Selection and Characterization of a DNA Aptamer That Can Discriminate between cJun/cJun and cJun/cFos

The AP-1 family of transcriptional activators plays pivotal roles in regulating a wide range of biological processes from the immune response to tumorigenesis. Determining the roles of specific AP-1 dimers in cells, however, has remained challenging because common molecular biology techniques are un...

Descripción completa

Detalles Bibliográficos
Autores principales: Walters, Ryan D., McSwiggen, David T., Goodrich, James A., Kugel, Jennifer F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4072727/
https://www.ncbi.nlm.nih.gov/pubmed/24968026
http://dx.doi.org/10.1371/journal.pone.0101015
Descripción
Sumario:The AP-1 family of transcriptional activators plays pivotal roles in regulating a wide range of biological processes from the immune response to tumorigenesis. Determining the roles of specific AP-1 dimers in cells, however, has remained challenging because common molecular biology techniques are unable to distinguish between the role of, for example, cJun/cJun homodimers versus cJun/cFos heterodimers. Here we used SELEX (systematic evolution of ligands by exponential enrichment) to identify and characterize DNA aptamers that are >100-fold more specific for binding cJun/cJun compared to cJun/cFos, setting the foundation to investigate the biological functions of different AP-1 dimer compositions.

Ejemplares similares