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Molecular Characterization of Watermelon Chlorotic Stunt Virus (WmCSV) from Palestine
The incidence of watermelon chlorotic stunt disease and molecular characterization of the Palestinian isolate of Watermelon chlorotic stunt virus (WmCSV-[PAL]) are described in this study. Symptomatic leaf samples obtained from watermelon Citrullus lanatus (Thunb.), and cucumber (Cucumis sativus L.)...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4074936/ https://www.ncbi.nlm.nih.gov/pubmed/24956181 http://dx.doi.org/10.3390/v6062444 |
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author | Ali-Shtayeh, Mohammed S. Jamous, Rana M. Mallah, Omar B. Abu-Zeitoun, Salam Y. |
author_facet | Ali-Shtayeh, Mohammed S. Jamous, Rana M. Mallah, Omar B. Abu-Zeitoun, Salam Y. |
author_sort | Ali-Shtayeh, Mohammed S. |
collection | PubMed |
description | The incidence of watermelon chlorotic stunt disease and molecular characterization of the Palestinian isolate of Watermelon chlorotic stunt virus (WmCSV-[PAL]) are described in this study. Symptomatic leaf samples obtained from watermelon Citrullus lanatus (Thunb.), and cucumber (Cucumis sativus L.) plants were tested for WmCSV-[PAL] infection by polymerase chain reaction (PCR) and Rolling Circle Amplification (RCA). Disease incidence ranged between 25%–98% in watermelon fields in the studied area, 77% of leaf samples collected from Jenin were found to be mixed infected with WmCSV-[PAL] and SLCV. The full-length DNA-A and DNA-B genomes of WmCSV-[PAL] were amplified and sequenced, and the sequences were deposited in the GenBank. Sequence analysis of virus genomes showed that DNA-A and DNA-B had 97.6%–99.42% and 93.16%–98.26% nucleotide identity with other virus isolates in the region, respectively. Sequence analysis also revealed that the Palestinian isolate of WmCSV shared the highest nucleotide identity with an isolate from Israel suggesting that the virus was introduced to Palestine from Israel. |
format | Online Article Text |
id | pubmed-4074936 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-40749362014-06-30 Molecular Characterization of Watermelon Chlorotic Stunt Virus (WmCSV) from Palestine Ali-Shtayeh, Mohammed S. Jamous, Rana M. Mallah, Omar B. Abu-Zeitoun, Salam Y. Viruses Article The incidence of watermelon chlorotic stunt disease and molecular characterization of the Palestinian isolate of Watermelon chlorotic stunt virus (WmCSV-[PAL]) are described in this study. Symptomatic leaf samples obtained from watermelon Citrullus lanatus (Thunb.), and cucumber (Cucumis sativus L.) plants were tested for WmCSV-[PAL] infection by polymerase chain reaction (PCR) and Rolling Circle Amplification (RCA). Disease incidence ranged between 25%–98% in watermelon fields in the studied area, 77% of leaf samples collected from Jenin were found to be mixed infected with WmCSV-[PAL] and SLCV. The full-length DNA-A and DNA-B genomes of WmCSV-[PAL] were amplified and sequenced, and the sequences were deposited in the GenBank. Sequence analysis of virus genomes showed that DNA-A and DNA-B had 97.6%–99.42% and 93.16%–98.26% nucleotide identity with other virus isolates in the region, respectively. Sequence analysis also revealed that the Palestinian isolate of WmCSV shared the highest nucleotide identity with an isolate from Israel suggesting that the virus was introduced to Palestine from Israel. MDPI 2014-06-20 /pmc/articles/PMC4074936/ /pubmed/24956181 http://dx.doi.org/10.3390/v6062444 Text en © 2014 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/). |
spellingShingle | Article Ali-Shtayeh, Mohammed S. Jamous, Rana M. Mallah, Omar B. Abu-Zeitoun, Salam Y. Molecular Characterization of Watermelon Chlorotic Stunt Virus (WmCSV) from Palestine |
title | Molecular Characterization of Watermelon Chlorotic Stunt Virus (WmCSV) from Palestine |
title_full | Molecular Characterization of Watermelon Chlorotic Stunt Virus (WmCSV) from Palestine |
title_fullStr | Molecular Characterization of Watermelon Chlorotic Stunt Virus (WmCSV) from Palestine |
title_full_unstemmed | Molecular Characterization of Watermelon Chlorotic Stunt Virus (WmCSV) from Palestine |
title_short | Molecular Characterization of Watermelon Chlorotic Stunt Virus (WmCSV) from Palestine |
title_sort | molecular characterization of watermelon chlorotic stunt virus (wmcsv) from palestine |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4074936/ https://www.ncbi.nlm.nih.gov/pubmed/24956181 http://dx.doi.org/10.3390/v6062444 |
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