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Effects of Foeniculum vulgare ethanol extract on osteogenesis in human mecenchymal stem cells

Objective: Osteoporosis or silent disease is a major bone disorder in elderly women in current century. Estrogen has an important role in osteogenesis and prevention of bone fractures. Hormone replacement therapy (HRT) is usually accompanied by such effects as breast and ovary cancers. Thus, there i...

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Autores principales: Mahmoudi, Zahra, Soleimani, Masoud, saidi, Abbas, Khamisipour, Gholamreza, Azizsoltani, Arezoo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Mashhad University of Medical Sciences 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4075705/
https://www.ncbi.nlm.nih.gov/pubmed/25050267
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author Mahmoudi, Zahra
Soleimani, Masoud
saidi, Abbas
Khamisipour, Gholamreza
Azizsoltani, Arezoo
author_facet Mahmoudi, Zahra
Soleimani, Masoud
saidi, Abbas
Khamisipour, Gholamreza
Azizsoltani, Arezoo
author_sort Mahmoudi, Zahra
collection PubMed
description Objective: Osteoporosis or silent disease is a major bone disorder in elderly women in current century. Estrogen has an important role in osteogenesis and prevention of bone fractures. Hormone replacement therapy (HRT) is usually accompanied by such effects as breast and ovary cancers. Thus, there is an increasing demand for replacement with plant phytoestrogens. This study is focused on determining the effects of Foeniculum vulgare extract on proliferation and osteogenesis progress in human mesenchymal stem cells. Materials and Methods: Human mesenchymal stem cells were isolated and treated with different amount of plant extracts (0.5 to 100 µg/ml). Extract cytotoxicity was measured using MTT assay. The alkaline phosphatase enzyme activity was measured to evaluate the differentiation progress. Results: Results of MTT assay and alkaline phosphatase activity showed that Foeniculum vulgare extract, at range of 5 to 50 µg/ml, may positively affect cell proliferation and mineralization. The most proliferation and enzyme activity were seen with dose of 5 µg/ml. Conclusions: Foeniculum vulgare has been used in Iranian folk medicine for many years. Our in vitro study showed that Foeniculum vulgare extract has osteoprotective effects.
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spelling pubmed-40757052014-07-21 Effects of Foeniculum vulgare ethanol extract on osteogenesis in human mecenchymal stem cells Mahmoudi, Zahra Soleimani, Masoud saidi, Abbas Khamisipour, Gholamreza Azizsoltani, Arezoo Avicenna J Phytomed Original Article Objective: Osteoporosis or silent disease is a major bone disorder in elderly women in current century. Estrogen has an important role in osteogenesis and prevention of bone fractures. Hormone replacement therapy (HRT) is usually accompanied by such effects as breast and ovary cancers. Thus, there is an increasing demand for replacement with plant phytoestrogens. This study is focused on determining the effects of Foeniculum vulgare extract on proliferation and osteogenesis progress in human mesenchymal stem cells. Materials and Methods: Human mesenchymal stem cells were isolated and treated with different amount of plant extracts (0.5 to 100 µg/ml). Extract cytotoxicity was measured using MTT assay. The alkaline phosphatase enzyme activity was measured to evaluate the differentiation progress. Results: Results of MTT assay and alkaline phosphatase activity showed that Foeniculum vulgare extract, at range of 5 to 50 µg/ml, may positively affect cell proliferation and mineralization. The most proliferation and enzyme activity were seen with dose of 5 µg/ml. Conclusions: Foeniculum vulgare has been used in Iranian folk medicine for many years. Our in vitro study showed that Foeniculum vulgare extract has osteoprotective effects. Mashhad University of Medical Sciences 2013 /pmc/articles/PMC4075705/ /pubmed/25050267 Text en © 2013: Avicenna Journal of Phytomedicine This is an Open Access article distributed under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Mahmoudi, Zahra
Soleimani, Masoud
saidi, Abbas
Khamisipour, Gholamreza
Azizsoltani, Arezoo
Effects of Foeniculum vulgare ethanol extract on osteogenesis in human mecenchymal stem cells
title Effects of Foeniculum vulgare ethanol extract on osteogenesis in human mecenchymal stem cells
title_full Effects of Foeniculum vulgare ethanol extract on osteogenesis in human mecenchymal stem cells
title_fullStr Effects of Foeniculum vulgare ethanol extract on osteogenesis in human mecenchymal stem cells
title_full_unstemmed Effects of Foeniculum vulgare ethanol extract on osteogenesis in human mecenchymal stem cells
title_short Effects of Foeniculum vulgare ethanol extract on osteogenesis in human mecenchymal stem cells
title_sort effects of foeniculum vulgare ethanol extract on osteogenesis in human mecenchymal stem cells
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4075705/
https://www.ncbi.nlm.nih.gov/pubmed/25050267
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