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TAO1 kinase maintains chromosomal stability by facilitating proper congression of chromosomes
Chromosomal instability can arise from defects in chromosome–microtubule attachment. Using a variety of drug treatments, we show that TAO1 kinase is required for ensuring the normal congression of chromosomes. Depletion of TAO1 reduces the density of growing interphase and mitotic microtubules in hu...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Royal Society
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4077056/ https://www.ncbi.nlm.nih.gov/pubmed/24898139 http://dx.doi.org/10.1098/rsob.130108 |
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author | Shrestha, Roshan L. Tamura, Naoka Fries, Anna Levin, Nicolas Clark, Joanna Draviam, Viji M. |
author_facet | Shrestha, Roshan L. Tamura, Naoka Fries, Anna Levin, Nicolas Clark, Joanna Draviam, Viji M. |
author_sort | Shrestha, Roshan L. |
collection | PubMed |
description | Chromosomal instability can arise from defects in chromosome–microtubule attachment. Using a variety of drug treatments, we show that TAO1 kinase is required for ensuring the normal congression of chromosomes. Depletion of TAO1 reduces the density of growing interphase and mitotic microtubules in human cells, showing TAO1's role in controlling microtubule dynamics. We demonstrate the aneugenic nature of chromosome–microtubule attachment defects in TAO1-depleted cells using an error-correction assay. Our model further strengthens the emerging paradigm that microtubule regulatory pathways are important for resolving erroneous kinetochore–microtubule attachments and maintaining the integrity of the genome, regardless of the spindle checkpoint status. |
format | Online Article Text |
id | pubmed-4077056 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | The Royal Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-40770562014-07-14 TAO1 kinase maintains chromosomal stability by facilitating proper congression of chromosomes Shrestha, Roshan L. Tamura, Naoka Fries, Anna Levin, Nicolas Clark, Joanna Draviam, Viji M. Open Biol Research Chromosomal instability can arise from defects in chromosome–microtubule attachment. Using a variety of drug treatments, we show that TAO1 kinase is required for ensuring the normal congression of chromosomes. Depletion of TAO1 reduces the density of growing interphase and mitotic microtubules in human cells, showing TAO1's role in controlling microtubule dynamics. We demonstrate the aneugenic nature of chromosome–microtubule attachment defects in TAO1-depleted cells using an error-correction assay. Our model further strengthens the emerging paradigm that microtubule regulatory pathways are important for resolving erroneous kinetochore–microtubule attachments and maintaining the integrity of the genome, regardless of the spindle checkpoint status. The Royal Society 2014-06-04 /pmc/articles/PMC4077056/ /pubmed/24898139 http://dx.doi.org/10.1098/rsob.130108 Text en http://creativecommons.org/licenses/by/3.0/ © 2014 The Authors. Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/3.0/, which permits unrestricted use, provided the original author and source are credited. |
spellingShingle | Research Shrestha, Roshan L. Tamura, Naoka Fries, Anna Levin, Nicolas Clark, Joanna Draviam, Viji M. TAO1 kinase maintains chromosomal stability by facilitating proper congression of chromosomes |
title | TAO1 kinase maintains chromosomal stability by facilitating proper congression of chromosomes |
title_full | TAO1 kinase maintains chromosomal stability by facilitating proper congression of chromosomes |
title_fullStr | TAO1 kinase maintains chromosomal stability by facilitating proper congression of chromosomes |
title_full_unstemmed | TAO1 kinase maintains chromosomal stability by facilitating proper congression of chromosomes |
title_short | TAO1 kinase maintains chromosomal stability by facilitating proper congression of chromosomes |
title_sort | tao1 kinase maintains chromosomal stability by facilitating proper congression of chromosomes |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4077056/ https://www.ncbi.nlm.nih.gov/pubmed/24898139 http://dx.doi.org/10.1098/rsob.130108 |
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