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Electrophysiological characterization of male goldfish (Carassius auratus) ventral preoptic area neurons receiving olfactory inputs

Chemical communication via sex pheromones is critical for successful reproduction but the underlying neural mechanisms are not well-understood. The goldfish is a tractable model because sex pheromones have been well-characterized in this species. We used male goldfish forebrain explants in vitro and...

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Autores principales: Lado, Wudu E., Spanswick, David C., Lewis, John E., Trudeau, Vance L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4074913/
https://www.ncbi.nlm.nih.gov/pubmed/25071430
http://dx.doi.org/10.3389/fnins.2014.00185
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author Lado, Wudu E.
Spanswick, David C.
Lewis, John E.
Trudeau, Vance L.
author_facet Lado, Wudu E.
Spanswick, David C.
Lewis, John E.
Trudeau, Vance L.
author_sort Lado, Wudu E.
collection PubMed
description Chemical communication via sex pheromones is critical for successful reproduction but the underlying neural mechanisms are not well-understood. The goldfish is a tractable model because sex pheromones have been well-characterized in this species. We used male goldfish forebrain explants in vitro and performed whole-cell current clamp recordings from single neurons in the ventral preoptic area (vPOA) to characterize their membrane properties and synaptic inputs from the olfactory bulbs (OB). Principle component and cluster analyses based on intrinsic membrane properties of vPOA neurons (N = 107) revealed five (I–V) distinct cell groups. These cells displayed differences in their input resistance (R(input): I < II < IV < III = V), time constant (TC: I = II < IV < III = V), and threshold current (I(threshold): I > II = IV > III = V). Evidence from electrical stimulation of the OB and application of receptor antagonists suggests that vPOA neurons receive monosynaptic glutamatergic inputs via the medial olfactory tract, with connectivity varying among neuronal groups [I (24%), II (40%), III (0%), IV (34%), and V (2%)].
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spelling pubmed-40749132014-07-28 Electrophysiological characterization of male goldfish (Carassius auratus) ventral preoptic area neurons receiving olfactory inputs Lado, Wudu E. Spanswick, David C. Lewis, John E. Trudeau, Vance L. Front Neurosci Endocrinology Chemical communication via sex pheromones is critical for successful reproduction but the underlying neural mechanisms are not well-understood. The goldfish is a tractable model because sex pheromones have been well-characterized in this species. We used male goldfish forebrain explants in vitro and performed whole-cell current clamp recordings from single neurons in the ventral preoptic area (vPOA) to characterize their membrane properties and synaptic inputs from the olfactory bulbs (OB). Principle component and cluster analyses based on intrinsic membrane properties of vPOA neurons (N = 107) revealed five (I–V) distinct cell groups. These cells displayed differences in their input resistance (R(input): I < II < IV < III = V), time constant (TC: I = II < IV < III = V), and threshold current (I(threshold): I > II = IV > III = V). Evidence from electrical stimulation of the OB and application of receptor antagonists suggests that vPOA neurons receive monosynaptic glutamatergic inputs via the medial olfactory tract, with connectivity varying among neuronal groups [I (24%), II (40%), III (0%), IV (34%), and V (2%)]. Frontiers Media S.A. 2014-06-30 /pmc/articles/PMC4074913/ /pubmed/25071430 http://dx.doi.org/10.3389/fnins.2014.00185 Text en Copyright © 2014 Lado, Spanswick, Lewis and Trudeau. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Endocrinology
Lado, Wudu E.
Spanswick, David C.
Lewis, John E.
Trudeau, Vance L.
Electrophysiological characterization of male goldfish (Carassius auratus) ventral preoptic area neurons receiving olfactory inputs
title Electrophysiological characterization of male goldfish (Carassius auratus) ventral preoptic area neurons receiving olfactory inputs
title_full Electrophysiological characterization of male goldfish (Carassius auratus) ventral preoptic area neurons receiving olfactory inputs
title_fullStr Electrophysiological characterization of male goldfish (Carassius auratus) ventral preoptic area neurons receiving olfactory inputs
title_full_unstemmed Electrophysiological characterization of male goldfish (Carassius auratus) ventral preoptic area neurons receiving olfactory inputs
title_short Electrophysiological characterization of male goldfish (Carassius auratus) ventral preoptic area neurons receiving olfactory inputs
title_sort electrophysiological characterization of male goldfish (carassius auratus) ventral preoptic area neurons receiving olfactory inputs
topic Endocrinology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4074913/
https://www.ncbi.nlm.nih.gov/pubmed/25071430
http://dx.doi.org/10.3389/fnins.2014.00185
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