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Partial Resistance of Carrot to Alternaria dauci Correlates with In Vitro Cultured Carrot Cell Resistance to Fungal Exudates

Although different mechanisms have been proposed in the recent years, plant pathogen partial resistance is still poorly understood. Components of the chemical warfare, including the production of plant defense compounds and plant resistance to pathogen-produced toxins, are likely to play a role. Tox...

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Autores principales: Lecomte, Mickaël, Hamama, Latifa, Voisine, Linda, Gatto, Julia, Hélesbeux, Jean-Jacques, Séraphin, Denis, Peña-Rodriguez, Luis M., Richomme, Pascal, Boedo, Cora, Yovanopoulos, Claire, Gyomlai, Melvina, Briard, Mathilde, Simoneau, Philippe, Poupard, Pascal, Berruyer, Romain
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4077726/
https://www.ncbi.nlm.nih.gov/pubmed/24983469
http://dx.doi.org/10.1371/journal.pone.0101008
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author Lecomte, Mickaël
Hamama, Latifa
Voisine, Linda
Gatto, Julia
Hélesbeux, Jean-Jacques
Séraphin, Denis
Peña-Rodriguez, Luis M.
Richomme, Pascal
Boedo, Cora
Yovanopoulos, Claire
Gyomlai, Melvina
Briard, Mathilde
Simoneau, Philippe
Poupard, Pascal
Berruyer, Romain
author_facet Lecomte, Mickaël
Hamama, Latifa
Voisine, Linda
Gatto, Julia
Hélesbeux, Jean-Jacques
Séraphin, Denis
Peña-Rodriguez, Luis M.
Richomme, Pascal
Boedo, Cora
Yovanopoulos, Claire
Gyomlai, Melvina
Briard, Mathilde
Simoneau, Philippe
Poupard, Pascal
Berruyer, Romain
author_sort Lecomte, Mickaël
collection PubMed
description Although different mechanisms have been proposed in the recent years, plant pathogen partial resistance is still poorly understood. Components of the chemical warfare, including the production of plant defense compounds and plant resistance to pathogen-produced toxins, are likely to play a role. Toxins are indeed recognized as important determinants of pathogenicity in necrotrophic fungi. Partial resistance based on quantitative resistance loci and linked to a pathogen-produced toxin has never been fully described. We tested this hypothesis using the Alternaria dauci – carrot pathosystem. Alternaria dauci, causing carrot leaf blight, is a necrotrophic fungus known to produce zinniol, a compound described as a non-host selective toxin. Embryogenic cellular cultures from carrot genotypes varying in resistance against A. dauci were confronted with zinniol at different concentrations or to fungal exudates (raw, organic or aqueous extracts). The plant response was analyzed through the measurement of cytoplasmic esterase activity, as a marker of cell viability, and the differentiation of somatic embryos in cellular cultures. A differential response to toxicity was demonstrated between susceptible and partially resistant genotypes, with a good correlation noted between the resistance to the fungus at the whole plant level and resistance at the cellular level to fungal exudates from raw and organic extracts. No toxic reaction of embryogenic cultures was observed after treatment with the aqueous extract or zinniol used at physiological concentration. Moreover, we did not detect zinniol in toxic fungal extracts by UHPLC analysis. These results suggest that strong phytotoxic compounds are present in the organic extract and remain to be characterized. Our results clearly show that carrot tolerance to A. dauci toxins is one component of its partial resistance.
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spelling pubmed-40777262014-07-03 Partial Resistance of Carrot to Alternaria dauci Correlates with In Vitro Cultured Carrot Cell Resistance to Fungal Exudates Lecomte, Mickaël Hamama, Latifa Voisine, Linda Gatto, Julia Hélesbeux, Jean-Jacques Séraphin, Denis Peña-Rodriguez, Luis M. Richomme, Pascal Boedo, Cora Yovanopoulos, Claire Gyomlai, Melvina Briard, Mathilde Simoneau, Philippe Poupard, Pascal Berruyer, Romain PLoS One Research Article Although different mechanisms have been proposed in the recent years, plant pathogen partial resistance is still poorly understood. Components of the chemical warfare, including the production of plant defense compounds and plant resistance to pathogen-produced toxins, are likely to play a role. Toxins are indeed recognized as important determinants of pathogenicity in necrotrophic fungi. Partial resistance based on quantitative resistance loci and linked to a pathogen-produced toxin has never been fully described. We tested this hypothesis using the Alternaria dauci – carrot pathosystem. Alternaria dauci, causing carrot leaf blight, is a necrotrophic fungus known to produce zinniol, a compound described as a non-host selective toxin. Embryogenic cellular cultures from carrot genotypes varying in resistance against A. dauci were confronted with zinniol at different concentrations or to fungal exudates (raw, organic or aqueous extracts). The plant response was analyzed through the measurement of cytoplasmic esterase activity, as a marker of cell viability, and the differentiation of somatic embryos in cellular cultures. A differential response to toxicity was demonstrated between susceptible and partially resistant genotypes, with a good correlation noted between the resistance to the fungus at the whole plant level and resistance at the cellular level to fungal exudates from raw and organic extracts. No toxic reaction of embryogenic cultures was observed after treatment with the aqueous extract or zinniol used at physiological concentration. Moreover, we did not detect zinniol in toxic fungal extracts by UHPLC analysis. These results suggest that strong phytotoxic compounds are present in the organic extract and remain to be characterized. Our results clearly show that carrot tolerance to A. dauci toxins is one component of its partial resistance. Public Library of Science 2014-07-01 /pmc/articles/PMC4077726/ /pubmed/24983469 http://dx.doi.org/10.1371/journal.pone.0101008 Text en © 2014 Lecomte et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Lecomte, Mickaël
Hamama, Latifa
Voisine, Linda
Gatto, Julia
Hélesbeux, Jean-Jacques
Séraphin, Denis
Peña-Rodriguez, Luis M.
Richomme, Pascal
Boedo, Cora
Yovanopoulos, Claire
Gyomlai, Melvina
Briard, Mathilde
Simoneau, Philippe
Poupard, Pascal
Berruyer, Romain
Partial Resistance of Carrot to Alternaria dauci Correlates with In Vitro Cultured Carrot Cell Resistance to Fungal Exudates
title Partial Resistance of Carrot to Alternaria dauci Correlates with In Vitro Cultured Carrot Cell Resistance to Fungal Exudates
title_full Partial Resistance of Carrot to Alternaria dauci Correlates with In Vitro Cultured Carrot Cell Resistance to Fungal Exudates
title_fullStr Partial Resistance of Carrot to Alternaria dauci Correlates with In Vitro Cultured Carrot Cell Resistance to Fungal Exudates
title_full_unstemmed Partial Resistance of Carrot to Alternaria dauci Correlates with In Vitro Cultured Carrot Cell Resistance to Fungal Exudates
title_short Partial Resistance of Carrot to Alternaria dauci Correlates with In Vitro Cultured Carrot Cell Resistance to Fungal Exudates
title_sort partial resistance of carrot to alternaria dauci correlates with in vitro cultured carrot cell resistance to fungal exudates
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4077726/
https://www.ncbi.nlm.nih.gov/pubmed/24983469
http://dx.doi.org/10.1371/journal.pone.0101008
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