Cargando…

Hemodynamic Effects of Combined Focal Cerebral Ischemia and Amyloid Protein Toxicity in a Rat Model: A Functional CT Study

BACKGROUND/OBJECTIVE: Clinical evidence indicates that cerebral ischemia (CI) and a pathological factor of Alzheimer's disease, the β-amyloid (Aβ) protein, can increase the rate of cognitive impairment in the ageing population. Using the CT Perfusion (CTP) functional imaging, we sought to inves...

Descripción completa

Detalles Bibliográficos
Autores principales: Yang, Jun, d'Esterre, Christopher D., Amtul, Zareen, Cechetto, David F., Lee, Ting Yim
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4074060/
https://www.ncbi.nlm.nih.gov/pubmed/24971942
http://dx.doi.org/10.1371/journal.pone.0100575
_version_ 1782323171159965696
author Yang, Jun
d'Esterre, Christopher D.
Amtul, Zareen
Cechetto, David F.
Lee, Ting Yim
author_facet Yang, Jun
d'Esterre, Christopher D.
Amtul, Zareen
Cechetto, David F.
Lee, Ting Yim
author_sort Yang, Jun
collection PubMed
description BACKGROUND/OBJECTIVE: Clinical evidence indicates that cerebral ischemia (CI) and a pathological factor of Alzheimer's disease, the β-amyloid (Aβ) protein, can increase the rate of cognitive impairment in the ageing population. Using the CT Perfusion (CTP) functional imaging, we sought to investigate the interaction between CI and the Aβ protein on cerebral hemodynamics. METHODS: A previously established rat model of CI and Aβ was used for the CTP study. Iodinated contrast was given intravenously, while serial CT images of sixteen axial slices were acquired. Cerebral blood flow (CBF) and blood volume (CBV) parametric maps were co-registered to a rat brain atlas and regions of interest were drawn on the maps. Microvascular alteration was investigated with histopathology. RESULTS: CTP results revealed that ipsilateral striatum of Aβ+CI and CI groups showed significantly lower CBF and CBV than control at the acute phase. Striatal CBF and CBV increased significantly at week 1 in the CI and Aβ+CI groups, but not in the Aβ alone or control group. Histopathology showed that average density of dilated microvessels in the ipsilateral striatum in CI and Aβ+CI groups was significantly higher than control at week 1, indicating this could be associated with hyperperfusion and hypervolemia observed from CTP results. CONCLUSION: These results demonstrate that CTP can quantitatively measure the hemodynamic disturbance on CBF and CBV functional maps in a rat model of CI interacting with Aβ.
format Online
Article
Text
id pubmed-4074060
institution National Center for Biotechnology Information
language English
publishDate 2014
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-40740602014-07-02 Hemodynamic Effects of Combined Focal Cerebral Ischemia and Amyloid Protein Toxicity in a Rat Model: A Functional CT Study Yang, Jun d'Esterre, Christopher D. Amtul, Zareen Cechetto, David F. Lee, Ting Yim PLoS One Research Article BACKGROUND/OBJECTIVE: Clinical evidence indicates that cerebral ischemia (CI) and a pathological factor of Alzheimer's disease, the β-amyloid (Aβ) protein, can increase the rate of cognitive impairment in the ageing population. Using the CT Perfusion (CTP) functional imaging, we sought to investigate the interaction between CI and the Aβ protein on cerebral hemodynamics. METHODS: A previously established rat model of CI and Aβ was used for the CTP study. Iodinated contrast was given intravenously, while serial CT images of sixteen axial slices were acquired. Cerebral blood flow (CBF) and blood volume (CBV) parametric maps were co-registered to a rat brain atlas and regions of interest were drawn on the maps. Microvascular alteration was investigated with histopathology. RESULTS: CTP results revealed that ipsilateral striatum of Aβ+CI and CI groups showed significantly lower CBF and CBV than control at the acute phase. Striatal CBF and CBV increased significantly at week 1 in the CI and Aβ+CI groups, but not in the Aβ alone or control group. Histopathology showed that average density of dilated microvessels in the ipsilateral striatum in CI and Aβ+CI groups was significantly higher than control at week 1, indicating this could be associated with hyperperfusion and hypervolemia observed from CTP results. CONCLUSION: These results demonstrate that CTP can quantitatively measure the hemodynamic disturbance on CBF and CBV functional maps in a rat model of CI interacting with Aβ. Public Library of Science 2014-06-27 /pmc/articles/PMC4074060/ /pubmed/24971942 http://dx.doi.org/10.1371/journal.pone.0100575 Text en © 2014 Yang et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Yang, Jun
d'Esterre, Christopher D.
Amtul, Zareen
Cechetto, David F.
Lee, Ting Yim
Hemodynamic Effects of Combined Focal Cerebral Ischemia and Amyloid Protein Toxicity in a Rat Model: A Functional CT Study
title Hemodynamic Effects of Combined Focal Cerebral Ischemia and Amyloid Protein Toxicity in a Rat Model: A Functional CT Study
title_full Hemodynamic Effects of Combined Focal Cerebral Ischemia and Amyloid Protein Toxicity in a Rat Model: A Functional CT Study
title_fullStr Hemodynamic Effects of Combined Focal Cerebral Ischemia and Amyloid Protein Toxicity in a Rat Model: A Functional CT Study
title_full_unstemmed Hemodynamic Effects of Combined Focal Cerebral Ischemia and Amyloid Protein Toxicity in a Rat Model: A Functional CT Study
title_short Hemodynamic Effects of Combined Focal Cerebral Ischemia and Amyloid Protein Toxicity in a Rat Model: A Functional CT Study
title_sort hemodynamic effects of combined focal cerebral ischemia and amyloid protein toxicity in a rat model: a functional ct study
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4074060/
https://www.ncbi.nlm.nih.gov/pubmed/24971942
http://dx.doi.org/10.1371/journal.pone.0100575
work_keys_str_mv AT yangjun hemodynamiceffectsofcombinedfocalcerebralischemiaandamyloidproteintoxicityinaratmodelafunctionalctstudy
AT desterrechristopherd hemodynamiceffectsofcombinedfocalcerebralischemiaandamyloidproteintoxicityinaratmodelafunctionalctstudy
AT amtulzareen hemodynamiceffectsofcombinedfocalcerebralischemiaandamyloidproteintoxicityinaratmodelafunctionalctstudy
AT cechettodavidf hemodynamiceffectsofcombinedfocalcerebralischemiaandamyloidproteintoxicityinaratmodelafunctionalctstudy
AT leetingyim hemodynamiceffectsofcombinedfocalcerebralischemiaandamyloidproteintoxicityinaratmodelafunctionalctstudy