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Effect of tramadol on lung injury induced by skeletal muscle ischemia-reperfusion: an experimental study
OBJECTIVE: To determine whether tramadol has a protective effect against lung injury induced by skeletal muscle ischemia-reperfusion. METHODS: Twenty Wistar male rats were allocated to one of two groups: ischemia-reperfusion (IR) and ischemia-reperfusion + tramadol (IR+T). The animals were anestheti...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Sociedade Brasileira de Pneumologia e Tisiologia
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4075874/ https://www.ncbi.nlm.nih.gov/pubmed/24068264 http://dx.doi.org/10.1590/S1806-37132013000400006 |
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author | Takhtfooladi, Mohammad Ashrafzadeh Jahanshahi, Amirali Sotoudeh, Amir Jahanshahi, Gholamreza Takhtfooladi, Hamed Ashrafzadeh Aslani, Kimia |
author_facet | Takhtfooladi, Mohammad Ashrafzadeh Jahanshahi, Amirali Sotoudeh, Amir Jahanshahi, Gholamreza Takhtfooladi, Hamed Ashrafzadeh Aslani, Kimia |
author_sort | Takhtfooladi, Mohammad Ashrafzadeh |
collection | PubMed |
description | OBJECTIVE: To determine whether tramadol has a protective effect against lung injury induced by skeletal muscle ischemia-reperfusion. METHODS: Twenty Wistar male rats were allocated to one of two groups: ischemia-reperfusion (IR) and ischemia-reperfusion + tramadol (IR+T). The animals were anesthetized with intramuscular injections of ketamine and xylazine (50 mg/kg and 10 mg/kg, respectively). All of the animals underwent 2-h ischemia by occlusion of the femoral artery and 24-h reperfusion. Prior to the occlusion of the femoral artery, 250 IU heparin were administered via the jugular vein in order to prevent clotting. The rats in the IR+T group were treated with tramadol (20 mg/kg i.v.) immediately before reperfusion. After the reperfusion period, the animals were euthanized with pentobarbital (300 mg/kg i.p.), the lungs were carefully removed, and specimens were properly prepared for histopathological and biochemical studies. RESULTS: Myeloperoxidase activity and nitric oxide levels were significantly higher in the IR group than in the IR+T group (p = 0.001 for both). Histological abnormalities, such as intra-alveolar edema, intra-alveolar hemorrhage, and neutrophil infiltration, were significantly more common in the IR group than in the IR+T group. CONCLUSIONS: On the basis of our histological and biochemical findings, we conclude that tramadol prevents lung tissue injury after skeletal muscle ischemia-reperfusion. |
format | Online Article Text |
id | pubmed-4075874 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Sociedade Brasileira de Pneumologia e Tisiologia |
record_format | MEDLINE/PubMed |
spelling | pubmed-40758742014-07-16 Effect of tramadol on lung injury induced by skeletal muscle ischemia-reperfusion: an experimental study Takhtfooladi, Mohammad Ashrafzadeh Jahanshahi, Amirali Sotoudeh, Amir Jahanshahi, Gholamreza Takhtfooladi, Hamed Ashrafzadeh Aslani, Kimia J Bras Pneumol Original Articles OBJECTIVE: To determine whether tramadol has a protective effect against lung injury induced by skeletal muscle ischemia-reperfusion. METHODS: Twenty Wistar male rats were allocated to one of two groups: ischemia-reperfusion (IR) and ischemia-reperfusion + tramadol (IR+T). The animals were anesthetized with intramuscular injections of ketamine and xylazine (50 mg/kg and 10 mg/kg, respectively). All of the animals underwent 2-h ischemia by occlusion of the femoral artery and 24-h reperfusion. Prior to the occlusion of the femoral artery, 250 IU heparin were administered via the jugular vein in order to prevent clotting. The rats in the IR+T group were treated with tramadol (20 mg/kg i.v.) immediately before reperfusion. After the reperfusion period, the animals were euthanized with pentobarbital (300 mg/kg i.p.), the lungs were carefully removed, and specimens were properly prepared for histopathological and biochemical studies. RESULTS: Myeloperoxidase activity and nitric oxide levels were significantly higher in the IR group than in the IR+T group (p = 0.001 for both). Histological abnormalities, such as intra-alveolar edema, intra-alveolar hemorrhage, and neutrophil infiltration, were significantly more common in the IR group than in the IR+T group. CONCLUSIONS: On the basis of our histological and biochemical findings, we conclude that tramadol prevents lung tissue injury after skeletal muscle ischemia-reperfusion. Sociedade Brasileira de Pneumologia e Tisiologia 2013 /pmc/articles/PMC4075874/ /pubmed/24068264 http://dx.doi.org/10.1590/S1806-37132013000400006 Text en http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Articles Takhtfooladi, Mohammad Ashrafzadeh Jahanshahi, Amirali Sotoudeh, Amir Jahanshahi, Gholamreza Takhtfooladi, Hamed Ashrafzadeh Aslani, Kimia Effect of tramadol on lung injury induced by skeletal muscle ischemia-reperfusion: an experimental study |
title | Effect of tramadol on lung injury induced by skeletal
muscle ischemia-reperfusion: an experimental study
|
title_full | Effect of tramadol on lung injury induced by skeletal
muscle ischemia-reperfusion: an experimental study
|
title_fullStr | Effect of tramadol on lung injury induced by skeletal
muscle ischemia-reperfusion: an experimental study
|
title_full_unstemmed | Effect of tramadol on lung injury induced by skeletal
muscle ischemia-reperfusion: an experimental study
|
title_short | Effect of tramadol on lung injury induced by skeletal
muscle ischemia-reperfusion: an experimental study
|
title_sort | effect of tramadol on lung injury induced by skeletal
muscle ischemia-reperfusion: an experimental study |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4075874/ https://www.ncbi.nlm.nih.gov/pubmed/24068264 http://dx.doi.org/10.1590/S1806-37132013000400006 |
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