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Therapeutic Effects of Glutamic Acid in Piglets Challenged with Deoxynivalenol
The mycotoxin deoxynivalenol (DON), one of the most common food contaminants, primarily targets the gastrointestinal tract to affect animal and human health. This study was conducted to examine the protective function of glutamic acid on intestinal injury and oxidative stress caused by DON in piglet...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4077692/ https://www.ncbi.nlm.nih.gov/pubmed/24984001 http://dx.doi.org/10.1371/journal.pone.0100591 |
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author | Wu, Miaomiao Xiao, Hao Ren, Wenkai Yin, Jie Tan, Bie Liu, Gang Li, Lili Nyachoti, Charles Martin Xiong, Xia Wu, Guoyao |
author_facet | Wu, Miaomiao Xiao, Hao Ren, Wenkai Yin, Jie Tan, Bie Liu, Gang Li, Lili Nyachoti, Charles Martin Xiong, Xia Wu, Guoyao |
author_sort | Wu, Miaomiao |
collection | PubMed |
description | The mycotoxin deoxynivalenol (DON), one of the most common food contaminants, primarily targets the gastrointestinal tract to affect animal and human health. This study was conducted to examine the protective function of glutamic acid on intestinal injury and oxidative stress caused by DON in piglets. Twenty-eight piglets were assigned randomly into 4 dietary treatments (7 pigs/treatment): 1) uncontaminated control diet (NC), 2) NC+DON at 4 mg/kg (DON), 3) NC+2% glutamic acid (GLU), and 4) NC+2% glutamic acid + DON at 4 mg/kg (DG). At day 15, 30 and 37, blood samples were collected to determine serum concentrations of CAT (catalase), T-AOC (total antioxidant capacity), H(2)O(2) (hydrogen peroxide), NO (nitric oxide), MDA (maleic dialdehyde), DAO (diamine oxidase) and D-lactate. Intestinal morphology, and the activation of Akt/mTOR/4EBP1 signal pathway, as well as the concentrations of H(2)O(2), MDA, and DAO in kidney, liver and small intestine, were analyzed at day 37. Results showed that DON significantly (P<0.05) induced oxidative stress in piglets, while this stress was remarkably reduced with glutamic acid supplementation according to the change of oxidative parameters in blood and tissues. Meanwhile, DON caused obvious intestinal injury from microscopic observations and permeability indicators, which was alleviated by glutamic acid supplementation. Moreover, the inhibition of DON on Akt/mTOR/4EBP1 signal pathway was reduced by glutamic acid supplementation. Collectively, these data suggest that glutamic acid may be a useful nutritional regulator for DON-induced damage manifested as oxidative stress, intestinal injury and signaling inhibition. |
format | Online Article Text |
id | pubmed-4077692 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-40776922014-07-03 Therapeutic Effects of Glutamic Acid in Piglets Challenged with Deoxynivalenol Wu, Miaomiao Xiao, Hao Ren, Wenkai Yin, Jie Tan, Bie Liu, Gang Li, Lili Nyachoti, Charles Martin Xiong, Xia Wu, Guoyao PLoS One Research Article The mycotoxin deoxynivalenol (DON), one of the most common food contaminants, primarily targets the gastrointestinal tract to affect animal and human health. This study was conducted to examine the protective function of glutamic acid on intestinal injury and oxidative stress caused by DON in piglets. Twenty-eight piglets were assigned randomly into 4 dietary treatments (7 pigs/treatment): 1) uncontaminated control diet (NC), 2) NC+DON at 4 mg/kg (DON), 3) NC+2% glutamic acid (GLU), and 4) NC+2% glutamic acid + DON at 4 mg/kg (DG). At day 15, 30 and 37, blood samples were collected to determine serum concentrations of CAT (catalase), T-AOC (total antioxidant capacity), H(2)O(2) (hydrogen peroxide), NO (nitric oxide), MDA (maleic dialdehyde), DAO (diamine oxidase) and D-lactate. Intestinal morphology, and the activation of Akt/mTOR/4EBP1 signal pathway, as well as the concentrations of H(2)O(2), MDA, and DAO in kidney, liver and small intestine, were analyzed at day 37. Results showed that DON significantly (P<0.05) induced oxidative stress in piglets, while this stress was remarkably reduced with glutamic acid supplementation according to the change of oxidative parameters in blood and tissues. Meanwhile, DON caused obvious intestinal injury from microscopic observations and permeability indicators, which was alleviated by glutamic acid supplementation. Moreover, the inhibition of DON on Akt/mTOR/4EBP1 signal pathway was reduced by glutamic acid supplementation. Collectively, these data suggest that glutamic acid may be a useful nutritional regulator for DON-induced damage manifested as oxidative stress, intestinal injury and signaling inhibition. Public Library of Science 2014-07-01 /pmc/articles/PMC4077692/ /pubmed/24984001 http://dx.doi.org/10.1371/journal.pone.0100591 Text en © 2014 Wu et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Wu, Miaomiao Xiao, Hao Ren, Wenkai Yin, Jie Tan, Bie Liu, Gang Li, Lili Nyachoti, Charles Martin Xiong, Xia Wu, Guoyao Therapeutic Effects of Glutamic Acid in Piglets Challenged with Deoxynivalenol |
title | Therapeutic Effects of Glutamic Acid in Piglets Challenged with Deoxynivalenol |
title_full | Therapeutic Effects of Glutamic Acid in Piglets Challenged with Deoxynivalenol |
title_fullStr | Therapeutic Effects of Glutamic Acid in Piglets Challenged with Deoxynivalenol |
title_full_unstemmed | Therapeutic Effects of Glutamic Acid in Piglets Challenged with Deoxynivalenol |
title_short | Therapeutic Effects of Glutamic Acid in Piglets Challenged with Deoxynivalenol |
title_sort | therapeutic effects of glutamic acid in piglets challenged with deoxynivalenol |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4077692/ https://www.ncbi.nlm.nih.gov/pubmed/24984001 http://dx.doi.org/10.1371/journal.pone.0100591 |
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