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Effect of tramadol on lung injury induced by skeletal muscle ischemia-reperfusion: an experimental study

OBJECTIVE: To determine whether tramadol has a protective effect against lung injury induced by skeletal muscle ischemia-reperfusion. METHODS: Twenty Wistar male rats were allocated to one of two groups: ischemia-reperfusion (IR) and ischemia-reperfusion + tramadol (IR+T). The animals were anestheti...

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Autores principales: Takhtfooladi, Mohammad Ashrafzadeh, Jahanshahi, Amirali, Sotoudeh, Amir, Jahanshahi, Gholamreza, Takhtfooladi, Hamed Ashrafzadeh, Aslani, Kimia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Sociedade Brasileira de Pneumologia e Tisiologia 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4075874/
https://www.ncbi.nlm.nih.gov/pubmed/24068264
http://dx.doi.org/10.1590/S1806-37132013000400006
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author Takhtfooladi, Mohammad Ashrafzadeh
Jahanshahi, Amirali
Sotoudeh, Amir
Jahanshahi, Gholamreza
Takhtfooladi, Hamed Ashrafzadeh
Aslani, Kimia
author_facet Takhtfooladi, Mohammad Ashrafzadeh
Jahanshahi, Amirali
Sotoudeh, Amir
Jahanshahi, Gholamreza
Takhtfooladi, Hamed Ashrafzadeh
Aslani, Kimia
author_sort Takhtfooladi, Mohammad Ashrafzadeh
collection PubMed
description OBJECTIVE: To determine whether tramadol has a protective effect against lung injury induced by skeletal muscle ischemia-reperfusion. METHODS: Twenty Wistar male rats were allocated to one of two groups: ischemia-reperfusion (IR) and ischemia-reperfusion + tramadol (IR+T). The animals were anesthetized with intramuscular injections of ketamine and xylazine (50 mg/kg and 10 mg/kg, respectively). All of the animals underwent 2-h ischemia by occlusion of the femoral artery and 24-h reperfusion. Prior to the occlusion of the femoral artery, 250 IU heparin were administered via the jugular vein in order to prevent clotting. The rats in the IR+T group were treated with tramadol (20 mg/kg i.v.) immediately before reperfusion. After the reperfusion period, the animals were euthanized with pentobarbital (300 mg/kg i.p.), the lungs were carefully removed, and specimens were properly prepared for histopathological and biochemical studies. RESULTS: Myeloperoxidase activity and nitric oxide levels were significantly higher in the IR group than in the IR+T group (p = 0.001 for both). Histological abnormalities, such as intra-alveolar edema, intra-alveolar hemorrhage, and neutrophil infiltration, were significantly more common in the IR group than in the IR+T group. CONCLUSIONS: On the basis of our histological and biochemical findings, we conclude that tramadol prevents lung tissue injury after skeletal muscle ischemia-reperfusion.
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spelling pubmed-40758742014-07-16 Effect of tramadol on lung injury induced by skeletal muscle ischemia-reperfusion: an experimental study Takhtfooladi, Mohammad Ashrafzadeh Jahanshahi, Amirali Sotoudeh, Amir Jahanshahi, Gholamreza Takhtfooladi, Hamed Ashrafzadeh Aslani, Kimia J Bras Pneumol Original Articles OBJECTIVE: To determine whether tramadol has a protective effect against lung injury induced by skeletal muscle ischemia-reperfusion. METHODS: Twenty Wistar male rats were allocated to one of two groups: ischemia-reperfusion (IR) and ischemia-reperfusion + tramadol (IR+T). The animals were anesthetized with intramuscular injections of ketamine and xylazine (50 mg/kg and 10 mg/kg, respectively). All of the animals underwent 2-h ischemia by occlusion of the femoral artery and 24-h reperfusion. Prior to the occlusion of the femoral artery, 250 IU heparin were administered via the jugular vein in order to prevent clotting. The rats in the IR+T group were treated with tramadol (20 mg/kg i.v.) immediately before reperfusion. After the reperfusion period, the animals were euthanized with pentobarbital (300 mg/kg i.p.), the lungs were carefully removed, and specimens were properly prepared for histopathological and biochemical studies. RESULTS: Myeloperoxidase activity and nitric oxide levels were significantly higher in the IR group than in the IR+T group (p = 0.001 for both). Histological abnormalities, such as intra-alveolar edema, intra-alveolar hemorrhage, and neutrophil infiltration, were significantly more common in the IR group than in the IR+T group. CONCLUSIONS: On the basis of our histological and biochemical findings, we conclude that tramadol prevents lung tissue injury after skeletal muscle ischemia-reperfusion. Sociedade Brasileira de Pneumologia e Tisiologia 2013 /pmc/articles/PMC4075874/ /pubmed/24068264 http://dx.doi.org/10.1590/S1806-37132013000400006 Text en http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Articles
Takhtfooladi, Mohammad Ashrafzadeh
Jahanshahi, Amirali
Sotoudeh, Amir
Jahanshahi, Gholamreza
Takhtfooladi, Hamed Ashrafzadeh
Aslani, Kimia
Effect of tramadol on lung injury induced by skeletal muscle ischemia-reperfusion: an experimental study
title Effect of tramadol on lung injury induced by skeletal muscle ischemia-reperfusion: an experimental study
title_full Effect of tramadol on lung injury induced by skeletal muscle ischemia-reperfusion: an experimental study
title_fullStr Effect of tramadol on lung injury induced by skeletal muscle ischemia-reperfusion: an experimental study
title_full_unstemmed Effect of tramadol on lung injury induced by skeletal muscle ischemia-reperfusion: an experimental study
title_short Effect of tramadol on lung injury induced by skeletal muscle ischemia-reperfusion: an experimental study
title_sort effect of tramadol on lung injury induced by skeletal muscle ischemia-reperfusion: an experimental study
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4075874/
https://www.ncbi.nlm.nih.gov/pubmed/24068264
http://dx.doi.org/10.1590/S1806-37132013000400006
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