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Zebrafish yolk lipid processing: a tractable tool for the study of vertebrate lipid transport and metabolism

Dyslipidemias are a major cause of morbidity and mortality in the world, particularly in developed nations. Investigating lipid and lipoprotein metabolism in experimentally tractable animal models is a crucial step towards understanding and treating human dyslipidemias. The zebrafish, a well-establi...

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Autores principales: Miyares, Rosa L., de Rezende, Vitor B., Farber, Steven A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Company of Biologists Limited 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4073280/
https://www.ncbi.nlm.nih.gov/pubmed/24812437
http://dx.doi.org/10.1242/dmm.015800
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author Miyares, Rosa L.
de Rezende, Vitor B.
Farber, Steven A.
author_facet Miyares, Rosa L.
de Rezende, Vitor B.
Farber, Steven A.
author_sort Miyares, Rosa L.
collection PubMed
description Dyslipidemias are a major cause of morbidity and mortality in the world, particularly in developed nations. Investigating lipid and lipoprotein metabolism in experimentally tractable animal models is a crucial step towards understanding and treating human dyslipidemias. The zebrafish, a well-established embryological model, is emerging as a notable system for studies of lipid metabolism. Here, we describe the value of the lecithotrophic, or yolk-metabolizing, stages of the zebrafish as a model for studying lipid metabolism and lipoprotein transport. We demonstrate methods to assay yolk lipid metabolism in embryonic and larval zebrafish. Injection of labeled fatty acids into the zebrafish yolk promotes efficient uptake into the circulation and rapid metabolism. Using a genetic model for abetalipoproteinemia, we show that the uptake of labeled fatty acids into the circulation is dependent on lipoprotein production. Furthermore, we examine the metabolic fate of exogenously delivered fatty acids by assaying their incorporation into complex lipids. Moreover, we demonstrate that this technique is amenable to genetic and pharmacologic studies.
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spelling pubmed-40732802014-07-17 Zebrafish yolk lipid processing: a tractable tool for the study of vertebrate lipid transport and metabolism Miyares, Rosa L. de Rezende, Vitor B. Farber, Steven A. Dis Model Mech Resource Article Dyslipidemias are a major cause of morbidity and mortality in the world, particularly in developed nations. Investigating lipid and lipoprotein metabolism in experimentally tractable animal models is a crucial step towards understanding and treating human dyslipidemias. The zebrafish, a well-established embryological model, is emerging as a notable system for studies of lipid metabolism. Here, we describe the value of the lecithotrophic, or yolk-metabolizing, stages of the zebrafish as a model for studying lipid metabolism and lipoprotein transport. We demonstrate methods to assay yolk lipid metabolism in embryonic and larval zebrafish. Injection of labeled fatty acids into the zebrafish yolk promotes efficient uptake into the circulation and rapid metabolism. Using a genetic model for abetalipoproteinemia, we show that the uptake of labeled fatty acids into the circulation is dependent on lipoprotein production. Furthermore, we examine the metabolic fate of exogenously delivered fatty acids by assaying their incorporation into complex lipids. Moreover, we demonstrate that this technique is amenable to genetic and pharmacologic studies. The Company of Biologists Limited 2014-07 2014-05-08 /pmc/articles/PMC4073280/ /pubmed/24812437 http://dx.doi.org/10.1242/dmm.015800 Text en © 2014. Published by The Company of Biologists Ltd This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.
spellingShingle Resource Article
Miyares, Rosa L.
de Rezende, Vitor B.
Farber, Steven A.
Zebrafish yolk lipid processing: a tractable tool for the study of vertebrate lipid transport and metabolism
title Zebrafish yolk lipid processing: a tractable tool for the study of vertebrate lipid transport and metabolism
title_full Zebrafish yolk lipid processing: a tractable tool for the study of vertebrate lipid transport and metabolism
title_fullStr Zebrafish yolk lipid processing: a tractable tool for the study of vertebrate lipid transport and metabolism
title_full_unstemmed Zebrafish yolk lipid processing: a tractable tool for the study of vertebrate lipid transport and metabolism
title_short Zebrafish yolk lipid processing: a tractable tool for the study of vertebrate lipid transport and metabolism
title_sort zebrafish yolk lipid processing: a tractable tool for the study of vertebrate lipid transport and metabolism
topic Resource Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4073280/
https://www.ncbi.nlm.nih.gov/pubmed/24812437
http://dx.doi.org/10.1242/dmm.015800
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