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Proteomic analysis of effects by x-rays and heavy ion in HeLa cells
BACKGROUND: Carbon ion therapy may be better against cancer than the effects of a photon beam. To investigate a biological advantage of carbon ion beam over X-rays, the radioresistant cell line HeLa cells were used. Radiation-induced changes in the biological processes were investigated post-irradia...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Versita, Warsaw
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4078033/ https://www.ncbi.nlm.nih.gov/pubmed/24991204 http://dx.doi.org/10.2478/raon-2013-0087 |
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author | Bing, Zhitong Yang, Guanghui Zhang, Yanan Wang, Fengling Ye, Caiyong Sun, Jintu Zhou, Guangming Yang, Lei |
author_facet | Bing, Zhitong Yang, Guanghui Zhang, Yanan Wang, Fengling Ye, Caiyong Sun, Jintu Zhou, Guangming Yang, Lei |
author_sort | Bing, Zhitong |
collection | PubMed |
description | BACKGROUND: Carbon ion therapy may be better against cancer than the effects of a photon beam. To investigate a biological advantage of carbon ion beam over X-rays, the radioresistant cell line HeLa cells were used. Radiation-induced changes in the biological processes were investigated post-irradiation at 1 h by a clinically relevant radiation dose (2 Gy X-ray and 2 Gy carbon beam). The differential expression proteins were collected for analysing biological effects. MATERIALS AND METHODS. The radioresistant cell line Hela cells were used. In our study, the stable isotope labelling with amino acids (SILAC) method coupled with 2D-LC-LTQ Orbitrap mass spectrometry was applied to identity and quantify the differentially expressed proteins after irradiation. The Western blotting experiment was used to validate the data. RESULTS: A total of 123 and 155 significantly changed proteins were evaluated with treatment of 2 Gy carbon and X-rays after radiation 1 h, respectively. These deregulated proteins were found to be mainly involved in several kinds of metabolism processes through Gene Ontology (GO) enrichment analysis. The two groups perform different response to different types of irradiation. CONCLUSIONS: The radioresistance of the cancer cells treated with 2 Gy X-rays irradiation may be largely due to glycolysis enhancement, while the greater killing effect of 2 Gy carbon may be due to unchanged glycolysis and decreased amino acid metabolism. |
format | Online Article Text |
id | pubmed-4078033 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Versita, Warsaw |
record_format | MEDLINE/PubMed |
spelling | pubmed-40780332014-07-02 Proteomic analysis of effects by x-rays and heavy ion in HeLa cells Bing, Zhitong Yang, Guanghui Zhang, Yanan Wang, Fengling Ye, Caiyong Sun, Jintu Zhou, Guangming Yang, Lei Radiol Oncol Research Article BACKGROUND: Carbon ion therapy may be better against cancer than the effects of a photon beam. To investigate a biological advantage of carbon ion beam over X-rays, the radioresistant cell line HeLa cells were used. Radiation-induced changes in the biological processes were investigated post-irradiation at 1 h by a clinically relevant radiation dose (2 Gy X-ray and 2 Gy carbon beam). The differential expression proteins were collected for analysing biological effects. MATERIALS AND METHODS. The radioresistant cell line Hela cells were used. In our study, the stable isotope labelling with amino acids (SILAC) method coupled with 2D-LC-LTQ Orbitrap mass spectrometry was applied to identity and quantify the differentially expressed proteins after irradiation. The Western blotting experiment was used to validate the data. RESULTS: A total of 123 and 155 significantly changed proteins were evaluated with treatment of 2 Gy carbon and X-rays after radiation 1 h, respectively. These deregulated proteins were found to be mainly involved in several kinds of metabolism processes through Gene Ontology (GO) enrichment analysis. The two groups perform different response to different types of irradiation. CONCLUSIONS: The radioresistance of the cancer cells treated with 2 Gy X-rays irradiation may be largely due to glycolysis enhancement, while the greater killing effect of 2 Gy carbon may be due to unchanged glycolysis and decreased amino acid metabolism. Versita, Warsaw 2014-04-25 /pmc/articles/PMC4078033/ /pubmed/24991204 http://dx.doi.org/10.2478/raon-2013-0087 Text en Copyright © by Association of Radiology & Oncology http://creativecommons.org/licenses/by/3.0 This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/). |
spellingShingle | Research Article Bing, Zhitong Yang, Guanghui Zhang, Yanan Wang, Fengling Ye, Caiyong Sun, Jintu Zhou, Guangming Yang, Lei Proteomic analysis of effects by x-rays and heavy ion in HeLa cells |
title | Proteomic analysis of effects by x-rays and heavy ion in HeLa cells |
title_full | Proteomic analysis of effects by x-rays and heavy ion in HeLa cells |
title_fullStr | Proteomic analysis of effects by x-rays and heavy ion in HeLa cells |
title_full_unstemmed | Proteomic analysis of effects by x-rays and heavy ion in HeLa cells |
title_short | Proteomic analysis of effects by x-rays and heavy ion in HeLa cells |
title_sort | proteomic analysis of effects by x-rays and heavy ion in hela cells |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4078033/ https://www.ncbi.nlm.nih.gov/pubmed/24991204 http://dx.doi.org/10.2478/raon-2013-0087 |
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