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Trypan blue exclusion assay by flow cytometry
Dye exclusion tests are used to determine the number of live and dead cells. These assays are based on the principle that intact plasma membranes in live cells exclude specific dyes, whereas dead cells do not. Although widely used, the trypan blue (TB) exclusion assay has limitations. The dye can be...
Autores principales: | Avelar-Freitas, B.A., Almeida, V.G., Pinto, M.C.X., Mourão, F.A.G., Massensini, A.R., Martins-Filho, O.A., Rocha-Vieira, E., Brito-Melo, G.E.A. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Associação Brasileira de Divulgação Científica
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4075294/ https://www.ncbi.nlm.nih.gov/pubmed/24652322 http://dx.doi.org/10.1590/1414-431X20143437 |
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