Cargando…

Rate of positive urine culture and double–J catheters colonization on the basis of microorganism DNA analysis

INTRODUCTION: The aim of the trial was to estimate the relationship between colonization of the Double–J catheter, and the microorganisms cultured from urine. MATERIAL AND METHODS: 60 patients, who had Double–J catheters inserted, participated in the study. All the subjects had their midstream urine...

Descripción completa

Detalles Bibliográficos
Autores principales: Kliś, Rafał, Szymkowiak, Sylwia, Madej, Adam, Blewniewski, Mariusz, Krześlak, Anna, Forma, Ewa, Bryś, Magdalena, Lipiński, Marek, Różański, Waldemar
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Polish Urological Association 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4074722/
https://www.ncbi.nlm.nih.gov/pubmed/24982789
http://dx.doi.org/10.5173/ceju.2014.01.art18
_version_ 1782323238766903296
author Kliś, Rafał
Szymkowiak, Sylwia
Madej, Adam
Blewniewski, Mariusz
Krześlak, Anna
Forma, Ewa
Bryś, Magdalena
Lipiński, Marek
Różański, Waldemar
author_facet Kliś, Rafał
Szymkowiak, Sylwia
Madej, Adam
Blewniewski, Mariusz
Krześlak, Anna
Forma, Ewa
Bryś, Magdalena
Lipiński, Marek
Różański, Waldemar
author_sort Kliś, Rafał
collection PubMed
description INTRODUCTION: The aim of the trial was to estimate the relationship between colonization of the Double–J catheter, and the microorganisms cultured from urine. MATERIAL AND METHODS: 60 patients, who had Double–J catheters inserted, participated in the study. All the subjects had their midstream urine samples taken prior to the stent insertion and removal. A negative urine culture before catheterization was mandatory to participate in the study. The patients were assigned into three subgroups, according to stenting duration: 1) 20 to 30 days (18 cases); 2) 30 to 90 days (30 cases); 3) longer than 90 days (12 cases). Bacterial and fungal DNA was identified using electrophoresis in polyacrylamide gel with a denaturing gradient (PCR–DGGE). The relationship between the genetic analysis of the catheter and the urine culture was estimated. RESULTS: Urine cultures were positive in only 8 patients, while Double–J catheter analyses were positive in all cases. In 2 cases one type of microorganism was isolated from the stent surface while the remaining 58 catheters were colonized by more than one pathogen. In three cases fungi were isolated. There were only three types of pathogens cultured from urine specimens. Urine and stent cultures were consistent in 5 cases. In 3 cases urine culture and stent analysis were not consistent. CONCLUSIONS: Double–J catheter retention in the urinary tract is associated with an extremely high risk of bacterial colonization, while the risk of urine infection is about 8–fold lower. There is a great inconsistency between urine infection and catheter colonization, indicating a low predictive value of urine culture for estimating stent colonization.
format Online
Article
Text
id pubmed-4074722
institution National Center for Biotechnology Information
language English
publishDate 2014
publisher Polish Urological Association
record_format MEDLINE/PubMed
spelling pubmed-40747222014-06-30 Rate of positive urine culture and double–J catheters colonization on the basis of microorganism DNA analysis Kliś, Rafał Szymkowiak, Sylwia Madej, Adam Blewniewski, Mariusz Krześlak, Anna Forma, Ewa Bryś, Magdalena Lipiński, Marek Różański, Waldemar Cent European J Urol Original Paper INTRODUCTION: The aim of the trial was to estimate the relationship between colonization of the Double–J catheter, and the microorganisms cultured from urine. MATERIAL AND METHODS: 60 patients, who had Double–J catheters inserted, participated in the study. All the subjects had their midstream urine samples taken prior to the stent insertion and removal. A negative urine culture before catheterization was mandatory to participate in the study. The patients were assigned into three subgroups, according to stenting duration: 1) 20 to 30 days (18 cases); 2) 30 to 90 days (30 cases); 3) longer than 90 days (12 cases). Bacterial and fungal DNA was identified using electrophoresis in polyacrylamide gel with a denaturing gradient (PCR–DGGE). The relationship between the genetic analysis of the catheter and the urine culture was estimated. RESULTS: Urine cultures were positive in only 8 patients, while Double–J catheter analyses were positive in all cases. In 2 cases one type of microorganism was isolated from the stent surface while the remaining 58 catheters were colonized by more than one pathogen. In three cases fungi were isolated. There were only three types of pathogens cultured from urine specimens. Urine and stent cultures were consistent in 5 cases. In 3 cases urine culture and stent analysis were not consistent. CONCLUSIONS: Double–J catheter retention in the urinary tract is associated with an extremely high risk of bacterial colonization, while the risk of urine infection is about 8–fold lower. There is a great inconsistency between urine infection and catheter colonization, indicating a low predictive value of urine culture for estimating stent colonization. Polish Urological Association 2014-04-17 2014 /pmc/articles/PMC4074722/ /pubmed/24982789 http://dx.doi.org/10.5173/ceju.2014.01.art18 Text en Copyright by Polish Urological Association http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution-Noncommercial 3.0 Unported License, permitting all non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Paper
Kliś, Rafał
Szymkowiak, Sylwia
Madej, Adam
Blewniewski, Mariusz
Krześlak, Anna
Forma, Ewa
Bryś, Magdalena
Lipiński, Marek
Różański, Waldemar
Rate of positive urine culture and double–J catheters colonization on the basis of microorganism DNA analysis
title Rate of positive urine culture and double–J catheters colonization on the basis of microorganism DNA analysis
title_full Rate of positive urine culture and double–J catheters colonization on the basis of microorganism DNA analysis
title_fullStr Rate of positive urine culture and double–J catheters colonization on the basis of microorganism DNA analysis
title_full_unstemmed Rate of positive urine culture and double–J catheters colonization on the basis of microorganism DNA analysis
title_short Rate of positive urine culture and double–J catheters colonization on the basis of microorganism DNA analysis
title_sort rate of positive urine culture and double–j catheters colonization on the basis of microorganism dna analysis
topic Original Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4074722/
https://www.ncbi.nlm.nih.gov/pubmed/24982789
http://dx.doi.org/10.5173/ceju.2014.01.art18
work_keys_str_mv AT klisrafał rateofpositiveurinecultureanddoublejcatheterscolonizationonthebasisofmicroorganismdnaanalysis
AT szymkowiaksylwia rateofpositiveurinecultureanddoublejcatheterscolonizationonthebasisofmicroorganismdnaanalysis
AT madejadam rateofpositiveurinecultureanddoublejcatheterscolonizationonthebasisofmicroorganismdnaanalysis
AT blewniewskimariusz rateofpositiveurinecultureanddoublejcatheterscolonizationonthebasisofmicroorganismdnaanalysis
AT krzeslakanna rateofpositiveurinecultureanddoublejcatheterscolonizationonthebasisofmicroorganismdnaanalysis
AT formaewa rateofpositiveurinecultureanddoublejcatheterscolonizationonthebasisofmicroorganismdnaanalysis
AT brysmagdalena rateofpositiveurinecultureanddoublejcatheterscolonizationonthebasisofmicroorganismdnaanalysis
AT lipinskimarek rateofpositiveurinecultureanddoublejcatheterscolonizationonthebasisofmicroorganismdnaanalysis
AT rozanskiwaldemar rateofpositiveurinecultureanddoublejcatheterscolonizationonthebasisofmicroorganismdnaanalysis