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Real-Time Assay as A Tool for Detecting lytA Gene in Streptococcus pneumoniae Isolates
OBJECTIVE: In-time diagnosis of Streptococcus pneumoniae (S. pneumonia) can play a significant role in decreasing morbidity and mortality rate. Applying molecular methods has gained popularity due to the existing limits of routine diagnostic methods. Examining the expression of different genes of th...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Royan Institute
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4072081/ https://www.ncbi.nlm.nih.gov/pubmed/24567943 |
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author | Hajia, Masoud Farzanehkhah, Mohammad Hajihashemi, Bahareh Dolatyar, AliReza Imani, Mohsen Saburian, Roghieh Rahnamaye Farzami, Marjan Rahbar, Mohammad |
author_facet | Hajia, Masoud Farzanehkhah, Mohammad Hajihashemi, Bahareh Dolatyar, AliReza Imani, Mohsen Saburian, Roghieh Rahnamaye Farzami, Marjan Rahbar, Mohammad |
author_sort | Hajia, Masoud |
collection | PubMed |
description | OBJECTIVE: In-time diagnosis of Streptococcus pneumoniae (S. pneumonia) can play a significant role in decreasing morbidity and mortality rate. Applying molecular methods has gained popularity due to the existing limits of routine diagnostic methods. Examining the expression of different genes of this bacterium through different molecular methods suggests that lytA gene has a higher sensitivity and specificity in diagnosis of Streptococcus pneumoniae. The aim of this study was to evalutate lytA gene expression in diagnosis of invasive S. pneumonia in culture positive specimens by real-time polymerase chain reaction (PCR). MATERIALS AND METHODS: IIn this a descriptive study, All received specimens were isolated to identify S. pneumoniae. DNA was then extracted and after optimizing the test and determining the detection limit, samples were tested by real-time PCR using lytA gene primers. RESULTS: Twenty seven isolates were diagnosed as S. pneumoniae. In all, the extracted DNA was positive in real-time method. The electrophoresis of the products also confirmed the presence of single product b along with the 53 base pair fragment. The detection limit of the test was less 6 colony forming unit (CFU). CONCLUSION: Real-Time PCR seems to provide reliable and rapid results. We suggest that this test should be conducted on the preliminary isolated specimens, since applying various biochemical tests need one extra working day. |
format | Online Article Text |
id | pubmed-4072081 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Royan Institute |
record_format | MEDLINE/PubMed |
spelling | pubmed-40720812014-07-09 Real-Time Assay as A Tool for Detecting lytA Gene in Streptococcus pneumoniae Isolates Hajia, Masoud Farzanehkhah, Mohammad Hajihashemi, Bahareh Dolatyar, AliReza Imani, Mohsen Saburian, Roghieh Rahnamaye Farzami, Marjan Rahbar, Mohammad Cell J Original Article OBJECTIVE: In-time diagnosis of Streptococcus pneumoniae (S. pneumonia) can play a significant role in decreasing morbidity and mortality rate. Applying molecular methods has gained popularity due to the existing limits of routine diagnostic methods. Examining the expression of different genes of this bacterium through different molecular methods suggests that lytA gene has a higher sensitivity and specificity in diagnosis of Streptococcus pneumoniae. The aim of this study was to evalutate lytA gene expression in diagnosis of invasive S. pneumonia in culture positive specimens by real-time polymerase chain reaction (PCR). MATERIALS AND METHODS: IIn this a descriptive study, All received specimens were isolated to identify S. pneumoniae. DNA was then extracted and after optimizing the test and determining the detection limit, samples were tested by real-time PCR using lytA gene primers. RESULTS: Twenty seven isolates were diagnosed as S. pneumoniae. In all, the extracted DNA was positive in real-time method. The electrophoresis of the products also confirmed the presence of single product b along with the 53 base pair fragment. The detection limit of the test was less 6 colony forming unit (CFU). CONCLUSION: Real-Time PCR seems to provide reliable and rapid results. We suggest that this test should be conducted on the preliminary isolated specimens, since applying various biochemical tests need one extra working day. Royan Institute 2014 2014-05-25 /pmc/articles/PMC4072081/ /pubmed/24567943 Text en Any use, distribution, reproduction or abstract of this publication in any medium, with the exception of commercial purposes, is permitted provided the original work is properly cited http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Hajia, Masoud Farzanehkhah, Mohammad Hajihashemi, Bahareh Dolatyar, AliReza Imani, Mohsen Saburian, Roghieh Rahnamaye Farzami, Marjan Rahbar, Mohammad Real-Time Assay as A Tool for Detecting lytA Gene in Streptococcus pneumoniae Isolates |
title | Real-Time Assay as A Tool for Detecting lytA Gene
in Streptococcus pneumoniae Isolates |
title_full | Real-Time Assay as A Tool for Detecting lytA Gene
in Streptococcus pneumoniae Isolates |
title_fullStr | Real-Time Assay as A Tool for Detecting lytA Gene
in Streptococcus pneumoniae Isolates |
title_full_unstemmed | Real-Time Assay as A Tool for Detecting lytA Gene
in Streptococcus pneumoniae Isolates |
title_short | Real-Time Assay as A Tool for Detecting lytA Gene
in Streptococcus pneumoniae Isolates |
title_sort | real-time assay as a tool for detecting lyta gene
in streptococcus pneumoniae isolates |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4072081/ https://www.ncbi.nlm.nih.gov/pubmed/24567943 |
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