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Manipulation of isolated brain nerve terminals by an external magnetic field using D-mannose-coated γ-Fe(2)O(3) nano-sized particles and assessment of their effects on glutamate transport
The manipulation of brain nerve terminals by an external magnetic field promises breakthroughs in nano-neurotechnology. D-Mannose-coated superparamagnetic nanoparticles were synthesized by coprecipitation of Fe(II) and Fe(III) salts followed by oxidation with sodium hypochlorite and addition of D-ma...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Beilstein-Institut
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4077395/ https://www.ncbi.nlm.nih.gov/pubmed/24991515 http://dx.doi.org/10.3762/bjnano.5.90 |
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author | Borisova, Tatiana Krisanova, Natalia Borуsov, Arsenii Sivko, Roman Ostapchenko, Ludmila Babic, Michal Horak, Daniel |
author_facet | Borisova, Tatiana Krisanova, Natalia Borуsov, Arsenii Sivko, Roman Ostapchenko, Ludmila Babic, Michal Horak, Daniel |
author_sort | Borisova, Tatiana |
collection | PubMed |
description | The manipulation of brain nerve terminals by an external magnetic field promises breakthroughs in nano-neurotechnology. D-Mannose-coated superparamagnetic nanoparticles were synthesized by coprecipitation of Fe(II) and Fe(III) salts followed by oxidation with sodium hypochlorite and addition of D-mannose. Effects of D-mannose-coated superparamagnetic maghemite (γ-Fe(2)O(3)) nanoparticles on key characteristics of the glutamatergic neurotransmission were analysed. Using radiolabeled L-[(14)C]glutamate, it was shown that D-mannose-coated γ-Fe(2)O(3) nanoparticles did not affect high-affinity Na(+)-dependent uptake, tonic release and the extracellular level of L-[(14)C]glutamate in isolated rat brain nerve terminals (synaptosomes). Also, the membrane potential of synaptosomes and acidification of synaptic vesicles was not changed as a result of the application of D-mannose-coated γ-Fe(2)O(3) nanoparticles. This was demonstrated with the potential-sensitive fluorescent dye rhodamine 6G and the pH-sensitive dye acridine orange. The study also focused on the analysis of the potential use of these nanoparticles for manipulation of nerve terminals by an external magnetic field. It was shown that more than 84.3 ± 5.0% of L-[(14)C]glutamate-loaded synaptosomes (1 mg of protein/mL) incubated for 5 min with D-mannose-coated γ-Fe(2)O(3) nanoparticles (250 µg/mL) moved to an area, in which the magnet (250 mT, gradient 5.5 Т/m) was applied compared to 33.5 ± 3.0% of the control and 48.6 ± 3.0% of samples that were treated with uncoated nanoparticles. Therefore, isolated brain nerve terminals can be easily manipulated by an external magnetic field using D-mannose-coated γ-Fe(2)O(3) nanoparticles, while the key characteristics of glutamatergic neurotransmission are not affected. In other words, functionally active synaptosomes labeled with D-mannose-coated γ-Fe(2)O(3) nanoparticles were obtained. |
format | Online Article Text |
id | pubmed-4077395 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Beilstein-Institut |
record_format | MEDLINE/PubMed |
spelling | pubmed-40773952014-07-02 Manipulation of isolated brain nerve terminals by an external magnetic field using D-mannose-coated γ-Fe(2)O(3) nano-sized particles and assessment of their effects on glutamate transport Borisova, Tatiana Krisanova, Natalia Borуsov, Arsenii Sivko, Roman Ostapchenko, Ludmila Babic, Michal Horak, Daniel Beilstein J Nanotechnol Full Research Paper The manipulation of brain nerve terminals by an external magnetic field promises breakthroughs in nano-neurotechnology. D-Mannose-coated superparamagnetic nanoparticles were synthesized by coprecipitation of Fe(II) and Fe(III) salts followed by oxidation with sodium hypochlorite and addition of D-mannose. Effects of D-mannose-coated superparamagnetic maghemite (γ-Fe(2)O(3)) nanoparticles on key characteristics of the glutamatergic neurotransmission were analysed. Using radiolabeled L-[(14)C]glutamate, it was shown that D-mannose-coated γ-Fe(2)O(3) nanoparticles did not affect high-affinity Na(+)-dependent uptake, tonic release and the extracellular level of L-[(14)C]glutamate in isolated rat brain nerve terminals (synaptosomes). Also, the membrane potential of synaptosomes and acidification of synaptic vesicles was not changed as a result of the application of D-mannose-coated γ-Fe(2)O(3) nanoparticles. This was demonstrated with the potential-sensitive fluorescent dye rhodamine 6G and the pH-sensitive dye acridine orange. The study also focused on the analysis of the potential use of these nanoparticles for manipulation of nerve terminals by an external magnetic field. It was shown that more than 84.3 ± 5.0% of L-[(14)C]glutamate-loaded synaptosomes (1 mg of protein/mL) incubated for 5 min with D-mannose-coated γ-Fe(2)O(3) nanoparticles (250 µg/mL) moved to an area, in which the magnet (250 mT, gradient 5.5 Т/m) was applied compared to 33.5 ± 3.0% of the control and 48.6 ± 3.0% of samples that were treated with uncoated nanoparticles. Therefore, isolated brain nerve terminals can be easily manipulated by an external magnetic field using D-mannose-coated γ-Fe(2)O(3) nanoparticles, while the key characteristics of glutamatergic neurotransmission are not affected. In other words, functionally active synaptosomes labeled with D-mannose-coated γ-Fe(2)O(3) nanoparticles were obtained. Beilstein-Institut 2014-06-04 /pmc/articles/PMC4077395/ /pubmed/24991515 http://dx.doi.org/10.3762/bjnano.5.90 Text en Copyright © 2014, Borisova et al. https://creativecommons.org/licenses/by/2.0https://www.beilstein-journals.org/bjnano/termsThis is an Open Access article under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The license is subject to the Beilstein Journal of Nanotechnology terms and conditions: (https://www.beilstein-journals.org/bjnano/terms) |
spellingShingle | Full Research Paper Borisova, Tatiana Krisanova, Natalia Borуsov, Arsenii Sivko, Roman Ostapchenko, Ludmila Babic, Michal Horak, Daniel Manipulation of isolated brain nerve terminals by an external magnetic field using D-mannose-coated γ-Fe(2)O(3) nano-sized particles and assessment of their effects on glutamate transport |
title | Manipulation of isolated brain nerve terminals by an external magnetic field using D-mannose-coated γ-Fe(2)O(3) nano-sized particles and assessment of their effects on glutamate transport |
title_full | Manipulation of isolated brain nerve terminals by an external magnetic field using D-mannose-coated γ-Fe(2)O(3) nano-sized particles and assessment of their effects on glutamate transport |
title_fullStr | Manipulation of isolated brain nerve terminals by an external magnetic field using D-mannose-coated γ-Fe(2)O(3) nano-sized particles and assessment of their effects on glutamate transport |
title_full_unstemmed | Manipulation of isolated brain nerve terminals by an external magnetic field using D-mannose-coated γ-Fe(2)O(3) nano-sized particles and assessment of their effects on glutamate transport |
title_short | Manipulation of isolated brain nerve terminals by an external magnetic field using D-mannose-coated γ-Fe(2)O(3) nano-sized particles and assessment of their effects on glutamate transport |
title_sort | manipulation of isolated brain nerve terminals by an external magnetic field using d-mannose-coated γ-fe(2)o(3) nano-sized particles and assessment of their effects on glutamate transport |
topic | Full Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4077395/ https://www.ncbi.nlm.nih.gov/pubmed/24991515 http://dx.doi.org/10.3762/bjnano.5.90 |
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