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Single-cell western blotting
To measure cell-to-cell variation in protein-mediated functions — a hallmark of biological processes — we developed an approach to conduct ~10(3) concurrent single-cell western blots (scWesterns) in ~4 hours. A microscope slide supporting a 30 µm-thick photoactive polyacrylamide gel enables western...
Autores principales: | Hughes, Alex J., Spelke, Dawn P., Xu, Zhuchen, Kang, Chi-Chih, Schaffer, David V., Herr, Amy E. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4077215/ https://www.ncbi.nlm.nih.gov/pubmed/24880876 http://dx.doi.org/10.1038/nmeth.2992 |
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