HopW1 from Pseudomonas syringae Disrupts the Actin Cytoskeleton to Promote Virulence in Arabidopsis
A central mechanism of virulence of extracellular bacterial pathogens is the injection into host cells of effector proteins that modify host cellular functions. HopW1 is an effector injected by the type III secretion system that increases the growth of the plant pathogen Pseudomonas syringae on the...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2014
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4072799/ https://www.ncbi.nlm.nih.gov/pubmed/24968323 http://dx.doi.org/10.1371/journal.ppat.1004232 |
_version_ | 1782323025289412608 |
---|---|
author | Kang, Yongsung Jelenska, Joanna Cecchini, Nicolas M. Li, Yujie Lee, Min Woo Kovar, David R. Greenberg, Jean T. |
author_facet | Kang, Yongsung Jelenska, Joanna Cecchini, Nicolas M. Li, Yujie Lee, Min Woo Kovar, David R. Greenberg, Jean T. |
author_sort | Kang, Yongsung |
collection | PubMed |
description | A central mechanism of virulence of extracellular bacterial pathogens is the injection into host cells of effector proteins that modify host cellular functions. HopW1 is an effector injected by the type III secretion system that increases the growth of the plant pathogen Pseudomonas syringae on the Columbia accession of Arabidopsis. When delivered by P. syringae into plant cells, HopW1 causes a reduction in the filamentous actin (F-actin) network and the inhibition of endocytosis, a known actin-dependent process. When directly produced in plants, HopW1 forms complexes with actin, disrupts the actin cytoskeleton and inhibits endocytosis as well as the trafficking of certain proteins to vacuoles. The C-terminal region of HopW1 can reduce the length of actin filaments and therefore solubilize F-actin in vitro. Thus, HopW1 acts by disrupting the actin cytoskeleton and the cell biological processes that depend on actin, which in turn are needed for restricting P. syringae growth in Arabidopsis. |
format | Online Article Text |
id | pubmed-4072799 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-40727992014-07-02 HopW1 from Pseudomonas syringae Disrupts the Actin Cytoskeleton to Promote Virulence in Arabidopsis Kang, Yongsung Jelenska, Joanna Cecchini, Nicolas M. Li, Yujie Lee, Min Woo Kovar, David R. Greenberg, Jean T. PLoS Pathog Research Article A central mechanism of virulence of extracellular bacterial pathogens is the injection into host cells of effector proteins that modify host cellular functions. HopW1 is an effector injected by the type III secretion system that increases the growth of the plant pathogen Pseudomonas syringae on the Columbia accession of Arabidopsis. When delivered by P. syringae into plant cells, HopW1 causes a reduction in the filamentous actin (F-actin) network and the inhibition of endocytosis, a known actin-dependent process. When directly produced in plants, HopW1 forms complexes with actin, disrupts the actin cytoskeleton and inhibits endocytosis as well as the trafficking of certain proteins to vacuoles. The C-terminal region of HopW1 can reduce the length of actin filaments and therefore solubilize F-actin in vitro. Thus, HopW1 acts by disrupting the actin cytoskeleton and the cell biological processes that depend on actin, which in turn are needed for restricting P. syringae growth in Arabidopsis. Public Library of Science 2014-06-26 /pmc/articles/PMC4072799/ /pubmed/24968323 http://dx.doi.org/10.1371/journal.ppat.1004232 Text en © 2014 Kang et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Kang, Yongsung Jelenska, Joanna Cecchini, Nicolas M. Li, Yujie Lee, Min Woo Kovar, David R. Greenberg, Jean T. HopW1 from Pseudomonas syringae Disrupts the Actin Cytoskeleton to Promote Virulence in Arabidopsis |
title | HopW1 from Pseudomonas syringae Disrupts the Actin Cytoskeleton to Promote Virulence in Arabidopsis |
title_full | HopW1 from Pseudomonas syringae Disrupts the Actin Cytoskeleton to Promote Virulence in Arabidopsis |
title_fullStr | HopW1 from Pseudomonas syringae Disrupts the Actin Cytoskeleton to Promote Virulence in Arabidopsis |
title_full_unstemmed | HopW1 from Pseudomonas syringae Disrupts the Actin Cytoskeleton to Promote Virulence in Arabidopsis |
title_short | HopW1 from Pseudomonas syringae Disrupts the Actin Cytoskeleton to Promote Virulence in Arabidopsis |
title_sort | hopw1 from pseudomonas syringae disrupts the actin cytoskeleton to promote virulence in arabidopsis |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4072799/ https://www.ncbi.nlm.nih.gov/pubmed/24968323 http://dx.doi.org/10.1371/journal.ppat.1004232 |
work_keys_str_mv | AT kangyongsung hopw1frompseudomonassyringaedisruptstheactincytoskeletontopromotevirulenceinarabidopsis AT jelenskajoanna hopw1frompseudomonassyringaedisruptstheactincytoskeletontopromotevirulenceinarabidopsis AT cecchininicolasm hopw1frompseudomonassyringaedisruptstheactincytoskeletontopromotevirulenceinarabidopsis AT liyujie hopw1frompseudomonassyringaedisruptstheactincytoskeletontopromotevirulenceinarabidopsis AT leeminwoo hopw1frompseudomonassyringaedisruptstheactincytoskeletontopromotevirulenceinarabidopsis AT kovardavidr hopw1frompseudomonassyringaedisruptstheactincytoskeletontopromotevirulenceinarabidopsis AT greenbergjeant hopw1frompseudomonassyringaedisruptstheactincytoskeletontopromotevirulenceinarabidopsis |