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Influenza Vaccination Accelerates Recovery of Ferrets from Lymphopenia
Ferrets are a useful animal model for human influenza virus infections, since they closely mimic the pathogenesis of influenza viruses observed in humans. However, a lack of reagents, especially for flow cytometry of immune cell subsets, has limited research in this model. Here we use a panel of pri...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4072694/ https://www.ncbi.nlm.nih.gov/pubmed/24968319 http://dx.doi.org/10.1371/journal.pone.0100926 |
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author | Music, Nedzad Reber, Adrian J. Lipatov, Aleksandr S. Kamal, Ram P. Blanchfield, Kristy Wilson, Jason R. Donis, Ruben O. Katz, Jacqueline M. York, Ian A. |
author_facet | Music, Nedzad Reber, Adrian J. Lipatov, Aleksandr S. Kamal, Ram P. Blanchfield, Kristy Wilson, Jason R. Donis, Ruben O. Katz, Jacqueline M. York, Ian A. |
author_sort | Music, Nedzad |
collection | PubMed |
description | Ferrets are a useful animal model for human influenza virus infections, since they closely mimic the pathogenesis of influenza viruses observed in humans. However, a lack of reagents, especially for flow cytometry of immune cell subsets, has limited research in this model. Here we use a panel of primarily species cross-reactive antibodies to identify ferret T cells, cytotoxic T lymphocytes (CTL), B cells, and granulocytes in peripheral blood. Following infection with seasonal H3N2 or H1N1pdm09 influenza viruses, these cell types showed rapid and dramatic changes in frequency, even though clinically the infections were mild. The loss of B cells and CD4 and CD8 T cells, and the increase in neutrophils, were especially marked 1–2 days after infection, when about 90% of CD8+ T cells disappeared from the peripheral blood. The different virus strains led to different kinetics of leukocyte subset alterations. Vaccination with homologous vaccine reduced clinical symptoms slightly, but led to a much more rapid return to normal leukocyte parameters. Assessment of clinical symptoms may underestimate the effectiveness of influenza vaccine in restoring homeostasis. |
format | Online Article Text |
id | pubmed-4072694 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-40726942014-07-02 Influenza Vaccination Accelerates Recovery of Ferrets from Lymphopenia Music, Nedzad Reber, Adrian J. Lipatov, Aleksandr S. Kamal, Ram P. Blanchfield, Kristy Wilson, Jason R. Donis, Ruben O. Katz, Jacqueline M. York, Ian A. PLoS One Research Article Ferrets are a useful animal model for human influenza virus infections, since they closely mimic the pathogenesis of influenza viruses observed in humans. However, a lack of reagents, especially for flow cytometry of immune cell subsets, has limited research in this model. Here we use a panel of primarily species cross-reactive antibodies to identify ferret T cells, cytotoxic T lymphocytes (CTL), B cells, and granulocytes in peripheral blood. Following infection with seasonal H3N2 or H1N1pdm09 influenza viruses, these cell types showed rapid and dramatic changes in frequency, even though clinically the infections were mild. The loss of B cells and CD4 and CD8 T cells, and the increase in neutrophils, were especially marked 1–2 days after infection, when about 90% of CD8+ T cells disappeared from the peripheral blood. The different virus strains led to different kinetics of leukocyte subset alterations. Vaccination with homologous vaccine reduced clinical symptoms slightly, but led to a much more rapid return to normal leukocyte parameters. Assessment of clinical symptoms may underestimate the effectiveness of influenza vaccine in restoring homeostasis. Public Library of Science 2014-06-26 /pmc/articles/PMC4072694/ /pubmed/24968319 http://dx.doi.org/10.1371/journal.pone.0100926 Text en https://creativecommons.org/publicdomain/zero/1.0/ This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. |
spellingShingle | Research Article Music, Nedzad Reber, Adrian J. Lipatov, Aleksandr S. Kamal, Ram P. Blanchfield, Kristy Wilson, Jason R. Donis, Ruben O. Katz, Jacqueline M. York, Ian A. Influenza Vaccination Accelerates Recovery of Ferrets from Lymphopenia |
title | Influenza Vaccination Accelerates Recovery of Ferrets from Lymphopenia |
title_full | Influenza Vaccination Accelerates Recovery of Ferrets from Lymphopenia |
title_fullStr | Influenza Vaccination Accelerates Recovery of Ferrets from Lymphopenia |
title_full_unstemmed | Influenza Vaccination Accelerates Recovery of Ferrets from Lymphopenia |
title_short | Influenza Vaccination Accelerates Recovery of Ferrets from Lymphopenia |
title_sort | influenza vaccination accelerates recovery of ferrets from lymphopenia |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4072694/ https://www.ncbi.nlm.nih.gov/pubmed/24968319 http://dx.doi.org/10.1371/journal.pone.0100926 |
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