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Hemodynamic Effects of Combined Focal Cerebral Ischemia and Amyloid Protein Toxicity in a Rat Model: A Functional CT Study
BACKGROUND/OBJECTIVE: Clinical evidence indicates that cerebral ischemia (CI) and a pathological factor of Alzheimer's disease, the β-amyloid (Aβ) protein, can increase the rate of cognitive impairment in the ageing population. Using the CT Perfusion (CTP) functional imaging, we sought to inves...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4074060/ https://www.ncbi.nlm.nih.gov/pubmed/24971942 http://dx.doi.org/10.1371/journal.pone.0100575 |
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author | Yang, Jun d'Esterre, Christopher D. Amtul, Zareen Cechetto, David F. Lee, Ting Yim |
author_facet | Yang, Jun d'Esterre, Christopher D. Amtul, Zareen Cechetto, David F. Lee, Ting Yim |
author_sort | Yang, Jun |
collection | PubMed |
description | BACKGROUND/OBJECTIVE: Clinical evidence indicates that cerebral ischemia (CI) and a pathological factor of Alzheimer's disease, the β-amyloid (Aβ) protein, can increase the rate of cognitive impairment in the ageing population. Using the CT Perfusion (CTP) functional imaging, we sought to investigate the interaction between CI and the Aβ protein on cerebral hemodynamics. METHODS: A previously established rat model of CI and Aβ was used for the CTP study. Iodinated contrast was given intravenously, while serial CT images of sixteen axial slices were acquired. Cerebral blood flow (CBF) and blood volume (CBV) parametric maps were co-registered to a rat brain atlas and regions of interest were drawn on the maps. Microvascular alteration was investigated with histopathology. RESULTS: CTP results revealed that ipsilateral striatum of Aβ+CI and CI groups showed significantly lower CBF and CBV than control at the acute phase. Striatal CBF and CBV increased significantly at week 1 in the CI and Aβ+CI groups, but not in the Aβ alone or control group. Histopathology showed that average density of dilated microvessels in the ipsilateral striatum in CI and Aβ+CI groups was significantly higher than control at week 1, indicating this could be associated with hyperperfusion and hypervolemia observed from CTP results. CONCLUSION: These results demonstrate that CTP can quantitatively measure the hemodynamic disturbance on CBF and CBV functional maps in a rat model of CI interacting with Aβ. |
format | Online Article Text |
id | pubmed-4074060 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-40740602014-07-02 Hemodynamic Effects of Combined Focal Cerebral Ischemia and Amyloid Protein Toxicity in a Rat Model: A Functional CT Study Yang, Jun d'Esterre, Christopher D. Amtul, Zareen Cechetto, David F. Lee, Ting Yim PLoS One Research Article BACKGROUND/OBJECTIVE: Clinical evidence indicates that cerebral ischemia (CI) and a pathological factor of Alzheimer's disease, the β-amyloid (Aβ) protein, can increase the rate of cognitive impairment in the ageing population. Using the CT Perfusion (CTP) functional imaging, we sought to investigate the interaction between CI and the Aβ protein on cerebral hemodynamics. METHODS: A previously established rat model of CI and Aβ was used for the CTP study. Iodinated contrast was given intravenously, while serial CT images of sixteen axial slices were acquired. Cerebral blood flow (CBF) and blood volume (CBV) parametric maps were co-registered to a rat brain atlas and regions of interest were drawn on the maps. Microvascular alteration was investigated with histopathology. RESULTS: CTP results revealed that ipsilateral striatum of Aβ+CI and CI groups showed significantly lower CBF and CBV than control at the acute phase. Striatal CBF and CBV increased significantly at week 1 in the CI and Aβ+CI groups, but not in the Aβ alone or control group. Histopathology showed that average density of dilated microvessels in the ipsilateral striatum in CI and Aβ+CI groups was significantly higher than control at week 1, indicating this could be associated with hyperperfusion and hypervolemia observed from CTP results. CONCLUSION: These results demonstrate that CTP can quantitatively measure the hemodynamic disturbance on CBF and CBV functional maps in a rat model of CI interacting with Aβ. Public Library of Science 2014-06-27 /pmc/articles/PMC4074060/ /pubmed/24971942 http://dx.doi.org/10.1371/journal.pone.0100575 Text en © 2014 Yang et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Yang, Jun d'Esterre, Christopher D. Amtul, Zareen Cechetto, David F. Lee, Ting Yim Hemodynamic Effects of Combined Focal Cerebral Ischemia and Amyloid Protein Toxicity in a Rat Model: A Functional CT Study |
title | Hemodynamic Effects of Combined Focal Cerebral Ischemia and Amyloid Protein Toxicity in a Rat Model: A Functional CT Study |
title_full | Hemodynamic Effects of Combined Focal Cerebral Ischemia and Amyloid Protein Toxicity in a Rat Model: A Functional CT Study |
title_fullStr | Hemodynamic Effects of Combined Focal Cerebral Ischemia and Amyloid Protein Toxicity in a Rat Model: A Functional CT Study |
title_full_unstemmed | Hemodynamic Effects of Combined Focal Cerebral Ischemia and Amyloid Protein Toxicity in a Rat Model: A Functional CT Study |
title_short | Hemodynamic Effects of Combined Focal Cerebral Ischemia and Amyloid Protein Toxicity in a Rat Model: A Functional CT Study |
title_sort | hemodynamic effects of combined focal cerebral ischemia and amyloid protein toxicity in a rat model: a functional ct study |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4074060/ https://www.ncbi.nlm.nih.gov/pubmed/24971942 http://dx.doi.org/10.1371/journal.pone.0100575 |
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