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Influence of Handling Conditions on the Establishment and Propagation of Head and Neck Cancer Patient Derived Xenografts

BACKGROUND: Patient derived xenografts (PDXs) for head and neck cancer (HNC) and other cancers represent powerful research platforms. Most groups implant patient tissue into immunodeficient mice immediately although the significance of this time interval is anecdotal. We tested the hypothesis that t...

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Autores principales: Stein, Andrew P., Saha, Sandeep, Liu, Cheng Z., Hartig, Gregory K., Lambert, Paul F., Kimple, Randall J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4072729/
https://www.ncbi.nlm.nih.gov/pubmed/24967635
http://dx.doi.org/10.1371/journal.pone.0100995
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author Stein, Andrew P.
Saha, Sandeep
Liu, Cheng Z.
Hartig, Gregory K.
Lambert, Paul F.
Kimple, Randall J.
author_facet Stein, Andrew P.
Saha, Sandeep
Liu, Cheng Z.
Hartig, Gregory K.
Lambert, Paul F.
Kimple, Randall J.
author_sort Stein, Andrew P.
collection PubMed
description BACKGROUND: Patient derived xenografts (PDXs) for head and neck cancer (HNC) and other cancers represent powerful research platforms. Most groups implant patient tissue into immunodeficient mice immediately although the significance of this time interval is anecdotal. We tested the hypothesis that the time from tumor excision to implantation is crucial for PDX passaging and establishment. METHODS: We examined whether time or storage medium affected PDX viability for passaging two established HNC PDXs (UW-SCC34, UW-SCC52). Tumors were harvested, stored in ice-cold media or saline for 0–48 hours, and implanted into new mice. Tumor growth was compared by two-way ANOVA with respect to time and storage condition. Three new HNC PDXs (UW-SCC63-65) were generated by implanting patient tissue into mice immediately (Time 0) and 24 hours after receiving tissue from the operating room. RESULTS: Similar quantities of tumor were implanted into each mouse. At the end of the experiment, no significant difference was seen in mean tumor weight between the media and saline storage conditions for UW-SCC34 or UW-SCC52 (p = 0.650 and p = 0.177, respectively). No difference in tumor formation prevalence was seen on the basis of time from harvest to implantation (≥13 of 16 tumors grew at every time point). Histological analysis showed strong similarity to the initial tumor across all groups. Tumors developed at both Time 0 and 24 hours for UW-SCC63 and UW-SCC64. CONCLUSIONS: We demonstrated that neither storage medium nor time from tumor excision to implantation (up to 48 hours) affected viability or histological differentiation in a subsequent passage for two HNC PDXs. Moreover, we revealed that fresh patient tissue is viable up to 24 hours post-resection. This information is important as it applies to the development and sharing of PDXs.
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spelling pubmed-40727292014-07-02 Influence of Handling Conditions on the Establishment and Propagation of Head and Neck Cancer Patient Derived Xenografts Stein, Andrew P. Saha, Sandeep Liu, Cheng Z. Hartig, Gregory K. Lambert, Paul F. Kimple, Randall J. PLoS One Research Article BACKGROUND: Patient derived xenografts (PDXs) for head and neck cancer (HNC) and other cancers represent powerful research platforms. Most groups implant patient tissue into immunodeficient mice immediately although the significance of this time interval is anecdotal. We tested the hypothesis that the time from tumor excision to implantation is crucial for PDX passaging and establishment. METHODS: We examined whether time or storage medium affected PDX viability for passaging two established HNC PDXs (UW-SCC34, UW-SCC52). Tumors were harvested, stored in ice-cold media or saline for 0–48 hours, and implanted into new mice. Tumor growth was compared by two-way ANOVA with respect to time and storage condition. Three new HNC PDXs (UW-SCC63-65) were generated by implanting patient tissue into mice immediately (Time 0) and 24 hours after receiving tissue from the operating room. RESULTS: Similar quantities of tumor were implanted into each mouse. At the end of the experiment, no significant difference was seen in mean tumor weight between the media and saline storage conditions for UW-SCC34 or UW-SCC52 (p = 0.650 and p = 0.177, respectively). No difference in tumor formation prevalence was seen on the basis of time from harvest to implantation (≥13 of 16 tumors grew at every time point). Histological analysis showed strong similarity to the initial tumor across all groups. Tumors developed at both Time 0 and 24 hours for UW-SCC63 and UW-SCC64. CONCLUSIONS: We demonstrated that neither storage medium nor time from tumor excision to implantation (up to 48 hours) affected viability or histological differentiation in a subsequent passage for two HNC PDXs. Moreover, we revealed that fresh patient tissue is viable up to 24 hours post-resection. This information is important as it applies to the development and sharing of PDXs. Public Library of Science 2014-06-26 /pmc/articles/PMC4072729/ /pubmed/24967635 http://dx.doi.org/10.1371/journal.pone.0100995 Text en © 2014 Stein et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Stein, Andrew P.
Saha, Sandeep
Liu, Cheng Z.
Hartig, Gregory K.
Lambert, Paul F.
Kimple, Randall J.
Influence of Handling Conditions on the Establishment and Propagation of Head and Neck Cancer Patient Derived Xenografts
title Influence of Handling Conditions on the Establishment and Propagation of Head and Neck Cancer Patient Derived Xenografts
title_full Influence of Handling Conditions on the Establishment and Propagation of Head and Neck Cancer Patient Derived Xenografts
title_fullStr Influence of Handling Conditions on the Establishment and Propagation of Head and Neck Cancer Patient Derived Xenografts
title_full_unstemmed Influence of Handling Conditions on the Establishment and Propagation of Head and Neck Cancer Patient Derived Xenografts
title_short Influence of Handling Conditions on the Establishment and Propagation of Head and Neck Cancer Patient Derived Xenografts
title_sort influence of handling conditions on the establishment and propagation of head and neck cancer patient derived xenografts
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4072729/
https://www.ncbi.nlm.nih.gov/pubmed/24967635
http://dx.doi.org/10.1371/journal.pone.0100995
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