Cargando…
The inhibitory effect of heat treatment against epithelial-mesenchymal transition (EMT) in human pancreatic adenocarcinoma cell lines
Epithelial-mesenchymal transition (EMT) plays a crucial role in cancer metastasis. In this study, we evaluated the effect of heat treatment on tumor growth factor-β1 (TGF-β1)-induced EMT in pancreatic cancer cells and tried to ascertain the mechanism related to any observed effects. Human pancreatic...
Autores principales: | , , , , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
the Society for Free Radical Research Japan
2014
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4078073/ https://www.ncbi.nlm.nih.gov/pubmed/25120280 http://dx.doi.org/10.3164/jcbn.14-8 |
_version_ | 1782323692174311424 |
---|---|
author | Kimura-Tsuchiya, Reiko Ishikawa, Takeshi Kokura, Satoshi Mizushima, Katsura Adachi, Satoko Okajima, Manabu Matsuyama, Tatsuzo Okayama, Tetsuya Sakamoto, Naoyuki Katada, Kazuhiro Kamada, Kazuhiro Uchiyama, Kazuhiko Handa, Osamu Takagi, Tomohisa Yagi, Nobuaki Naito, Yuji Itoh, Yoshito |
author_facet | Kimura-Tsuchiya, Reiko Ishikawa, Takeshi Kokura, Satoshi Mizushima, Katsura Adachi, Satoko Okajima, Manabu Matsuyama, Tatsuzo Okayama, Tetsuya Sakamoto, Naoyuki Katada, Kazuhiro Kamada, Kazuhiro Uchiyama, Kazuhiko Handa, Osamu Takagi, Tomohisa Yagi, Nobuaki Naito, Yuji Itoh, Yoshito |
author_sort | Kimura-Tsuchiya, Reiko |
collection | PubMed |
description | Epithelial-mesenchymal transition (EMT) plays a crucial role in cancer metastasis. In this study, we evaluated the effect of heat treatment on tumor growth factor-β1 (TGF-β1)-induced EMT in pancreatic cancer cells and tried to ascertain the mechanism related to any observed effects. Human pancreatic cancer cell lines (BxPC-3, PANC-1 and MIAPaCa-2) were stimulated by TGF-β1, and evaluated for morphological changes using immunofluorescence and EMT-related factors (i.e., E-cadherin, Vimentin, Snail or ZEB-1) using RT-PCR. To examine the effect of heat on EMT, the cancer cells were heat-treated at 43°C for 1 h then stimulated with TGF-β1. We then evaluated whether or not heat treatment changed the expression of EMT-related factors and cell migration and also whether Smad activation was inhibited in TGF-β signaling. After being treated with TGF-β1, pancreatic cancer cells resulted in EMT and cell migration was enhanced. Heat treatment inhibited TGF-β1-induced changes in morphology, inhibited the expression of EMT-related factors, and attenuated TGF-β1-induced migration in pancreatic cancer cells. Additionally, we observed that heat treatment blocked TGF-β1-induced phosphorylation of Smad2 in PANC-1 cells. Our results suggest that heat treatment can suppress TGF-β1-induced EMT and opens the possibility of a new therapeutic use of hyperthermia as a potential treatment for cancer metastasis. |
format | Online Article Text |
id | pubmed-4078073 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | the Society for Free Radical Research Japan |
record_format | MEDLINE/PubMed |
spelling | pubmed-40780732014-08-12 The inhibitory effect of heat treatment against epithelial-mesenchymal transition (EMT) in human pancreatic adenocarcinoma cell lines Kimura-Tsuchiya, Reiko Ishikawa, Takeshi Kokura, Satoshi Mizushima, Katsura Adachi, Satoko Okajima, Manabu Matsuyama, Tatsuzo Okayama, Tetsuya Sakamoto, Naoyuki Katada, Kazuhiro Kamada, Kazuhiro Uchiyama, Kazuhiko Handa, Osamu Takagi, Tomohisa Yagi, Nobuaki Naito, Yuji Itoh, Yoshito J Clin Biochem Nutr Original Article Epithelial-mesenchymal transition (EMT) plays a crucial role in cancer metastasis. In this study, we evaluated the effect of heat treatment on tumor growth factor-β1 (TGF-β1)-induced EMT in pancreatic cancer cells and tried to ascertain the mechanism related to any observed effects. Human pancreatic cancer cell lines (BxPC-3, PANC-1 and MIAPaCa-2) were stimulated by TGF-β1, and evaluated for morphological changes using immunofluorescence and EMT-related factors (i.e., E-cadherin, Vimentin, Snail or ZEB-1) using RT-PCR. To examine the effect of heat on EMT, the cancer cells were heat-treated at 43°C for 1 h then stimulated with TGF-β1. We then evaluated whether or not heat treatment changed the expression of EMT-related factors and cell migration and also whether Smad activation was inhibited in TGF-β signaling. After being treated with TGF-β1, pancreatic cancer cells resulted in EMT and cell migration was enhanced. Heat treatment inhibited TGF-β1-induced changes in morphology, inhibited the expression of EMT-related factors, and attenuated TGF-β1-induced migration in pancreatic cancer cells. Additionally, we observed that heat treatment blocked TGF-β1-induced phosphorylation of Smad2 in PANC-1 cells. Our results suggest that heat treatment can suppress TGF-β1-induced EMT and opens the possibility of a new therapeutic use of hyperthermia as a potential treatment for cancer metastasis. the Society for Free Radical Research Japan 2014-07 2014-05-13 /pmc/articles/PMC4078073/ /pubmed/25120280 http://dx.doi.org/10.3164/jcbn.14-8 Text en Copyright © 2014 JCBN This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Kimura-Tsuchiya, Reiko Ishikawa, Takeshi Kokura, Satoshi Mizushima, Katsura Adachi, Satoko Okajima, Manabu Matsuyama, Tatsuzo Okayama, Tetsuya Sakamoto, Naoyuki Katada, Kazuhiro Kamada, Kazuhiro Uchiyama, Kazuhiko Handa, Osamu Takagi, Tomohisa Yagi, Nobuaki Naito, Yuji Itoh, Yoshito The inhibitory effect of heat treatment against epithelial-mesenchymal transition (EMT) in human pancreatic adenocarcinoma cell lines |
title | The inhibitory effect of heat treatment against epithelial-mesenchymal transition (EMT) in human pancreatic adenocarcinoma cell lines |
title_full | The inhibitory effect of heat treatment against epithelial-mesenchymal transition (EMT) in human pancreatic adenocarcinoma cell lines |
title_fullStr | The inhibitory effect of heat treatment against epithelial-mesenchymal transition (EMT) in human pancreatic adenocarcinoma cell lines |
title_full_unstemmed | The inhibitory effect of heat treatment against epithelial-mesenchymal transition (EMT) in human pancreatic adenocarcinoma cell lines |
title_short | The inhibitory effect of heat treatment against epithelial-mesenchymal transition (EMT) in human pancreatic adenocarcinoma cell lines |
title_sort | inhibitory effect of heat treatment against epithelial-mesenchymal transition (emt) in human pancreatic adenocarcinoma cell lines |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4078073/ https://www.ncbi.nlm.nih.gov/pubmed/25120280 http://dx.doi.org/10.3164/jcbn.14-8 |
work_keys_str_mv | AT kimuratsuchiyareiko theinhibitoryeffectofheattreatmentagainstepithelialmesenchymaltransitionemtinhumanpancreaticadenocarcinomacelllines AT ishikawatakeshi theinhibitoryeffectofheattreatmentagainstepithelialmesenchymaltransitionemtinhumanpancreaticadenocarcinomacelllines AT kokurasatoshi theinhibitoryeffectofheattreatmentagainstepithelialmesenchymaltransitionemtinhumanpancreaticadenocarcinomacelllines AT mizushimakatsura theinhibitoryeffectofheattreatmentagainstepithelialmesenchymaltransitionemtinhumanpancreaticadenocarcinomacelllines AT adachisatoko theinhibitoryeffectofheattreatmentagainstepithelialmesenchymaltransitionemtinhumanpancreaticadenocarcinomacelllines AT okajimamanabu theinhibitoryeffectofheattreatmentagainstepithelialmesenchymaltransitionemtinhumanpancreaticadenocarcinomacelllines AT matsuyamatatsuzo theinhibitoryeffectofheattreatmentagainstepithelialmesenchymaltransitionemtinhumanpancreaticadenocarcinomacelllines AT okayamatetsuya theinhibitoryeffectofheattreatmentagainstepithelialmesenchymaltransitionemtinhumanpancreaticadenocarcinomacelllines AT sakamotonaoyuki theinhibitoryeffectofheattreatmentagainstepithelialmesenchymaltransitionemtinhumanpancreaticadenocarcinomacelllines AT katadakazuhiro theinhibitoryeffectofheattreatmentagainstepithelialmesenchymaltransitionemtinhumanpancreaticadenocarcinomacelllines AT kamadakazuhiro theinhibitoryeffectofheattreatmentagainstepithelialmesenchymaltransitionemtinhumanpancreaticadenocarcinomacelllines AT uchiyamakazuhiko theinhibitoryeffectofheattreatmentagainstepithelialmesenchymaltransitionemtinhumanpancreaticadenocarcinomacelllines AT handaosamu theinhibitoryeffectofheattreatmentagainstepithelialmesenchymaltransitionemtinhumanpancreaticadenocarcinomacelllines AT takagitomohisa theinhibitoryeffectofheattreatmentagainstepithelialmesenchymaltransitionemtinhumanpancreaticadenocarcinomacelllines AT yaginobuaki theinhibitoryeffectofheattreatmentagainstepithelialmesenchymaltransitionemtinhumanpancreaticadenocarcinomacelllines AT naitoyuji theinhibitoryeffectofheattreatmentagainstepithelialmesenchymaltransitionemtinhumanpancreaticadenocarcinomacelllines AT itohyoshito theinhibitoryeffectofheattreatmentagainstepithelialmesenchymaltransitionemtinhumanpancreaticadenocarcinomacelllines AT kimuratsuchiyareiko inhibitoryeffectofheattreatmentagainstepithelialmesenchymaltransitionemtinhumanpancreaticadenocarcinomacelllines AT ishikawatakeshi inhibitoryeffectofheattreatmentagainstepithelialmesenchymaltransitionemtinhumanpancreaticadenocarcinomacelllines AT kokurasatoshi inhibitoryeffectofheattreatmentagainstepithelialmesenchymaltransitionemtinhumanpancreaticadenocarcinomacelllines AT mizushimakatsura inhibitoryeffectofheattreatmentagainstepithelialmesenchymaltransitionemtinhumanpancreaticadenocarcinomacelllines AT adachisatoko inhibitoryeffectofheattreatmentagainstepithelialmesenchymaltransitionemtinhumanpancreaticadenocarcinomacelllines AT okajimamanabu inhibitoryeffectofheattreatmentagainstepithelialmesenchymaltransitionemtinhumanpancreaticadenocarcinomacelllines AT matsuyamatatsuzo inhibitoryeffectofheattreatmentagainstepithelialmesenchymaltransitionemtinhumanpancreaticadenocarcinomacelllines AT okayamatetsuya inhibitoryeffectofheattreatmentagainstepithelialmesenchymaltransitionemtinhumanpancreaticadenocarcinomacelllines AT sakamotonaoyuki inhibitoryeffectofheattreatmentagainstepithelialmesenchymaltransitionemtinhumanpancreaticadenocarcinomacelllines AT katadakazuhiro inhibitoryeffectofheattreatmentagainstepithelialmesenchymaltransitionemtinhumanpancreaticadenocarcinomacelllines AT kamadakazuhiro inhibitoryeffectofheattreatmentagainstepithelialmesenchymaltransitionemtinhumanpancreaticadenocarcinomacelllines AT uchiyamakazuhiko inhibitoryeffectofheattreatmentagainstepithelialmesenchymaltransitionemtinhumanpancreaticadenocarcinomacelllines AT handaosamu inhibitoryeffectofheattreatmentagainstepithelialmesenchymaltransitionemtinhumanpancreaticadenocarcinomacelllines AT takagitomohisa inhibitoryeffectofheattreatmentagainstepithelialmesenchymaltransitionemtinhumanpancreaticadenocarcinomacelllines AT yaginobuaki inhibitoryeffectofheattreatmentagainstepithelialmesenchymaltransitionemtinhumanpancreaticadenocarcinomacelllines AT naitoyuji inhibitoryeffectofheattreatmentagainstepithelialmesenchymaltransitionemtinhumanpancreaticadenocarcinomacelllines AT itohyoshito inhibitoryeffectofheattreatmentagainstepithelialmesenchymaltransitionemtinhumanpancreaticadenocarcinomacelllines |