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Ultra-High Throughput Synthesis of Nanoparticles with Homogeneous Size Distribution Using a Coaxial Turbulent Jet Mixer
[Image: see text] High-throughput production of nanoparticles (NPs) with controlled quality is critical for their clinical translation into effective nanomedicines for diagnostics and therapeutics. Here we report a simple and versatile coaxial turbulent jet mixer that can synthesize a variety of NPs...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Chemical Society
2014
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4072409/ https://www.ncbi.nlm.nih.gov/pubmed/24824296 http://dx.doi.org/10.1021/nn501371n |
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author | Lim, Jong-Min Swami, Archana Gilson, Laura M. Chopra, Sunandini Choi, Sungyoung Wu, Jun Langer, Robert Karnik, Rohit Farokhzad, Omid C. |
author_facet | Lim, Jong-Min Swami, Archana Gilson, Laura M. Chopra, Sunandini Choi, Sungyoung Wu, Jun Langer, Robert Karnik, Rohit Farokhzad, Omid C. |
author_sort | Lim, Jong-Min |
collection | PubMed |
description | [Image: see text] High-throughput production of nanoparticles (NPs) with controlled quality is critical for their clinical translation into effective nanomedicines for diagnostics and therapeutics. Here we report a simple and versatile coaxial turbulent jet mixer that can synthesize a variety of NPs at high throughput up to 3 kg/d, while maintaining the advantages of homogeneity, reproducibility, and tunability that are normally accessible only in specialized microscale mixing devices. The device fabrication does not require specialized machining and is easy to operate. As one example, we show reproducible, high-throughput formulation of siRNA-polyelectrolyte polyplex NPs that exhibit effective gene knockdown but exhibit significant dependence on batch size when formulated using conventional methods. The coaxial turbulent jet mixer can accelerate the development of nanomedicines by providing a robust and versatile platform for preparation of NPs at throughputs suitable for in vivo studies, clinical trials, and industrial-scale production. |
format | Online Article Text |
id | pubmed-4072409 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | American Chemical Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-40724092015-05-13 Ultra-High Throughput Synthesis of Nanoparticles with Homogeneous Size Distribution Using a Coaxial Turbulent Jet Mixer Lim, Jong-Min Swami, Archana Gilson, Laura M. Chopra, Sunandini Choi, Sungyoung Wu, Jun Langer, Robert Karnik, Rohit Farokhzad, Omid C. ACS Nano [Image: see text] High-throughput production of nanoparticles (NPs) with controlled quality is critical for their clinical translation into effective nanomedicines for diagnostics and therapeutics. Here we report a simple and versatile coaxial turbulent jet mixer that can synthesize a variety of NPs at high throughput up to 3 kg/d, while maintaining the advantages of homogeneity, reproducibility, and tunability that are normally accessible only in specialized microscale mixing devices. The device fabrication does not require specialized machining and is easy to operate. As one example, we show reproducible, high-throughput formulation of siRNA-polyelectrolyte polyplex NPs that exhibit effective gene knockdown but exhibit significant dependence on batch size when formulated using conventional methods. The coaxial turbulent jet mixer can accelerate the development of nanomedicines by providing a robust and versatile platform for preparation of NPs at throughputs suitable for in vivo studies, clinical trials, and industrial-scale production. American Chemical Society 2014-05-13 2014-06-24 /pmc/articles/PMC4072409/ /pubmed/24824296 http://dx.doi.org/10.1021/nn501371n Text en Copyright © 2014 American Chemical Society Open Access on 05/13/2015 |
spellingShingle | Lim, Jong-Min Swami, Archana Gilson, Laura M. Chopra, Sunandini Choi, Sungyoung Wu, Jun Langer, Robert Karnik, Rohit Farokhzad, Omid C. Ultra-High Throughput Synthesis of Nanoparticles with Homogeneous Size Distribution Using a Coaxial Turbulent Jet Mixer |
title | Ultra-High Throughput Synthesis of Nanoparticles with Homogeneous Size Distribution Using a Coaxial Turbulent Jet Mixer |
title_full | Ultra-High Throughput Synthesis of Nanoparticles with Homogeneous Size Distribution Using a Coaxial Turbulent Jet Mixer |
title_fullStr | Ultra-High Throughput Synthesis of Nanoparticles with Homogeneous Size Distribution Using a Coaxial Turbulent Jet Mixer |
title_full_unstemmed | Ultra-High Throughput Synthesis of Nanoparticles with Homogeneous Size Distribution Using a Coaxial Turbulent Jet Mixer |
title_short | Ultra-High Throughput Synthesis of Nanoparticles with Homogeneous Size Distribution Using a Coaxial Turbulent Jet Mixer |
title_sort | ultra-high throughput synthesis of nanoparticles with homogeneous size distribution using a coaxial turbulent jet mixer |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4072409/ https://www.ncbi.nlm.nih.gov/pubmed/24824296 http://dx.doi.org/10.1021/nn501371n |
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