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The inhibitory effect of heat treatment against epithelial-mesenchymal transition (EMT) in human pancreatic adenocarcinoma cell lines

Epithelial-mesenchymal transition (EMT) plays a crucial role in cancer metastasis. In this study, we evaluated the effect of heat treatment on tumor growth factor-β1 (TGF-β1)-induced EMT in pancreatic cancer cells and tried to ascertain the mechanism related to any observed effects. Human pancreatic...

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Autores principales: Kimura-Tsuchiya, Reiko, Ishikawa, Takeshi, Kokura, Satoshi, Mizushima, Katsura, Adachi, Satoko, Okajima, Manabu, Matsuyama, Tatsuzo, Okayama, Tetsuya, Sakamoto, Naoyuki, Katada, Kazuhiro, Kamada, Kazuhiro, Uchiyama, Kazuhiko, Handa, Osamu, Takagi, Tomohisa, Yagi, Nobuaki, Naito, Yuji, Itoh, Yoshito
Formato: Online Artículo Texto
Lenguaje:English
Publicado: the Society for Free Radical Research Japan 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4078073/
https://www.ncbi.nlm.nih.gov/pubmed/25120280
http://dx.doi.org/10.3164/jcbn.14-8
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author Kimura-Tsuchiya, Reiko
Ishikawa, Takeshi
Kokura, Satoshi
Mizushima, Katsura
Adachi, Satoko
Okajima, Manabu
Matsuyama, Tatsuzo
Okayama, Tetsuya
Sakamoto, Naoyuki
Katada, Kazuhiro
Kamada, Kazuhiro
Uchiyama, Kazuhiko
Handa, Osamu
Takagi, Tomohisa
Yagi, Nobuaki
Naito, Yuji
Itoh, Yoshito
author_facet Kimura-Tsuchiya, Reiko
Ishikawa, Takeshi
Kokura, Satoshi
Mizushima, Katsura
Adachi, Satoko
Okajima, Manabu
Matsuyama, Tatsuzo
Okayama, Tetsuya
Sakamoto, Naoyuki
Katada, Kazuhiro
Kamada, Kazuhiro
Uchiyama, Kazuhiko
Handa, Osamu
Takagi, Tomohisa
Yagi, Nobuaki
Naito, Yuji
Itoh, Yoshito
author_sort Kimura-Tsuchiya, Reiko
collection PubMed
description Epithelial-mesenchymal transition (EMT) plays a crucial role in cancer metastasis. In this study, we evaluated the effect of heat treatment on tumor growth factor-β1 (TGF-β1)-induced EMT in pancreatic cancer cells and tried to ascertain the mechanism related to any observed effects. Human pancreatic cancer cell lines (BxPC-3, PANC-1 and MIAPaCa-2) were stimulated by TGF-β1, and evaluated for morphological changes using immunofluorescence and EMT-related factors (i.e., E-cadherin, Vimentin, Snail or ZEB-1) using RT-PCR. To examine the effect of heat on EMT, the cancer cells were heat-treated at 43°C for 1 h then stimulated with TGF-β1. We then evaluated whether or not heat treatment changed the expression of EMT-related factors and cell migration and also whether Smad activation was inhibited in TGF-β signaling. After being treated with TGF-β1, pancreatic cancer cells resulted in EMT and cell migration was enhanced. Heat treatment inhibited TGF-β1-induced changes in morphology, inhibited the expression of EMT-related factors, and attenuated TGF-β1-induced migration in pancreatic cancer cells. Additionally, we observed that heat treatment blocked TGF-β1-induced phosphorylation of Smad2 in PANC-1 cells. Our results suggest that heat treatment can suppress TGF-β1-induced EMT and opens the possibility of a new therapeutic use of hyperthermia as a potential treatment for cancer metastasis.
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spelling pubmed-40780732014-08-12 The inhibitory effect of heat treatment against epithelial-mesenchymal transition (EMT) in human pancreatic adenocarcinoma cell lines Kimura-Tsuchiya, Reiko Ishikawa, Takeshi Kokura, Satoshi Mizushima, Katsura Adachi, Satoko Okajima, Manabu Matsuyama, Tatsuzo Okayama, Tetsuya Sakamoto, Naoyuki Katada, Kazuhiro Kamada, Kazuhiro Uchiyama, Kazuhiko Handa, Osamu Takagi, Tomohisa Yagi, Nobuaki Naito, Yuji Itoh, Yoshito J Clin Biochem Nutr Original Article Epithelial-mesenchymal transition (EMT) plays a crucial role in cancer metastasis. In this study, we evaluated the effect of heat treatment on tumor growth factor-β1 (TGF-β1)-induced EMT in pancreatic cancer cells and tried to ascertain the mechanism related to any observed effects. Human pancreatic cancer cell lines (BxPC-3, PANC-1 and MIAPaCa-2) were stimulated by TGF-β1, and evaluated for morphological changes using immunofluorescence and EMT-related factors (i.e., E-cadherin, Vimentin, Snail or ZEB-1) using RT-PCR. To examine the effect of heat on EMT, the cancer cells were heat-treated at 43°C for 1 h then stimulated with TGF-β1. We then evaluated whether or not heat treatment changed the expression of EMT-related factors and cell migration and also whether Smad activation was inhibited in TGF-β signaling. After being treated with TGF-β1, pancreatic cancer cells resulted in EMT and cell migration was enhanced. Heat treatment inhibited TGF-β1-induced changes in morphology, inhibited the expression of EMT-related factors, and attenuated TGF-β1-induced migration in pancreatic cancer cells. Additionally, we observed that heat treatment blocked TGF-β1-induced phosphorylation of Smad2 in PANC-1 cells. Our results suggest that heat treatment can suppress TGF-β1-induced EMT and opens the possibility of a new therapeutic use of hyperthermia as a potential treatment for cancer metastasis. the Society for Free Radical Research Japan 2014-07 2014-05-13 /pmc/articles/PMC4078073/ /pubmed/25120280 http://dx.doi.org/10.3164/jcbn.14-8 Text en Copyright © 2014 JCBN This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Kimura-Tsuchiya, Reiko
Ishikawa, Takeshi
Kokura, Satoshi
Mizushima, Katsura
Adachi, Satoko
Okajima, Manabu
Matsuyama, Tatsuzo
Okayama, Tetsuya
Sakamoto, Naoyuki
Katada, Kazuhiro
Kamada, Kazuhiro
Uchiyama, Kazuhiko
Handa, Osamu
Takagi, Tomohisa
Yagi, Nobuaki
Naito, Yuji
Itoh, Yoshito
The inhibitory effect of heat treatment against epithelial-mesenchymal transition (EMT) in human pancreatic adenocarcinoma cell lines
title The inhibitory effect of heat treatment against epithelial-mesenchymal transition (EMT) in human pancreatic adenocarcinoma cell lines
title_full The inhibitory effect of heat treatment against epithelial-mesenchymal transition (EMT) in human pancreatic adenocarcinoma cell lines
title_fullStr The inhibitory effect of heat treatment against epithelial-mesenchymal transition (EMT) in human pancreatic adenocarcinoma cell lines
title_full_unstemmed The inhibitory effect of heat treatment against epithelial-mesenchymal transition (EMT) in human pancreatic adenocarcinoma cell lines
title_short The inhibitory effect of heat treatment against epithelial-mesenchymal transition (EMT) in human pancreatic adenocarcinoma cell lines
title_sort inhibitory effect of heat treatment against epithelial-mesenchymal transition (emt) in human pancreatic adenocarcinoma cell lines
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4078073/
https://www.ncbi.nlm.nih.gov/pubmed/25120280
http://dx.doi.org/10.3164/jcbn.14-8
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