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An Optimized Protocol for Isolating Primary Epithelial Cell Chromatin for ChIP
A critical part of generating robust chromatin immunoprecipitation (ChIP) data is the optimization of chromatin purification and size selection. This is particularly important when ChIP is combined with next-generation sequencing (ChIP-seq) to identify targets of DNA-binding proteins, genome-wide. C...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Public Library of Science
2014
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4074041/ https://www.ncbi.nlm.nih.gov/pubmed/24971909 http://dx.doi.org/10.1371/journal.pone.0100099 |