4sUDRB-seq: measuring genomewide transcriptional elongation rates and initiation frequencies within cells

Although transcriptional elongation by RNA polymerase II is coupled with many RNA-related processes, genomewide elongation rates remain unknown. We describe a method, called 4sUDRB-seq, based on reversible inhibition of transcription elongation coupled with tagging newly transcribed RNA with 4-thiou...

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Detalles Bibliográficos
Autores principales: Fuchs, Gilad, Voichek, Yoav, Benjamin, Sima, Gilad, Shlomit, Amit, Ido, Oren, Moshe
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2014
Materias:
Method
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4072947/
https://www.ncbi.nlm.nih.gov/pubmed/24887486
http://dx.doi.org/10.1186/gb-2014-15-5-r69

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