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Assessment of Genetic Diversity among Barley Cultivars and Breeding Lines Adapted to the US Pacific Northwest, and Its Implications in Breeding Barley for Imidazolinone-Resistance

Extensive application of imidazolinone (IMI) herbicides had a significant impact on barley productivity contributing to a continuous decline in its acreage over the last two decades. A possible solution to this problem is to transfer IMI-resistance from a recently characterized mutation in the ‘Bob’...

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Autores principales: Rustgi, Sachin, Matanguihan, Janet, Mejías, Jaime H., Gemini, Richa, Brew-Appiah, Rhoda A. T., Wen, Nuan, Osorio, Claudia, Ankrah, Nii, Murphy, Kevin M., von Wettstein, Diter
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4072767/
https://www.ncbi.nlm.nih.gov/pubmed/24967712
http://dx.doi.org/10.1371/journal.pone.0100998
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author Rustgi, Sachin
Matanguihan, Janet
Mejías, Jaime H.
Gemini, Richa
Brew-Appiah, Rhoda A. T.
Wen, Nuan
Osorio, Claudia
Ankrah, Nii
Murphy, Kevin M.
von Wettstein, Diter
author_facet Rustgi, Sachin
Matanguihan, Janet
Mejías, Jaime H.
Gemini, Richa
Brew-Appiah, Rhoda A. T.
Wen, Nuan
Osorio, Claudia
Ankrah, Nii
Murphy, Kevin M.
von Wettstein, Diter
author_sort Rustgi, Sachin
collection PubMed
description Extensive application of imidazolinone (IMI) herbicides had a significant impact on barley productivity contributing to a continuous decline in its acreage over the last two decades. A possible solution to this problem is to transfer IMI-resistance from a recently characterized mutation in the ‘Bob’ barley AHAS (acetohydroxy acid synthase) gene to other food, feed and malting barley cultivars. We focused our efforts on transferring IMI-resistance to barley varieties adapted to the US Pacific Northwest (PNW), since it comprises ∼23% (335,000 ha) of the US agricultural land under barley production. To effectively breed for IMI-resistance, we studied the genetic diversity among 13 two-rowed spring barley cultivars/breeding-lines from the PNW using 61 microsatellite markers, and selected six barley genotypes that showed medium to high genetic dissimilarity with the ‘Bob’ AHAS mutant. The six selected genotypes were used to make 29–53 crosses with the AHAS mutant and a range of 358–471 F(1) seeds were obtained. To make informed selection for the recovery of the recipient parent genome, the genetic location of the AHAS gene was determined and its genetic nature assessed. Large F(2) populations ranging in size from 2158–2846 individuals were evaluated for herbicide resistance and seedling vigor. Based on the results, F(3) lines from the six most vigorous F(2) genotypes per cross combination were evaluated for their genetic background. A range of 20%–90% recovery of the recipient parent genome for the carrier chromosome was observed. An effort was made to determine the critical dose of herbicide to distinguish between heterozygotes and homozygotes for the mutant allele. Results suggested that the mutant can survive up to the 10× field recommended dose of herbicide, and the 8× and 10× herbicide doses can distinguish between the two AHAS mutant genotypes. Finally, implications of this research in sustaining barley productivity in the PNW are discussed.
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spelling pubmed-40727672014-07-02 Assessment of Genetic Diversity among Barley Cultivars and Breeding Lines Adapted to the US Pacific Northwest, and Its Implications in Breeding Barley for Imidazolinone-Resistance Rustgi, Sachin Matanguihan, Janet Mejías, Jaime H. Gemini, Richa Brew-Appiah, Rhoda A. T. Wen, Nuan Osorio, Claudia Ankrah, Nii Murphy, Kevin M. von Wettstein, Diter PLoS One Research Article Extensive application of imidazolinone (IMI) herbicides had a significant impact on barley productivity contributing to a continuous decline in its acreage over the last two decades. A possible solution to this problem is to transfer IMI-resistance from a recently characterized mutation in the ‘Bob’ barley AHAS (acetohydroxy acid synthase) gene to other food, feed and malting barley cultivars. We focused our efforts on transferring IMI-resistance to barley varieties adapted to the US Pacific Northwest (PNW), since it comprises ∼23% (335,000 ha) of the US agricultural land under barley production. To effectively breed for IMI-resistance, we studied the genetic diversity among 13 two-rowed spring barley cultivars/breeding-lines from the PNW using 61 microsatellite markers, and selected six barley genotypes that showed medium to high genetic dissimilarity with the ‘Bob’ AHAS mutant. The six selected genotypes were used to make 29–53 crosses with the AHAS mutant and a range of 358–471 F(1) seeds were obtained. To make informed selection for the recovery of the recipient parent genome, the genetic location of the AHAS gene was determined and its genetic nature assessed. Large F(2) populations ranging in size from 2158–2846 individuals were evaluated for herbicide resistance and seedling vigor. Based on the results, F(3) lines from the six most vigorous F(2) genotypes per cross combination were evaluated for their genetic background. A range of 20%–90% recovery of the recipient parent genome for the carrier chromosome was observed. An effort was made to determine the critical dose of herbicide to distinguish between heterozygotes and homozygotes for the mutant allele. Results suggested that the mutant can survive up to the 10× field recommended dose of herbicide, and the 8× and 10× herbicide doses can distinguish between the two AHAS mutant genotypes. Finally, implications of this research in sustaining barley productivity in the PNW are discussed. Public Library of Science 2014-06-26 /pmc/articles/PMC4072767/ /pubmed/24967712 http://dx.doi.org/10.1371/journal.pone.0100998 Text en © 2014 Rustgi et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Rustgi, Sachin
Matanguihan, Janet
Mejías, Jaime H.
Gemini, Richa
Brew-Appiah, Rhoda A. T.
Wen, Nuan
Osorio, Claudia
Ankrah, Nii
Murphy, Kevin M.
von Wettstein, Diter
Assessment of Genetic Diversity among Barley Cultivars and Breeding Lines Adapted to the US Pacific Northwest, and Its Implications in Breeding Barley for Imidazolinone-Resistance
title Assessment of Genetic Diversity among Barley Cultivars and Breeding Lines Adapted to the US Pacific Northwest, and Its Implications in Breeding Barley for Imidazolinone-Resistance
title_full Assessment of Genetic Diversity among Barley Cultivars and Breeding Lines Adapted to the US Pacific Northwest, and Its Implications in Breeding Barley for Imidazolinone-Resistance
title_fullStr Assessment of Genetic Diversity among Barley Cultivars and Breeding Lines Adapted to the US Pacific Northwest, and Its Implications in Breeding Barley for Imidazolinone-Resistance
title_full_unstemmed Assessment of Genetic Diversity among Barley Cultivars and Breeding Lines Adapted to the US Pacific Northwest, and Its Implications in Breeding Barley for Imidazolinone-Resistance
title_short Assessment of Genetic Diversity among Barley Cultivars and Breeding Lines Adapted to the US Pacific Northwest, and Its Implications in Breeding Barley for Imidazolinone-Resistance
title_sort assessment of genetic diversity among barley cultivars and breeding lines adapted to the us pacific northwest, and its implications in breeding barley for imidazolinone-resistance
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4072767/
https://www.ncbi.nlm.nih.gov/pubmed/24967712
http://dx.doi.org/10.1371/journal.pone.0100998
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