Single-cell western blotting

To measure cell-to-cell variation in protein-mediated functions — a hallmark of biological processes — we developed an approach to conduct ~10(3) concurrent single-cell western blots (scWesterns) in ~4 hours. A microscope slide supporting a 30 µm-thick photoactive polyacrylamide gel enables western...

Descripción completa

Detalles Bibliográficos
Autores principales: Hughes, Alex J., Spelke, Dawn P., Xu, Zhuchen, Kang, Chi-Chih, Schaffer, David V., Herr, Amy E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2014
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4077215/
https://www.ncbi.nlm.nih.gov/pubmed/24880876
http://dx.doi.org/10.1038/nmeth.2992

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4077215/
https://www.ncbi.nlm.nih.gov/pubmed/24880876
http://dx.doi.org/10.1038/nmeth.2992

Ejemplares similares