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Antioxidant, analgesic and anti-inflammatory activities of the methanolic extract of Piper betle leaves
Objective: The present study was designed to evaluate the antioxidant, analgesic, and anti-inflammatory activities of the methanolic extract of Piper betle leaves (MPBL). Materials and Methods: MPBL was evaluated for anti-inflammatory activity using carrageenan-induced hind paw edema model. Analgesi...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Mashhad University of Medical Sciences
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4075698/ https://www.ncbi.nlm.nih.gov/pubmed/25050265 |
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author | Alam, Badrul Akter, Fahima Parvin, Nahida Sharmin Pia, Rashna Akter, Sharmin Chowdhury, Jesmin Sifath-E-Jahan, Kazi Haque, Ekramul |
author_facet | Alam, Badrul Akter, Fahima Parvin, Nahida Sharmin Pia, Rashna Akter, Sharmin Chowdhury, Jesmin Sifath-E-Jahan, Kazi Haque, Ekramul |
author_sort | Alam, Badrul |
collection | PubMed |
description | Objective: The present study was designed to evaluate the antioxidant, analgesic, and anti-inflammatory activities of the methanolic extract of Piper betle leaves (MPBL). Materials and Methods: MPBL was evaluated for anti-inflammatory activity using carrageenan-induced hind paw edema model. Analgesic activity of MPBL was evaluated by hot plate, writhing, and formalin tests. Total phenolic and flavonoids content, total antioxidant activity, scavenging of 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, peroxynitrate (ONOO) as well as inhibition of total ROS generation, and assessment of reducing power were used to evaluate antioxidant potential of MPBL. Results: The extract of MPBL, at the dose of 100 and 200 mg/kg, produced a significant (p<0.05) increase in pain threshold in hot plate method whereas significantly (p<0.05) reduced the writhing caused by acetic acid and the number of licks induced by formalin in a dose-dependent manner. The same ranges of doses of MPBL caused significant (p<0.05) inhibition of carrageenan-induced paw edema after 4 h in a dose-dependent manner. In DPPH, ONOO(-), and total ROS scavenging method, MPBL showed good antioxidant potentiality with the IC(50) value of 16.33±1.02, 25.16±0.61 , and 41.72±0.48 µg/ml, respectively with a significant (p<0.05) good reducing power. Conclusion: The findings of the study suggested that MPBL has strong analgesic, anti-inflammatory, and antioxidant effects, conforming the traditional use of this plant for inflammatory pain alleviation to its antioxidant potentiality. |
format | Online Article Text |
id | pubmed-4075698 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Mashhad University of Medical Sciences |
record_format | MEDLINE/PubMed |
spelling | pubmed-40756982014-07-21 Antioxidant, analgesic and anti-inflammatory activities of the methanolic extract of Piper betle leaves Alam, Badrul Akter, Fahima Parvin, Nahida Sharmin Pia, Rashna Akter, Sharmin Chowdhury, Jesmin Sifath-E-Jahan, Kazi Haque, Ekramul Avicenna J Phytomed Original Article Objective: The present study was designed to evaluate the antioxidant, analgesic, and anti-inflammatory activities of the methanolic extract of Piper betle leaves (MPBL). Materials and Methods: MPBL was evaluated for anti-inflammatory activity using carrageenan-induced hind paw edema model. Analgesic activity of MPBL was evaluated by hot plate, writhing, and formalin tests. Total phenolic and flavonoids content, total antioxidant activity, scavenging of 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, peroxynitrate (ONOO) as well as inhibition of total ROS generation, and assessment of reducing power were used to evaluate antioxidant potential of MPBL. Results: The extract of MPBL, at the dose of 100 and 200 mg/kg, produced a significant (p<0.05) increase in pain threshold in hot plate method whereas significantly (p<0.05) reduced the writhing caused by acetic acid and the number of licks induced by formalin in a dose-dependent manner. The same ranges of doses of MPBL caused significant (p<0.05) inhibition of carrageenan-induced paw edema after 4 h in a dose-dependent manner. In DPPH, ONOO(-), and total ROS scavenging method, MPBL showed good antioxidant potentiality with the IC(50) value of 16.33±1.02, 25.16±0.61 , and 41.72±0.48 µg/ml, respectively with a significant (p<0.05) good reducing power. Conclusion: The findings of the study suggested that MPBL has strong analgesic, anti-inflammatory, and antioxidant effects, conforming the traditional use of this plant for inflammatory pain alleviation to its antioxidant potentiality. Mashhad University of Medical Sciences 2013 /pmc/articles/PMC4075698/ /pubmed/25050265 Text en © 2013: Avicenna Journal of Phytomedicine This is an Open Access article distributed under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Alam, Badrul Akter, Fahima Parvin, Nahida Sharmin Pia, Rashna Akter, Sharmin Chowdhury, Jesmin Sifath-E-Jahan, Kazi Haque, Ekramul Antioxidant, analgesic and anti-inflammatory activities of the methanolic extract of Piper betle leaves |
title | Antioxidant, analgesic and anti-inflammatory activities of the methanolic extract of Piper betle leaves |
title_full | Antioxidant, analgesic and anti-inflammatory activities of the methanolic extract of Piper betle leaves |
title_fullStr | Antioxidant, analgesic and anti-inflammatory activities of the methanolic extract of Piper betle leaves |
title_full_unstemmed | Antioxidant, analgesic and anti-inflammatory activities of the methanolic extract of Piper betle leaves |
title_short | Antioxidant, analgesic and anti-inflammatory activities of the methanolic extract of Piper betle leaves |
title_sort | antioxidant, analgesic and anti-inflammatory activities of the methanolic extract of piper betle leaves |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4075698/ https://www.ncbi.nlm.nih.gov/pubmed/25050265 |
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