title_sort | spx1 is an important component in the phosphorus signalling network of common bean regulating root growth and phosphorus homeostasis |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4071846/ https://www.ncbi.nlm.nih.gov/pubmed/24790114 http://dx.doi.org/10.1093/jxb/eru183 |
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