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Evaluation of a Chromogenic Culture Medium for the Detection of Clostridium difficile

PURPOSE: Clostridium difficile (C. difficile) is an important cause of nosocomial diarrhea. Diagnostic methods for detection of C. difficile infection (CDI) are shifting to molecular techniques, which are faster and more sensitive than conventional methods. Although recent advances in these methods...

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Autores principales: Yang, John Jeongseok, Nam, You Sun, Kim, Min Jin, Cho, Sun Young, You, Eunkyung, Soh, Yun Soo, Lee, Hee Joo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Yonsei University College of Medicine 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4075405/
https://www.ncbi.nlm.nih.gov/pubmed/24954329
http://dx.doi.org/10.3349/ymj.2014.55.4.994
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author Yang, John Jeongseok
Nam, You Sun
Kim, Min Jin
Cho, Sun Young
You, Eunkyung
Soh, Yun Soo
Lee, Hee Joo
author_facet Yang, John Jeongseok
Nam, You Sun
Kim, Min Jin
Cho, Sun Young
You, Eunkyung
Soh, Yun Soo
Lee, Hee Joo
author_sort Yang, John Jeongseok
collection PubMed
description PURPOSE: Clostridium difficile (C. difficile) is an important cause of nosocomial diarrhea. Diagnostic methods for detection of C. difficile infection (CDI) are shifting to molecular techniques, which are faster and more sensitive than conventional methods. Although recent advances in these methods have been made in terms of their cost-benefit, ease of use, and turnaround time, anaerobic culture remains an important method for detection of CDI. MATERIALS AND METHODS: In efforts to evaluate a novel chromogenic medium for the detection of C. difficile (chromID CD agar), 289 fecal specimens were analyzed using two other culture media of blood agar and cycloserine-cefoxitin-fructose-egg yolk agar while enzyme immunosorbent assay and polymerase chain reaction-based assay were used for toxin detection. RESULTS: ChromID showed the highest detection rate among the three culture media. Both positive rate and sensitivity were higher from chromID than other culture media. ChromID was better at detecting toxin producing C. difficile at 24 h and showed the highest detection rate at both 24 h and 48 h. CONCLUSION: Simultaneous use of toxin assay and anaerobic culture has been considered as the most accurate and sensitive diagnostic approach of CDI. Utilization of a more rapid and sensitive chromogenic medium will aid in the dianogsis of CDI.
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spelling pubmed-40754052014-07-01 Evaluation of a Chromogenic Culture Medium for the Detection of Clostridium difficile Yang, John Jeongseok Nam, You Sun Kim, Min Jin Cho, Sun Young You, Eunkyung Soh, Yun Soo Lee, Hee Joo Yonsei Med J Original Article PURPOSE: Clostridium difficile (C. difficile) is an important cause of nosocomial diarrhea. Diagnostic methods for detection of C. difficile infection (CDI) are shifting to molecular techniques, which are faster and more sensitive than conventional methods. Although recent advances in these methods have been made in terms of their cost-benefit, ease of use, and turnaround time, anaerobic culture remains an important method for detection of CDI. MATERIALS AND METHODS: In efforts to evaluate a novel chromogenic medium for the detection of C. difficile (chromID CD agar), 289 fecal specimens were analyzed using two other culture media of blood agar and cycloserine-cefoxitin-fructose-egg yolk agar while enzyme immunosorbent assay and polymerase chain reaction-based assay were used for toxin detection. RESULTS: ChromID showed the highest detection rate among the three culture media. Both positive rate and sensitivity were higher from chromID than other culture media. ChromID was better at detecting toxin producing C. difficile at 24 h and showed the highest detection rate at both 24 h and 48 h. CONCLUSION: Simultaneous use of toxin assay and anaerobic culture has been considered as the most accurate and sensitive diagnostic approach of CDI. Utilization of a more rapid and sensitive chromogenic medium will aid in the dianogsis of CDI. Yonsei University College of Medicine 2014-07-01 2014-06-13 /pmc/articles/PMC4075405/ /pubmed/24954329 http://dx.doi.org/10.3349/ymj.2014.55.4.994 Text en © Copyright: Yonsei University College of Medicine 2014 http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Yang, John Jeongseok
Nam, You Sun
Kim, Min Jin
Cho, Sun Young
You, Eunkyung
Soh, Yun Soo
Lee, Hee Joo
Evaluation of a Chromogenic Culture Medium for the Detection of Clostridium difficile
title Evaluation of a Chromogenic Culture Medium for the Detection of Clostridium difficile
title_full Evaluation of a Chromogenic Culture Medium for the Detection of Clostridium difficile
title_fullStr Evaluation of a Chromogenic Culture Medium for the Detection of Clostridium difficile
title_full_unstemmed Evaluation of a Chromogenic Culture Medium for the Detection of Clostridium difficile
title_short Evaluation of a Chromogenic Culture Medium for the Detection of Clostridium difficile
title_sort evaluation of a chromogenic culture medium for the detection of clostridium difficile
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4075405/
https://www.ncbi.nlm.nih.gov/pubmed/24954329
http://dx.doi.org/10.3349/ymj.2014.55.4.994
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