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Ganoderma formosanum polysaccharides attenuate Th2 inflammation and airway hyperresponsiveness in a murine model of allergic asthma

Allergic asthma is an inflammatory disease of the airways mediated by Th2 immune responses and characterized by airway hyperresponsiveness (AHR). Fungi of the genus Ganoderma are basidiomycetes that have been used in traditional Asian medicine for centuries. We recently found that PS-F2, a polysacch...

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Autores principales: Pi, Chia-Chen, Wang, Hui-Yi, Lu, Chiu-Ying, Lu, Frank Leigh, Chen, Chun-Jen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer International Publishing 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4072879/
https://www.ncbi.nlm.nih.gov/pubmed/25019045
http://dx.doi.org/10.1186/2193-1801-3-297
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author Pi, Chia-Chen
Wang, Hui-Yi
Lu, Chiu-Ying
Lu, Frank Leigh
Chen, Chun-Jen
author_facet Pi, Chia-Chen
Wang, Hui-Yi
Lu, Chiu-Ying
Lu, Frank Leigh
Chen, Chun-Jen
author_sort Pi, Chia-Chen
collection PubMed
description Allergic asthma is an inflammatory disease of the airways mediated by Th2 immune responses and characterized by airway hyperresponsiveness (AHR). Fungi of the genus Ganoderma are basidiomycetes that have been used in traditional Asian medicine for centuries. We recently found that PS-F2, a polysaccharide fraction purified from the submerged culture broth of Ganoderma formosanum, stimulates the activation of dendritic cells and primes a T helper 1 (Th1)-polarized adaptive immune response. This study was designed to investigate whether the Th1 adjuvant properties of PS-F2 could suppress the development of allergic asthma in a mouse model. BALB/c mice were sensitized by repeated immunization with chicken ovalbumin (OVA) and alum, followed by intranasal challenge of OVA to induce acute asthma. PS-F2 administration during the course of OVA sensitization and challenge effectively prevented AHR development, OVA-specific IgE and IgG1 production, bronchial inflammation, and Th2 cytokine production. Our data indicate that PS-F2 has a potential to be used for the prevention of allergic asthma. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi: 10.1186/2193-1801-3-297) contains supplementary material, which is available to authorized users.
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spelling pubmed-40728792014-07-11 Ganoderma formosanum polysaccharides attenuate Th2 inflammation and airway hyperresponsiveness in a murine model of allergic asthma Pi, Chia-Chen Wang, Hui-Yi Lu, Chiu-Ying Lu, Frank Leigh Chen, Chun-Jen Springerplus Research Allergic asthma is an inflammatory disease of the airways mediated by Th2 immune responses and characterized by airway hyperresponsiveness (AHR). Fungi of the genus Ganoderma are basidiomycetes that have been used in traditional Asian medicine for centuries. We recently found that PS-F2, a polysaccharide fraction purified from the submerged culture broth of Ganoderma formosanum, stimulates the activation of dendritic cells and primes a T helper 1 (Th1)-polarized adaptive immune response. This study was designed to investigate whether the Th1 adjuvant properties of PS-F2 could suppress the development of allergic asthma in a mouse model. BALB/c mice were sensitized by repeated immunization with chicken ovalbumin (OVA) and alum, followed by intranasal challenge of OVA to induce acute asthma. PS-F2 administration during the course of OVA sensitization and challenge effectively prevented AHR development, OVA-specific IgE and IgG1 production, bronchial inflammation, and Th2 cytokine production. Our data indicate that PS-F2 has a potential to be used for the prevention of allergic asthma. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi: 10.1186/2193-1801-3-297) contains supplementary material, which is available to authorized users. Springer International Publishing 2014-06-12 /pmc/articles/PMC4072879/ /pubmed/25019045 http://dx.doi.org/10.1186/2193-1801-3-297 Text en © Pi et al.; licensee Springer. 2014 This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited.
spellingShingle Research
Pi, Chia-Chen
Wang, Hui-Yi
Lu, Chiu-Ying
Lu, Frank Leigh
Chen, Chun-Jen
Ganoderma formosanum polysaccharides attenuate Th2 inflammation and airway hyperresponsiveness in a murine model of allergic asthma
title Ganoderma formosanum polysaccharides attenuate Th2 inflammation and airway hyperresponsiveness in a murine model of allergic asthma
title_full Ganoderma formosanum polysaccharides attenuate Th2 inflammation and airway hyperresponsiveness in a murine model of allergic asthma
title_fullStr Ganoderma formosanum polysaccharides attenuate Th2 inflammation and airway hyperresponsiveness in a murine model of allergic asthma
title_full_unstemmed Ganoderma formosanum polysaccharides attenuate Th2 inflammation and airway hyperresponsiveness in a murine model of allergic asthma
title_short Ganoderma formosanum polysaccharides attenuate Th2 inflammation and airway hyperresponsiveness in a murine model of allergic asthma
title_sort ganoderma formosanum polysaccharides attenuate th2 inflammation and airway hyperresponsiveness in a murine model of allergic asthma
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4072879/
https://www.ncbi.nlm.nih.gov/pubmed/25019045
http://dx.doi.org/10.1186/2193-1801-3-297
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