A General NMR-Based Strategy for the in Situ Characterization of Sugar-Nucleotide-Dependent Biosynthetic Pathways

[Image: see text] A simple method for the study of sugar-nucleotide-dependent multienzyme cascades is highlighted where the use of selectively (13)C-labeled sugar nucleotides and inverse (13)C detection NMR offers fast, direct detection and quantification of reactants and products and circumvents th...

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Autores principales: Singh, Shanteri, Peltier-Pain, Pauline, Tonelli, Marco, Thorson, Jon S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2014
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4075999/
https://www.ncbi.nlm.nih.gov/pubmed/24911465
http://dx.doi.org/10.1021/ol501241a
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author Singh, Shanteri
Peltier-Pain, Pauline
Tonelli, Marco
Thorson, Jon S.
author_facet Singh, Shanteri
Peltier-Pain, Pauline
Tonelli, Marco
Thorson, Jon S.
author_sort Singh, Shanteri
collection PubMed
description [Image: see text] A simple method for the study of sugar-nucleotide-dependent multienzyme cascades is highlighted where the use of selectively (13)C-labeled sugar nucleotides and inverse (13)C detection NMR offers fast, direct detection and quantification of reactants and products and circumvents the need for chromatographic separation. The utility of the method has been demonstrated by characterizing four previously uncharacterized sugar nucleotide biosynthetic enzymes involved in calicheamicin biosynthesis.
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spelling pubmed-40759992015-06-09 A General NMR-Based Strategy for the in Situ Characterization of Sugar-Nucleotide-Dependent Biosynthetic Pathways Singh, Shanteri Peltier-Pain, Pauline Tonelli, Marco Thorson, Jon S. Org Lett [Image: see text] A simple method for the study of sugar-nucleotide-dependent multienzyme cascades is highlighted where the use of selectively (13)C-labeled sugar nucleotides and inverse (13)C detection NMR offers fast, direct detection and quantification of reactants and products and circumvents the need for chromatographic separation. The utility of the method has been demonstrated by characterizing four previously uncharacterized sugar nucleotide biosynthetic enzymes involved in calicheamicin biosynthesis. American Chemical Society 2014-06-09 2014-06-20 /pmc/articles/PMC4075999/ /pubmed/24911465 http://dx.doi.org/10.1021/ol501241a Text en Copyright © 2014 American Chemical Society Terms of Use (http://pubs.acs.org/page/policy/authorchoice_termsofuse.html)
spellingShingle Singh, Shanteri
Peltier-Pain, Pauline
Tonelli, Marco
Thorson, Jon S.
A General NMR-Based Strategy for the in Situ Characterization of Sugar-Nucleotide-Dependent Biosynthetic Pathways
title A General NMR-Based Strategy for the in Situ Characterization of Sugar-Nucleotide-Dependent Biosynthetic Pathways
title_full A General NMR-Based Strategy for the in Situ Characterization of Sugar-Nucleotide-Dependent Biosynthetic Pathways
title_fullStr A General NMR-Based Strategy for the in Situ Characterization of Sugar-Nucleotide-Dependent Biosynthetic Pathways
title_full_unstemmed A General NMR-Based Strategy for the in Situ Characterization of Sugar-Nucleotide-Dependent Biosynthetic Pathways
title_short A General NMR-Based Strategy for the in Situ Characterization of Sugar-Nucleotide-Dependent Biosynthetic Pathways
title_sort general nmr-based strategy for the in situ characterization of sugar-nucleotide-dependent biosynthetic pathways
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4075999/
https://www.ncbi.nlm.nih.gov/pubmed/24911465
http://dx.doi.org/10.1021/ol501241a
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